Quality Control in Medicinal Mushroom Industry?

Medicinal mushroom extracts are all the rage nowadays due to their numerous functional benefits. Mushroom extracts not only taste delicious, but offer benefits such as enhanced immune function, better cognitive performance, and improved exercise performance. However, when it comes to medicinal mushroom extracts, not all products are made the same. How does one know if a product will actually benefit them? The answer to this question lies in quality control. In this blog, we will explore the need for quality control in the mushroom extract industry, the hidden risks of low-quality products, and what to look for to make sure you’re getting the real deal.

What are Medicinal Mushroom Extracts?

A common question which comes up when interacting with patrons is: “Why do I need extracts? Can’t I consume the mushroom directly?” This is a legitimate question which deserves a legitimate answer. When it comes to mushrooms, most of the bioactive compounds which provide functional benefits are sheltered within the cells of the mushroom. These molecules are protected by a tough cell wall made of polysaccharides and chitin (Check out our blog on mushroom cell walls). 

The cell wall is designed to protect the inner contents of the fungal cell and make it difficult to access by a predator or pathogen. This tough casing is also how mushrooms survive in a wide range of climatic conditions. The solidity of this cell wall varies for different species of mushrooms. Some mushrooms like Reishi (Ganoderma lucidum) have an extremely tough and woody fruiting body, making them difficult to digest, while others like Jelly fungi (Tremella sp.) have a smooth, gel-like texture.

Fig 1: Mushroom powders are made of  whole dried mushrooms ground into a fine powder (image)

Why Choose Mushroom Extracts Over Whole Mushrooms?

Mushroom powders or whole mushrooms contain all components of the mushroom, including the cell wall. Human digestive systems are not good at breaking down components such as chitin. This makes the bioactive molecules of a mushroom difficult to access when consumed directly. Mushroom extracts, on the other hand, undergo a series of processing steps which draw out the bioactive molecules such as beta glucans, terpenes and other molecules, thus making them more accessible in our digestive system. (Check out Nuvedo’s mushroom tinctures)

Fig 2: Mushroom extracts are an easy way to consume isolated bioactive compounds without having to digest tough mushroom cell walls (image)

The Extraction Process: Unlocking Bioactive Compounds

Traditionally mushrooms are extracted using a combination of water and alcohol as the solvents. This is done to ensure that the greatest number of molecules are extracted as some molecules are more soluble in water and others are more soluble in alcohol. Since most bioactive molecules are locked within the mushroom cell wall, various methods are used to draw out bioactive compounds from the mushrooms. (Our blog on mushroom cell walls highlights the importance of extraction).

Fig 3: Mushroom being extracted by boiling in a vat of hot water (image)

Hot water extracts

The most common form of extraction utilises heat and water to extract water soluble compounds from the mushroom. This is often known as hot water extraction and involves boiling the mushrooms in water for long periods of time, almost like a mushroom tea. Water soluble compounds majorly include- 

1. Polysaccharides: Beta-glucans (immune-boosting), mannans, and partially soluble chitin.
2. Proteins: Lectins (immune-regulating) and enzymes like laccases.
3. Amino Acids: Essential amino acids and glutamic acid (umami flavor).
4. Vitamins: B-vitamins (B2, B3, B5, biotin) and small amounts of vitamin C.
5. Phenolic Compounds: Antioxidants like gallic acid and catechins.
6. Minerals: Potassium, magnesium, phosphorus, and trace elements like selenium.
7. Nucleotides: Adenosine and guanosine (umami and neurological benefits).
8. Sugars and Organic Acids: Trehalose, mannitol, malic acid, and citric acid.

These compounds are commonly extracted using hot water for functional products like teas and tinctures.

Alcohol extracts or Tinctures

Alcoholic extraction is often done using a very high proof of alcohol to ensure maximum extraction. The mushrooms are traditionally soaked in alcohol and left untouched for weeks to allow the active molecules to be slowly drawn out from within the cells. This is a long process but results in potent mushroom extracts. Some simmer the mushrooms in ethanol the same way they manufacture hot water extracts, but this process isn’t ideal as many of the mushroom’s bioactives, such as terpenes, are volatile in nature and sensitive to high temperatures. Here are some of the prominent alcohol soluble compounds in mushrooms: 

1. Terpenoids (Triterpenes & Others): Includes compounds like ganoderic acids (Reishi), hericenones (Lion’s Mane), and inotodiol (Chaga) with anti-inflammatory, adaptogenic, and neuroprotective properties.
2. Sterols: Ergosterol (vitamin D precursor) and other sterol derivatives supporting cholesterol regulation and hormonal health.
3. Phenolic Compounds: Antioxidants like hispidin (Chaga) and other bioactive polyphenols.
4. Fatty Acids: Essential lipids that promote brain health and cellular function.
5. Alkaloids: Bioactive compounds with neuroprotective and therapeutic potential (present in select species).
6. Resins and Oils: Found in mushrooms like Chaga, contributing to anti-inflammatory and antioxidant effects.

Fig 4: Tinctures are made by soaking dried mushrooms in alcohol for long periods of time to draw out bioactive molecules (image)

Dual extracts – Best of both worlds

In an ideal scenario, the alcohol and water extracts are concentrated by boiling off the solvent (water or alcohol) to result in a more potent extract. These are then mixed together to result in a Dual Extract. The dual extraction method allows for the presence of active molecules extracted by both water and alcohol,  leading to a more wholesome physiological response due to a wider range of bioactive molecules in the final product. Some brands skip the concentration phase and just combine the water and ethanol extracts as is; this leads to very diluted extracts which have low potency ratios such as 1:20 ( where there is 1g of mushroom in every 20ml) compared to a more potent ratio, 1:5 in the case of Nuvedo extracts.

Fig 5: Ultrasound assisted extraction uses high energy sound waves to isolate bioactive compounds resulting in short extraction times (image)

Ultrasound assisted extraction 

At Nuvedo, we believe in the value of bioavailability. In our quest to improve extraction processes and make our products more easier to absorb, we have developed our very own ultrasound assisted extraction method to gently and efficiently draw out the bioactive molecules from within the cells of the mushroom. This method makes use of high frequency sound waves to bombard the cells of the mushroom and break open the cell wall without the use of high temperatures as is done during traditional hot water or hot ethanol extractions. Once the cell walls are broken, the bioactive compounds can be easily dissolved in the solvents such as water and ethanol, leading to a much gentler process of extraction with much lower extraction times. Combining the dual extraction method with ultrasound assisted extraction has helped us generate potent extracts with high levels of bioactive molecules.

A note on potency

A question that often arises when we talk about extraction is that of potency. Potency is defined as “the power of something to influence or affect”. The more potent a mushroom extract is, the more functional benefits it is thought to provide. The potency of an extract is decided not only by the amount of bioactive molecules in the product but also on how easily they are absorbed within the body. As such, mushroom extracts tend to be more potent than mushroom powders or whole mushrooms. And among extracts, Ultrasound assisted extracts tend to be more bioavailable for consumption compared to traditional extracts. (https://dergipark.org.tr/en/download/article-file/756779)

Ensuring Quality Control in Medicinal Mushroom Extracts

Quality is at the core of our company ethos at Nuvedo. We strive to ensure that every product and process we follow meets strict quality measures. Quality in the context of mushroom extracts means: quality of all inputs used, robustness of the process to ensure safety and consistency, and finally, ensuring that the end result meets global standards for safety and potency. Our emphasis on quality starts from the beginning of our process, in the humble belly of our cultivation lab.

Fig 6: Strains are unique varieties of any mushroom species which have desirable properties. Strains are generally maintained in a lab by growing them on a nutrient rich gel (image) 

Strain Selection

A strain is a unique genetic variant of a mushroom species which maintains its characteristics. They are similar to different breeds of dogs or varieties of mangoes. Strains are the bedrock of a good mushroom extract. Different strains have different properties such as the amounts of bioactive compounds present in them. The selection of the right strains is a crucial aspect of mushroom extract development. At Nuvedo, we meticulously test and identify the best strains for mushroom extract production so we can produce extracts with high concentrations of bioactive molecules. Regular genetic sequencing supports our workflow by ensuring the strains do not change over time.

Fig 7: Spawn is the starting material for mushroom cultivation and uses highly nutritious grains to maintain healthy mecelium (image)

Spawn Production

In mushroom cultivation, spawn is similar to the seeds of plants. Spawn production forms a core vertical at Nuvedo. The quality of the spawn goes a long way in ensuring high yields and minimising contamination. At Nuvedo, we prioritise spawn quality and make all of our spawn in-house. We choose the highest quality grains available in the market and follow a standard operating protocol to ensure that each batch of spawn is healthy and in the optimal stage of growth for propagation. Each batch of spawn is barcoded in a way in which it can be traced back to the mother culture used to make it. This gives us exceptional control over the cultivation process and helps us ensure the mushrooms we grow are of the highest quality. (To learn more about spawn production check out this blog)

Fig 8: A variety of different substrates can be used to grow mushrooms. Most commonly different sources of agricultural waste are utilised as substrates for mushroom cultivation (image)

Substrate Preparation

Substrates are the nutrients mushroom producing fungi feed on in their growing stages. The mycelia of fungi grow over substrates and extract nutrition from them by secreting proteins which digest the substrate. These nutrients are then taken up by the mycelium and support their growth. Different strains or species of mushrooms have their own unique nutrient requirements. Substrate composition is a crucial aspect of mushroom cultivation and plays a big role in enabling the production of bioactive molecules within the mushroom. Our proprietary substrate blends are designed to support maximum yields and bioactive compounds in all the different mushrooms we work with. We source locally available agricultural waste for use as substrate with a different recipe for each mushroom we work with.

Fig 9: Mushroom cultivation is performed is best performed under controlled conditions where factors such as temperature, humidity and light exposure are modulated (image)

Cultivation

A happy mushroom is a potent mushroom. Every strain has its own preferred climatic conditions within which it thrives. Crucial parameters like temperature, humidity and oxygen supply need to be maintained for reproducible yields and quality. These conditions are closely monitored along with daily inspections of the fruiting bags to prevent contaminants from creeping in. The stage at which mushrooms are harvested also play a critical role in determining the bioactive contents. We strive to ensure our mushrooms are harvested at the right stage of their growth for our extraction processes. All our cultivation protocols are customized and standardized to deliver the best results from each of the strains we work with.

Fig 10: Mushrooms are dried to extend their shelf life and reduce their volume for storage (image)

Drying

Fresh mushrooms have a low shelf life and can often begin to rot after a few days. Moreover, many of the bioactive compounds begin to break down with long storage periods. To prevent this, our mushrooms are dried at low temperatures in-house using our heat-pump dryers. Drying at low temperatures helps prevent the breakdown of bioactive compounds during the drying process. 

Extraction

Medicinal mushroom extracts are known for their potency and effectiveness due to bioactive compounds like beta-glucans. Over the years, we have engaged in constant research and development to produce the most potent and bioavailable mushroom extracts. Using a proprietary ultrasound based extraction process, dual extractions with alcohol and water draw out the maximum bioactive compounds from our dried mushrooms. Gentle concentration processes such as vacuum evaporation are used to increase the potency of the extracts. This process makes use of a vacuum to speed up the evaporation rate at low temperatures, preserving sensitive bioactive molecules such as terpenes. The extraction process parameters are standardized for each mushroom in order to get the best result in each case. These parameters include frequency of extraction, time of extraction, temperature of extraction, and ratio of solvents used. The water used in the extraction process is R.O. filtered and purified and the ethanol used is Extra-Neutral-Alcohol with a verified Certificiate Of Analysis. All the containers that come in contact with the extracts are made from borosilicate glass. Furthermore, all units of machinery used in extraction are built using 316 grade of stainless steel, which is resistant to corrosion. 

Packaging

Packaging is the final step in the production process. Sanitary packaging practices are the final but crucial step to prevent contamination and maintain extract quality. All our extracts are bottled inside a clean room under a HEPA filter, which prevents any external contamination from entering the packaging. This is a necessary step as we do not use any preservatives in our products. The bottles are then tightly sealed and shrink wrapped for a safe journey to your doorstep. Our liquid extracts are packed in special amber bottles to minimise exposure to UV radiation, which can degrade the extracts. Our extract powders are packed in food safe aluminium lined plastic bags with silica gel and oxygen absorbers to keep the powders safe from moisture and oxygen exposure.

Quality Control in Mushroom Extracts: Key Practices

The only way to determine the safety and effectiveness of a mushroom extract is through testing. These tests measure different quality parameters such as amounts of bioactive compounds, contamination, and presence of harmful chemicals like heavy metals. Adhering to rigorous quality control practices is crucial in order to meet consumer expectations and regulatory standards. While there may be a number of ways to test a given extract, the selection of the correct tests is important. In the following sections, we will take a look at the best testing practices and what to look out for when buying high quality extracts. Tests can be broadly classified under 3 categories: Potency, Safety and Authenticity 

Potency: How are mushroom extracts tested for bioactive molecules?

The main bioactive compounds in mushroom extracts are terpenes, polysaccharides and phenolics. The kind and composition of bioactive molecules varies from species to species and also strain to strain. Testing for levels of bioactive compounds is crucial for ensuring the effectiveness of mushroom extracts. High quality extracts provide more bang for your buck as they have higher potency and purity.

Fig 11: The phenol sulphuric acid method is used as a method to determine polysaccharide levels in mushroom extracts. Polysaccharides are reacted with sulphuric acid and phenol to generate a coloured compound which is measured using light of a specific wavelength (image)

Testing for Polysaccharides

Polysaccharides are long chains of carbohydrate molecules which form essential components of mushroom cell walls. They are responsible for the immunomodulatory effects of mushroom extracts. Polysaccharides also act as indigestible fiber. A simple test known as phenol sulphuric acid assay can be used for determining polysaccharide levels.

Fig 12: Megazyme beta glucan assay kit is used to quantify beta glucan content in mushroom samples (image)

Testing for Beta glucans

Beta glucans are a type of polysaccharide molecules which are abundant in mushrooms and other fungi. They have potent immunomodulatory properties. Beta glucans can be found in all mushroom extracts. Beta glucans from Reishi mushrooms in particular have been well studied for their effects on the immune system. They help reduce inflammation while enhancing the antimicrobial activity of the immune system. (check out our blog on beta glucans to know more!)

Beta glucans are commonly tested using a beta glucan testing kit developed by Megazyme. The kit uses a variety of enzymes and colour forming reagents to determine beta glucan levels from mushroom samples. 

 

Fig 13: An example of an HPLC chromatogram used to identify terpene composition of botanical extracts (image)

Testing for Terpenes

Terpenes are responsible for unique properties of mushroom extracts such as the neuroprotective and brain-boosting effects of Lion’s mane extracts or the stress relief effects of Reishi mushrooms. Many different kinds of terpenes are present in mushroom extracts and testing for each one of them individually is a difficult and expensive process. A more general terpene detection test such as a vanillin-glacial acetic acid assay can provide quick and reliable results at lower prices.

Fig 14: Folin-Ciocalteau assay is used as a quantitative test for phenolic content (image)

Testing for Phenolics

Phenolic molecules are best known for their antioxidant activity. They are extremely diverse and susceptible to degradation. Due to their antioxidant activities they help the body fight against oxidative damage. This helps cells maintain healthy DNA and protein leading to better cellular function and reduced damage as we age.

Safety: How to know if an extract is safe to consume?

Safety testing is especially important when it comes to mushroom extracts. Mushrooms are known to sequester or absorb nutrients as well as toxins from the environment and substrates on which they are grown. This can often lead to mushroom extracts have high levels of toxic compounds if they are not produced in sanitary conditions.

Fig 15: Method of testing microbial load in a given sample. Test samples are spread on nutrient medium and presence of microbial growth is observed. (image)

Testing for microbial load

Microbes such as pathogenic bacteria can be extremely harmful when consumed orally. Microbial load testing is performed to measure the levels of harmful bacteria in mushroom extracts. Traditionally testing is performed for two common species of pathogenic bacteria: Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These pathogens should be absent from mushroom extracts to consider them safe for consumption

Fig 16: Heavy metal testing is performed using a method known as ICP-MS where charged metal ions are separated based on their size and detected (image)

Heavy metal testing

Heavy metals such as Lead, Arsenic and Mercury are known to have harmful effects when consumed. They are often found in contaminated water and when this water is used for mushroom cultivation or extract production, these heavy metals contaminate the final product. Using RO (reverse osmosis) purified water is the best way to limit heavy metal contamination in mushroom extracts. 

Mushrooms are exceptionally good at absorbing contaminants from their substrates and concentrating them in mycelia and fruiting bodies. Knowing the heavy metal content of mushrooms and their extracts is crucial for safety. Consuming higher than permissible limits of heavy metals can lead to serious health issues.

Fig 17: DNA testing is performed using a method known as Sanger sequencing where a region of the DNA is amplified and the letters are read using a sequencing instrument (image)

Authenticity: DNA Testing

DNA testing is performed to ensure the right strains are being used for the mushroom cultivation and extraction processes. Periodic DNA testing ensures that the strains used are not getting contaminated and allows for  a similar result across multiple batches of production. Most commonly, the ITS region of the fungal genome is used for testing using a method known as Sanger sequencing. 

Certifications

Companies pursue certifications like HACCP, ISO 9001:2015, and FDA compliance to uphold the highest standards of safety, quality, and consumer trust. These certifications involve systematic processes, including identifying risks, establishing control measures, and monitoring production at every stage. At Nuvedo, we’ve implemented HACCP (Hazard Analysis and Critical Control Points) to proactively assess and manage potential hazards in our operations, ensuring that our products meet stringent safety criteria. Our ISO 9001:2015 certification emphasizes our commitment to a robust quality management system, focusing on consistent improvement, efficiency, and customer satisfaction. Furthermore, achieving FDA compliance signifies adherence to international safety, labeling, and regulatory standards, allowing us to meet global market requirements. For our customers, these certifications are a testament to our unwavering dedication to producing safe, reliable, and top-quality functional mushroom products that they can trust.

Conclusion: The Importance of Quality in Mushroom Supplements

Quality control is undoubtedly an essential part of the mushroom extract production process. In a nascent industry, government regulation is more focused on safety. Heavy metal and microbial testing is mandated by government bodies such as FSSAI in our case to ensure safety of mushroom derived products. However, testing for bioactive compounds is not necessary to satisfy government regulations. This lack of emphasis on bioactive molecule testing leads to ineffective products flooding the market and creates doubt over the impact of mushroom extracts on health and wellbeing in the minds of consumers.

Over the last few years, we at Nuvedo have been working with a number of accredited testing agencies to develop a comprehensive panel specially designed for testing of bioactive compounds in mushroom extracts. The panel especially measures the amounts of polysaccharides, terpenes and phenolics in mushroom extracts. With the development of this panel we aim to lead the way for instilling trust in mushroom extract based products in consumers and promote the adoption of these quality control measures by other companies. 

Explore Nuvedo’s range of premium medicinal mushroom extracts and experience the difference today! 

 

FAQs (Frequently Asked Questions)

Q: Why are medicinal mushroom extracts better than powders?

A: Medicinal mushroom extracts are more potent because they undergo processes like dual extraction, which unlocks bioactive compounds such as beta-glucans and terpenes, making them easily absorbable.

Q: What is the difference between mushroom powders and extracts?

A: Mushroom powders are made by grinding or milling mushroom mycelium or fruiting bodies. They are similar to dried mushrooms in their composition. Mushroom extracts are prepared by drawing out bioactive compounds using solvents resulting in potent medicinal benefits when consumed in smaller quantities.

Q: How is the quality of mushroom extracts tested?

A: Mushroom extracts are tested to ensure quality by measuring the levels of bioactive compounds such as beta glucans, polysaccharides and terpenes. Tests for safety such as microbial load and presence of heavy metals are also performed.

Q: Are mushroom extracts safe?

A: Mushroom extracts are completely safe when prepared using sanitary protocols and standardised methods. Always look for safety testing results when buying mushroom extracts.

Q: How are mushroom extracts consumed?

A: Mushroom extracts can be consumed in a variety of ways. Liquid extracts are extremely versatile and can be consumed directly or by diluting them in any beverage of choice (tea, coffee, kombucha). They can also be added to soups and broths for an umami kick. Mushroom extract powders are commonly available as capsules or can be dissolved similarly to liquid extracts.

Q: Which is better: Mushroom extracts or powders?

A: Mushroom extracts are better than powders as the bioactive compounds are more easily absorbed  by the digestive system compared to powders. 

Q: Do mushroom extracts go bad?

A: Mushroom extracts can go bad if they are not safely formulated or stored. Powdered extracts can absorb moisture from the environment and spoil if they are not stored properly. Liquid extracts are generally formulated with glycerine or alcohol to prevent microbial growth.

Q: Is it good to take mushroom supplements everyday?

A: Yes, the beneficial effects of mushroom effects can be best observed when consuming them over long periods of time. Medicinal mushroom extracts support physiological processes in the body and bring cells to a state of optimum performance when consumed regularly.

Q: What are side effects of mushroom supplements?

A: Mushroom extracts are generally safe for most people and can be consumed on a daily basis. Side effects may arise due to allergic reactions or from contaminants present in improperly produced extracts. Always look for quality control reports when buying mushroom extracts.

Q: What are the benefits of consuming mushroom extracts?

A: The benefits derived from consuming mushroom extracts depends on the kind of mushroom consumed. For example: Lion’s Mane mushrooms are known for their cognitive and neuroprotective properties, Cordyceps mushrooms are great for enhancing athletic performance ad Reishi mushrooms provide stress relief and immune system support.

References

Suzanne Nielsen “Phenol-Sulfuric Acid Method for Total Carbohydrates” Food analysis laboratory manual (2009) doi: 10.1007/978-1-4419-1463-7_6

Danielson et. al. “Enzymatic Method To Measure β-1,3-β-1,6-Glucan Content in Extracts and Formulated Products (GEM Assay)” Journal of Agricultural and Food Chemistry (2010) doi: 10.1021/jf102003m

Ko and Lin “1,3-β-Glucan Quantification by a Fluorescence Microassay and Analysis of Its Distribution in Foods” Journal of Agricultural and Food Chemistry (2004) doi: 10.1021/jf0354085

Liang et al “The extract optimization and identification study of bioactive total triterpenoids from the rare traditional Chinese medicine Qinling Polyporusumbellatus” Journal of Chemical and Pharmaceutical Research (2014)

“Fungal ITS Sequencing“. CD Genomics

“Fungal sequencing and classification with the ITS Metagenomics Protocol“. Illumina

“Sanger Sequencing: Introduction, Principle, and Protocol“. CD Genomics

Perez et al “The Chemistry Behind the Folin–Ciocalteu Method for the Estimation of (Poly)phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern“. Journal of Agriculture Food Chemistry (2023) doi: 10.1021/acs.jafc.3c04022

“ICP-MS Heavy Metal Analysis“. Pacific Biolabs

Cortina-Escribono et. al. “Effect of Strain, Wood Substrate and Cold Treatment on the Yield and β-Glucan Content of Ganoderma lucidum Fruiting Bodies“. Molecules (2020) doi: 10.3390/molecules25204732

Mehta et. al. “Effect of cost-effective substrates on growth cycle and yield of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (higher Basidiomycetes) from Northwestern Himalaya (India)“. Integrated Journal of Medicinal Mushrooms doi: 10.1615/intjmedmushrooms.v16.i6.80.

 

What is Lion’s Mane?

Lion’s Mane Mushroom—also known as Hericium erinaceus in Latin, Monkey Head mushroom in China, Yamabushitake in Japan, Khargosh Chewn in Northern India, and even Roe Deer Butt mushroom in Korea—has a fascinating array of names across the globe. If you’re new to the world of mushrooms, you’ll quickly notice a trend: mushrooms are often named based on their appearance or the trees they grow on. And indeed, names like Lion’s Mane, Rabbit Fur, Roe Deer Butt, and even Monkey’s Head are pretty fitting for this unique, fluffy mushroom.

But here’s a quirky twist: Have you ever wondered why the Japanese named this mushroom “Yamabushitake” (山伏茸)? This name comes from the Yamabushi monks—ascetic hermits who live in the mountains. The name “Yamabushi” literally translates to “one who prostrates oneself on the mountain,” and it’s not just about the looks! These monks used Lion’s Mane mushrooms to boost their brain activity during meditation, believing they held cognitive, spiritual, and healing properties. Pretty cool, right?

In fact, the Yamabushi monks were onto something far ahead of their time. Over the last 40 years, Lion’s Mane, or Hericium erinaceus, has sparked over 400 scientific publications, all trying to understand this mushroom’s therapeutic potential. So what does the research say? Let’s dive into the science of this magical mushroom!

Before diving into the benefits, if you’d like to gain a deeper understanding of Lion’s Mane mushrooms—including how they grow, their geographic distribution, cultivation techniques, and the unique compounds they contain—we recommend checking out this detailed blog first. It’s the perfect starting point to explore everything about this incredible mushroom and how it grows!

https://nuvedo.com/2024/03/12/unlocking-the-secrets-of-lions-mane-mushroom-extracts-a-comprehensive-beginners-guide/?srsltid=AfmBOorzDSPGAjWxx-hP7dBqNfwC5rgR1hf3p24P9EBpkLBcv64oozHb

 

A Peek Into the Science of Lion’s Mane

A quick search on Google Scholar for “Hericium erinaceus” brings up about 17,000 results. Okay, Google Scholar is great, but let’s be honest—PubMed is where the cool kids go for medical research. And guess what? There are over 420 studies on Lion’s Mane benefits there!

Now, hold on! You’re probably thinking, “That’s fantastic, but what about the human clinical trials?” Well, here’s the fun part: We’ve got 9 human clinical trials to date. The first one, way back in 1985, involved 50 participants who received Lion’s Mane mushroom to treat chronic gastritis. Fast forward to 2023, and the most recent study was the first to look at healthy participants using Lions mushroom. In total, over 400 participants have been part of these trials!

A particularly interesting trend is that the majority of research on Lion’s Mane benefits has been published between 2019–2023, with over 60% of the studies coming out in just those 4 years. It’s clear that the focus is on cognitive health and mental well-being, which is why Lion’s Mane continues to capture the attention of researchers.

While the research is still developing, the evidence so far is promising! The best part? It’s safe to consume without any reported adverse effects.

What Are the Benefits of Lion’s Mane Mushroom?

The main benefits of Lion’s Mane include its ability to enhance cognitive health, support gut healing, reduce stress, and naturally boost focus. So, what makes Lion’s Mane mushroom so special? There’s a mountain of research to back its benefits, but here’s a fun rundown of the top 7 reasons to start incorporating Lion’s Mane into your life (besides the fact that it’s just awesome!).

7 Reasons to Add Lion’s Mane Mushroom to Your Wellness Routine

• Protect Your Brain from Alzheimer’s Disease

• Fight Depression and Anxiety Naturally

• Supercharge Your Morning Coffee

• Manage Stress Like a Pro

• Enhance Focus and Mental Performance

• Improve Gut Health and Digestion

• Support Myelin Growth and Help ADHD Symptoms

Let’s dive deeper into each benefit and explore the research that supports these claims!

1. Protect Your Brain from Alzheimer’s Disease

As the world’s population ages, we’re seeing a rise in neurodegenerative diseases like Alzheimer’s disease (AD), which is now the fifth-leading cause of death in adults over 65. Alzheimer’s is associated with the buildup of amyloid plaques in the brain, which damage neurons and disrupt brain function.

Here’s where Lion’s Mane mushroom advantages come into play: A 2016 study showed that Lion’s Mane extract reduced amyloid plaques by 40% in mice with Alzheimer ’s-like brain physiology. Plus, it enhanced enzymes that help clear harmful proteins and improved behaviors related to memory and daily tasks.

But don’t just take our word for it—there have been 3 human clinical trials that showed Lion’s Mane supplementation helped improve cognitive function in people with Alzheimer’s. These improvements seem to be temporary, though, which suggests that long-term use is key for sustained benefits. Still, the evidence points to this mushroom having a promising role in Alzheimer’s prevention and management.

2. Fight Depression and Anxiety Naturally

Ever heard of NGF (nerve growth factor) and BDNF (brain-derived neurotrophic factor)? These two proteins are like the superheroes of brain health. They help your neurons stay healthy, repair damaged cells, and even create new brain cells. Sadly, low levels of NGF and BDNF are often linked to depression and anxiety.

This is where Lion’s Mane shows off its magic. It’s rich in terpenes (specifically erinacines and hericenones) that can stimulate the production of NGF and BDNF in your brain. This might explain why Lion’s Mane has antidepressant effects.

In a 2010 study, people who took Lion’s Mane showed a 26% improvement in mood and a 36% decrease in anxiety in just 4 weeks. The placebo group didn’t see much of a change, which suggests Lion’s Mane has something special to offer.

3. Supercharge Your Morning Coffee

 

Do you love your morning coffee but wish it could give you more than just a caffeine jolt? Lion’s Mane and Coffee might just be the ultimate power couple.

 According to this 2023 study which compared Guayusa extract (which contains caffiene) lions mane extract and placebo– adding Lion’s Mane to your routine could offer some notable benefits over caffeine (from Guayusa). Participants who took Lion’s Mane showed a 28% greater improvement in focus at 120 minutes compared to those who had caffeine, with similar improvements seen at 60 minutes. In terms of mood, Lion’s Mane outperformed Guayusa with a 10% greater improvement in happiness at 120 minutes and a 7% improvement at 60 minutes. For productivity, Lion’s Mane showed a 35% increase in productivity at 60 minutes and a 30% improvement at 120 minutes compared to Guayusa. These results suggest that Lion’s Mane not only boosts focus but also reduces reaction time, enhances mood and productivity, providing longer-lasting benefits than the short-term energy boost from caffeine. So, if you’re looking for sustained cognitive performance, replacing or adding Lion’s Mane to your coffee could be a great choice.

It turns out, that Lion’s Mane isn’t just good for focus—it enhances mood and productivity, offering long-lasting benefits without the crash that comes with caffeine.

4. Manage Stress Like a Pro

Life can get pretty stressful, right? Whether it’s work, school, or home life, stress often feels like a constant companion. But here’s some good news: Lion’s Mane can help reduce stress levels naturally. A recent 28-day study on healthy adults aged 18–45 found that taking Lion’s Mane led to a 22.5% reduction in perceived stress, with participants’ stress levels dropping from a mean score of 40.64 on Day 1 to 33.02 on Day 29. In comparison, the placebo group showed no significant change (mean score of 42.53 on Day 29). If you’re looking for a natural way to de-stress, Lion’s Mane might just be your new best friend!

 

5. Enhance Focus and Mental Performance

Ever wished for a magic pill to boost your brainpower? Lion’s Mane might be the closest thing to it. A 2019 study on overweight and obese adults found that Lion’s Mane supplementation led to remarkable improvements in memory, focus, mood, and overall quality of life. Cognitive performance jumped by 23%, memory recall improved by 25%, and attention and focus were 4 times better than the control group. Participants reported feeling 10 times clearer and more uplifted, with mood and mental clarity improving by 20%, while their overall quality of life saw a 5-fold boost. If you’re looking for sustained cognitive benefits, Lion’s Mane may be the brain-enhancing ally you’ve been waiting for!

6. Improve Gut Health and Digestion

Lion’s Mane isn’t just great for the brain; it also supports gut health—a key factor in overall well-being. Some of the earliest research on this mushroom involved its potential to help with digestive issues.

One pivotal study from 1985 investigated the use of Lion’s Mane for treating chronic atrophic gastritis (a condition where the stomach lining becomes inflamed and damaged). Participants who took Lion’s Mane extract reported significant improvements in their condition, and many saw a reduction in stomach pain, bloating, and acidity. This suggests that Lion’s Mane may have the ability to help heal the stomach lining and balance gastric acid levels, making it a useful ally for people with gastritis, ulcers, or even acid reflux.

Lion’s Mane’s potential for gut healing doesn’t stop there. Research also indicates that it promotes the growth of beneficial gut bacteria, which plays a role in maintaining a healthy digestive system. So, if you struggle with digestive discomfort or acidity, incorporating Lion’s Mane could help restore balance in your gut and bring some much-needed relief.

 

7. Support Myelin Growth and Help ADHD Symptoms

Have you ever wondered why people with ADHD often struggle with focus or impulse control? The answer might lie in the insulation around their nerve cells—yes, just like how wires need insulation to function properly!

That’s where myelin comes in. This fatty, protein-rich substance acts as a protective sheath around your nerve cells, allowing brain signals to travel quickly and efficiently. Without myelin, your brain’s communication system is like a slow, interrupted Wi-Fi connection.

Here’s how myelin works:

• Insulation: Myelin shields your nerve cells, much like how electrical cables are wrapped in a protective covering.
  
• Speed: It accelerates the transmission of signals by allowing electrical impulses to jump between “nodes” (think of them as stepping stones), speeding up communication.
  
• Strength: It helps maintain the strength of these signals as they travel through your brain. Without myelin, signals can weaken, leading to neurological problems.

Now, what does this have to do with ADHD? Well, a disruption in myelination (the process of building myelin) could be a key factor in the development of ADHD. If the myelin sheath isn’t functioning properly, it can impair communication between brain cells, which may contribute to cognitive challenges, difficulty focusing, and other ADHD symptoms.

Here’s the good news: Our magical mushroom Lion’s Mane may offer a solution! A 2003 study showed that Lion’s Mane extract could help promote myelin production. After 26 days of treatment, nerve cells treated with Lion’s Mane had an average of 10.03 layers of myelin, compared to just 8.07 layers in untreated cells—an impressive 24% increase.

So, if you or someone you know is dealing with ADHD symptoms, Lion’s Mane might offer a natural boost to the myelin-making process, potentially improving brain function and communication.

 

 

Wrapping Up: Why Lion’s Mane Mushroom Is a Must-Have

Lion’s Mane is a powerhouse mushroom packed with benefits for brain health, mental well-being, and overall performance. Whether you’re looking to boost your cognitive function, fight stress, or improve gut health, Lion’s Mane has you covered. With an ever-growing body of research and centuries of traditional use, it’s clear this mushroom is far from a passing trend.

 

Choosing Supplements: What makes some Lion’s Mane supplements better than others?

When deciding on a Lion’s Mane supplement, understanding the differences between Lion’s mane mushroom powder and extracts can help you choose the most effective option.

Lion’s Mane Mushroom Powder

• Composition: Made by drying and grinding the fruiting body, this is a whole-food option containing a range of nutrients.
• Terpenes and Potency: While it includes polysaccharides, the concentration of terpenes like hericenones and erinacines—key compounds for brain and nerve health—is lower compared to extracts.
• Bioavailability: Without breaking down the tough chitin in mushroom cell walls, much of its nutritional value remains locked, limiting absorption.

Lion’s Mane Dual Extract

• Concentrated Potency: Extracts are created using a dual process that isolates both water-soluble polysaccharides and alcohol-soluble terpenes, significantly enhancing potency.
• Terpenes and Bioavailability: The process makes compounds more bioavailable, ensuring maximum benefits for cognitive and nerve health.

At Nuvedo, We Go Beyond Standard Practices to Deliver a Lion’s Mane Extract That Redefines Quality

1. Patent-Pending Ultrasound-Assisted Extraction

   • Our revolutionary process ensures the maximum extraction of bioactive compounds, including both water-soluble polysaccharides and alcohol-soluble terpenes, for unparalleled efficacy.

2. From Spore to Extract

   • We oversee every step, from cultivating pure fungal cultures to growing mushrooms and performing the final extraction, ensuring complete traceability, consistency, and quality.

3. High Potency & Purity

   • With superior bioavailability and concentration, our extracts provide the full spectrum of Lion’s Mane’s therapeutic benefits, supporting cognitive and nerve health like no other.

4. Tested for Safety and Potency

   • Each batch undergoes rigorous testing for quality, purity, and safety. We are proud to hold ISO and HACCP certifications, ensuring you get only the safest and most effective product.

5. Taste and Experience

   • Our unique process minimizes ethanol content, delivering a smooth, earthy flavor that lets the true taste of Lion’s Mane shine through.

6. Comprehensive Options for All Needs

   • Whether you want a high-potency extract or a natural option, we’ve got you covered. We also offer dehydrated fruitbodies for those who prefer the versatility of using whole mushrooms in their recipes or teas.

The Nuvedo Promise

With Nuvedo, you’re not just choosing a supplement—you’re embracing a product born of passion, innovation, and an unwavering commitment to quality. No matter which form you prefer, our Lion’s Mane products are crafted to help you unlock your best self. 🌿

What do you think? 😊

 

FAQs

• What is Lion’s Mane Mushroom Good For?

         Lion’s Mane is known for:

• Boosting brain function: Improves memory, focus, and cognitive health.
• Supporting nerve health: Helps repair and grow nerve cells.
• Enhancing gut health: Reduces inflammation and supports a healthy microbiome.
• Boosting immunity: Modulates the immune system.
• Mental wellness: Reduces anxiety and depression symptoms.

• Can Lion’s Mane Help with ADHD?

Yes, Lion’s Mane may help with ADHD by:

• Improving focus: Supports brain health and attention.
• Promoting remyelination: Helps repair nerve fibers, which improves brain communication.
• Reducing inflammation: Calms brain inflammation, which can improve symptoms.

• How Does Lion’s Mane Improve Gut Health?

Lion’s Mane improves gut health by:

• Repairing the gut lining: Reduces inflammation and supports gut healing.
• Supporting the microbiome: Encourages healthy gut bacteria growth.
• Improving the gut-brain connection: Promotes balance between digestion and mental health.

 

Want to learn more? Check out the references and dive deeper into the world of Lion’s Mane:

1. ADHD and Myelination
   https://pubmed.ncbi.nlm.nih.gov/30786021/
   
2. Mushroom Lion’s Mane and Myelination
   https://fz.kiev.ua/journals/2003_V.49/Fiziologichnyi%20Zhurnal%2049(1)_2003/Fiziologichnyi%20Zhurnal%2049(1)_2003_38-45.pdf
   https://www.nature.com/articles/s41598-021-85972-2
   
3. Mushroom Lion’s Mane and Stress Reduction + Reaction Time
   https://pmc.ncbi.nlm.nih.gov/articles/PMC10675414/
   
4. Lion Mane mushroom and Depression + Anxiety
   https://pubmed.ncbi.nlm.nih.gov/20834180/
   
5. Lion Mane mushroom and Amyloid Plaque Buildup
   https://link.springer.com/article/10.1186/s12929-016-0266-z
   
6. Lion Mane mushroom and Alzheimer’s
   https://pmc.ncbi.nlm.nih.gov/articles/PMC9331832/
   
7. Role of BDNF & NGF Levels in Depression
   https://www.tandfonline.com/doi/figure/10.1080/00207454.2018.1527328?scroll=top&needAccess=true
   
8. Lion Mane mushroom and NGF/BDNF
   https://www.tandfonline.com/doi/full/10.1080/21501201003735556#d1e1157
   https://www.jstage.jst.go.jp/article/bpb/31/9/31_9_1727/_article/-char/ja/
   
9. Lion Mane mushroom and Cognition
   https://www.jstage.jst.go.jp/article/biomedres/40/4/40_125/_article
   https://pmc.ncbi.nlm.nih.gov/articles/PMC6500611/
   
10. Lion Mane mushroom and Gastritis
    https://mednexus.org/doi/epdf/10.5555/cmj.0366-6999.98.06.p455.01

Discovering the Delightful Shiitake Mushroom: A Comprehensive Exploration

In the realm of culinary and medicinal mushrooms, few varieties are as revered as the Shiitake mushroom (Lentinula edodes). It has a rich umami flavor which makes it versatile for culinary applications. It also boasts a plethora of health benefits. This unique combination of taste and health has earned the Shiitake mushroom a special place in cuisines and traditional medicines around the world. In this article, we uncover the mysteries surrounding this esteemed fungus, exploring its origins, cultivation methods, health benefits, culinary uses, and more.

Figure: Shiitake mushroom (Lentinula edodes) (image)

What is Shiitake Mushroom?

The Shiitake mushroom’s scientific name is Lentinula edodes. It is a flavorful and nutritious fungus native to East Asia, particularly Japan, China, and Korea. Its name is derived from the Japanese words “shii” (Castanopsis cuspidata a type of hardwood tree ) and “take” (mushroom), as it is commonly found growing on dead or decaying hardwood logs of this tree. It is also known as xiang-gu in China and p’yogo in Korea. 

Figure: Castanopsis cuspidata, also known as Shii in Japan, is the traditional host for shiitake mushrooms (image)

Physical Characteristics:

Shiitake mushrooms typically have broad, umbrella-shaped caps that range in color from light to dark brown. The caps are often covered with small, white flecks or contain white cracks which add to the aesthetic appeal of the mushroom. The underside of the cap features gills that release spores for reproduction.The stipe of shiitake mushrooms is quite tough and is used to make powders or broths and soups.

Taxonomy and Classification:

Shiitake mushrooms belong to the Lentinula genus within the order Agaricales. Evolutionarily, the most common ancestor of modern shiitake mushrooms emerged 28.3 million years ago. These then split into 4 groups with 3 groups primarily colonising sub-tropical habitats of the Americas and one group colonsing parts of Asia and Australasia. 

The genus Lentinula can be divided into 13 species distributed globally. Of the 13 species, Lentinula edodes sensu lato, native to Japan, China, Korea and Eastern Russia represent what is traditionally cultivated as Shiitake mushrooms.

a)                                                                                                    b )

        

c)                                                                                                   d)

         

 Figure: Lentinula lateritia (image), Lentinula raphanica (image), Lentinula novae-zealandiae (image), Lentinula aciculospora (image)

Geographic Distribution:

The most commonly consumed shiitake mushroom species originated from Eastern Asia in parts of China, Japan and Korea. Other subspecies of this variety have also been found in parts of Thailand, Vietnam and Nepal. 

Other members of the genus have been found in Australia, New Zealand, Costa Rica, USA, parts of Africa and even Madagascar.

While native to East Asia, Shiitake mushrooms are now cultivated and consumed worldwide. They thrive in temperate regions with mild winters and moderate rainfall, making them suitable for cultivation in diverse environments.

Figure: Geographic distribution of different species belonging to the genus Lentinula. Notice how Lentinula edodes (Shiitake mushrooms) are primarily found in parts of Eastern and Southeast Asia (image)

Cultivation Regions:

Major Shiitake-producing countries include Japan, China, Korea and the United States. Each region has its own cultivation methods, traditions, and culinary uses for Shiitake mushrooms, contributing to the mushroom’s global appeal and cultural significance.

Figure: Traditional log cultivation practices of Japan (image)

 

 

Figure: Modern shiitake mushroom cultivation in China (image)

Ecological Role:

Shiitake mushrooms are white rot fungi that are primary decomposers of dead wood in forest habitats. They do not traditionally exhibit parasitic behaviours and feed mainly on fallen logs. They can break down cellulose, hemicellulose and lignin making them essential to the forest carbon cycle.

 

Figure: Shiitake mushrooms growing out of a fallen log in a forest (image)

Historic Use and Cultural Significance:

Shiitake mushrooms have a long history of culinary and medicinal use in East Asian cultures, where they are prized for their flavor, texture, and potential health benefits. They hold a special place in traditional cuisines, rituals, and festivals, symbolizing prosperity, longevity, and good fortune.

Culinary Tradition:

In Japanese, Chinese, and Korean cuisines, Shiitake mushrooms are featured in a wide range of dishes, including soups, stir-fries, steamed dishes, and sushi. They are valued for their meaty texture and savory flavor, which adds depth and complexity to traditional recipes.

 

a)                                                                                             b)

                                               

 

c)                                                                                                   d)

                   

 

Figure: Traditional dishes prepared using shiitake mushrooms: a) Braised mushroom with Bok Choy (image) b) Shiitake mushroom Shoyuzuke (soy sauce pickled shiitake mushrooms) (image) c) Shiitake mushroom infused dashi (traditional japanese broth) (image) d) Stir-fried Asian greens with shiitake mushroom (image)

Medicinal Uses:

In traditional Chinese medicine (TCM), Shiitake mushrooms are believed to have various health-promoting properties, including immune modulation, cardiovascular support, and anti-inflammatory effects. They are often used as a tonic for vitality and longevity, consumed as a food or herbal remedy.

Symbolism and Festivals:

Shiitake mushrooms are associated with prosperity and good fortune in East Asian cultures. They are often served during festive occasions such as weddings, New Year celebrations, and harvest festivals.  

 

                                                 

Figure: Shiitake mushroom gift boxes given during major festivals such as Chinese new year (image)(image)

Cultivation History and Methods:

The cultivation of Shiitake mushrooms dates back centuries, with records of its cultivation in East Asia as early as the 12th century. Over time, traditional cultivation methods have evolved into modern techniques that maximize yield, quality, and sustainability.

Historical Cultivation:

The use of shiitake mushrooms dates back to ancient China, during the Sung dynasty in 1100 AD. The discovery of its culinary potential is credited to Wu Sang Kang who noticed these mushrooms growing on fallen logs in the forest. He would cut logs with shiitake mushrooms and bring them into the town for further harvesting.

From China, the practice of consumption of shiitake mushrooms spread further east to Korea and Japan. Japan was the first country to formalize the cultivation of Shiitake mushrooms. The use of shiitake mushrooms in Japan dates back several centuries. Initially, they were mostly foraged from the wild and from fallen trees. These logs were transported from the forests to town squares and courtyards where they would be regularly harvested.

It wasn’t until 1943 when a Japanese student of agriculture from Kyoto University, Kisaku Mori, developed the ‘Mori Method’ of cultivation allowing shiitake cultivation to really take off. In this method, small plugs of sterilised wood chips were inoculated with shiitake mycelium and left to colonise. Once covered in shiitake mycelium, the wood chips were used to inoculate holes in freshly cut tree logs and left to colonise.

Figure: Shiitake mushroom plug spawn made by inoculating mushroommycelium into compressed wood pellets (image)

Modern Cultivation Techniques:

In modern Shiitake cultivation, logs are often replaced with artificial substrates such as sawdust, straw, or supplemented hardwood chips. Being a wood-loving species, sawdust or wood chips supplemented with bran are optimum for shiitake cultivation.  

Sterilised substrate is inoculated with mushroom spawn and incubated in climate-controlled environments until fully colonized. Once colonized, the colonised blocks are exposed to conditions that trigger fruiting, such as changes in temperature, humidity, and light.

Sustainable Cultivation Practices:

As interest in sustainable agriculture grows, there has been a shift towards organic and environmentally friendly cultivation practices for Shiitake mushrooms. This includes sourcing local, sustainably harvested substrates, minimizing waste through recycling and composting, and reducing energy consumption through efficient heating and cooling systems.

Health Benefits:

Shiitake mushrooms are renowned for their potential health benefits, attributed to their rich nutritional profile and bioactive compounds. From immune support to cardiovascular health, these mushrooms offer a wide range of therapeutic properties backed by scientific research.

 

Immune Support:

Shiitake mushrooms contain polysaccharides such as beta-glucans, which have been shown to stimulate immune cell activity and enhance the body’s defense against infections and diseases. These compounds may help modulate immune responses and promote overall immune system health.

 

Cardiovascular Health:

Studies suggest that Shiitake mushrooms may have beneficial effects on cardiovascular health, including lowering cholesterol levels, reducing inflammation, and improving blood circulation. Compounds such as eritadenine and beta-glucans are believed to contribute to these cardioprotective effects.

 

Antioxidant Properties:

Shiitake mushrooms are rich in antioxidants such as selenium, vitamin C, and polyphenols, which help neutralize harmful free radicals and protect cells from oxidative damage. Regular consumption of Shiitake mushrooms may help reduce the risk of chronic diseases and support overall health and well-being.

 

Skin Care:

Shiitake mushrooms are rich in B vitamins and copper, which are important for the maintenance of healthy skin. Copper stimulates collagen production and can reduce the appearance of wrinkles. Shiitake mushrooms are also a great source of hyaluronic acid. Hyaluronic acid is a great natural moisturising agent which prevents wrinkles and makes the skin look more youthful. Hyaluronic acid and collagen together aid in the anti-aging effects shiitake mushroom extracts have on skin.

 

A rather unique component of shiitake mushroom extracts is Kojic acid which promotes fairness by inhibiting the production of melanin pigment in skin cells. Moreover, the antioxidants and polyphenols present in shiitake mushrooms protect the cells of the skin and reduce oxidative damage. These diverse properties have made the use of shiitake mushroom extracts in cosmetics mainstream.  

 

Bioactive Molecules

Polysaccharides

Polysaccharides are derived from the cell walls of shiitake mushrooms. They are a great source of dietary fiber and have been shown to be effective in reducing symptoms of colitis in animal models. They also possess anti-inflammatory, antioxidant and anti-bacterial properties.

Lentinan

Lentinan is a beta glucan isolated from Shiitake mushrooms. It is well known for its immunomodulatory properties. It has been shown to have anticancer, antiviral, antibacterial and hepatoprotective effects.

 

Lentinan is one of the most widely studied mushroom beta glucans and a number of clinical trials have been performed using this molecule. It has been regularly used to enhance cancer therapy in China and Japan and has been tested on patients suffering from lung cancers, gastric cancers, colorectal cancers, gynacologic cancers and liver cancers to name a few.

 

 

Figure: Structure of lentinan, a beta glucan molecule present in shiitake mushrooms (image)

Eritadenine

Eritadenine is an adenine derivative found in alcohol extracts of Shiitake mushrooms. It has potent cardioprotective effects. It has been shown to reduce serum cholesterol, triglyceride and phospholipid levels. Through this action it helps maintain a healthy cardiovascular system and prevent heart disease. In rat studies it was shown to be ten times more potent than Clofibrate, a cholesterol controlling drug (Sugiyama et. al.). 

 

Figure: Structure of eritadenine (image)

Lenthionine

Lenthionene is a sulfur containing cyclic molecule found in Shiitake mushrooms. It is a major flavour compound of shiitake mushrooms. Apart from flavour it also exhibits anti-thrombotic properties, meaning it can prevent blood clots. This is another method by which shiitake mushrooms exhibit their cardioprotective properties. Prevention of blood clots can significantly reduce the chances of heart attacks. 

 

Figure: Structure of lenthionine (image)

Carvacrol

Carvacrol is a monoterpenoid compound found in shiitake mushroom essential oils. It is also found in other plants such as oregano, thyme and bergamot. It has potent antibacterial, antifungal and neuromodulatory properties. It also acts as an antioxidant.

 

 

Figure: Structure of carvacrol (image)

How to consume shiitake mushrooms

Shiitake mushrooms are extremely versatile and can add strong umami flavours to any recipe. They are also potent medicinal wonders and therefore one may gain benefits beyond flavour from their consumption. Some of the common ways to incorporate shiitake mushrooms into your diet are:

Fresh

Fresh shiitake mushrooms are delicious and can be easily incorporated into asian style recipes. As they undergo minimal processing, they most closely represent the texture and flavour of the mushroom. However, due to their relatively low shelf life, they are hard to find in most places.

Figure: Fresh shiitake mushrooms (image)

Dried

Drying of shiitake mushrooms is the best way to preserve them for long term storage and intermittent use. Drying has enabled shiitake mushrooms to be available all over the world. The drying method plays an important role in the texture and flavour of the mushroom. Sun dried shiitake mushrooms even contain high levels of Vitamin D2.

Dried mushrooms have to be rehydrated in hot water before they can be used for cooking. This adds an additional step to the usage of these mushrooms. The water used for rehydration is traditionally used up in the cooking process as it contains flavour molecules as well.

 

Figure: Dried shiitake mushrooms (image)

Powders

Powders are made by grinding dried mushrooms. They are a great way to incorporate the mushrooms into soups, stocks and broths without having large chunks of mushroom. They can also be packed into capsules or made into teas.

Figure: Powdered shiitake mushrooms (image)

Extracts

Extracts are the best way to obtain the medicinal benefits from shiitake mushrooms. Extracts contain concentrated bioactive molecules from fresh or dried mushrooms. For best results, dual extracts made from both water and alcohol are recommended. At Nuvedo we prepare our extracts using a novel ultrasound based technology to ensure high levels of alcohol soluble and water soluble molecules. 

 

The use of dual extracts guarantees that one reaps the maximum benefits from an extract. Water soluble molecules such as lentinan and hyaluronic acid promote a healthy cell behaviour while alcohol soluble molecules such as eritadenine maintain good cardiovascular health.

Figure: Our dual extracted Shiitake mushroom tincture offers the best of both worlds when it comes to functional benefits

 

Culinary Uses:

Shiitake mushrooms are prized for their rich, earthy flavor and meaty texture, making them a versatile ingredient in a wide range of culinary applications. From soups and stir-fries to sauces and marinades, these mushrooms add depth and complexity to dishes across various cuisines.

 

Stir-Fries and Sautees:

One of the most popular ways to enjoy Shiitake mushrooms is in stir-fries and sautéed dishes. Their robust flavor and firm texture make them ideal for quick-cooking methods, allowing them to absorb the flavors of other ingredients while retaining their own distinct taste.

 

Soups and Broths:

Shiitake mushrooms are a common ingredient in Asian soups and broths, adding depth of flavor and umami richness to the broth. Whether in miso soup, hot pot, or noodle soup, these mushrooms contribute a hearty, satisfying element to the dish.

 

Grilled or Roasted:

Grilling or roasting Shiitake mushrooms enhances their natural flavors and brings out their meaty texture. Simply marinate the mushrooms in olive oil, garlic, and herbs, then grill or roast until tender and caramelized

 

Conclusion

Shiitake mushrooms are a rare species having excellent culinary and medicinal potential. It may therefore come as no surprise that they are the second most widely cultivated mushroom around the world. Their beautiful appearance only adds to their allure. Regular consumption of shiitake mushrooms or shiitake mushroom extracts are a great way to ensure physical well-being, while enjoying some delicious meals. Unlike other mushroom extracts like Reishi or Cordyceps extracts, Shiitake mushroom extracts can be consumed at ay time of the day.

The benefits from the application or consumption of shiitake mushrooms are only visible over a few months. If taken regularly, shiitake mushrooms are a safe way to promote longevity and wellbeing without any side effects. Shiitake mushrooms can supplement any diet or exercise regimen and restore balance to the body.

References

Mizuno “Shiitake, lentinus edodes: Functional properties for medicinal and food purposes” Food Reviews International (1995)

Zhang et. al. “Advances in lentinan: Isolation, structure, chain conformation and bioactivities” Food Hydrocolloids (2011)

Zhang et. al. “Mushroom polysaccharide lentinan for treating different types of cancers: A review of 12 years clinical studies in China” Progress in Molecualr Biology and Translational Science (2019)

Rao “Extraction, purification, bioactivities and prospect of lentinan: A review” Biocatalysis and Agricultural Biotechnology (2021)

Sugiyama “Hypocholesterolemic Action of Eritadenine Is Mediated by a Modification of Hepatic Phospholipid Metabolism in Rats” The Journal of Nutrition (1995)

Takashima “The hypocholesterolemic action of eritadenine in the rat” Atherosclerosis (1973)

Sierra-Patev et. al. “A global phylogenomic analysis of the shiitake genus Lentinula” PNAS (2023)

Roszczyk et. al. “Immunomodulatory Properties of Polysaccharides from Lentinula edodes” International Journal of Molecular Sciences (2022)

C.A. Alagbaoso “Polysaccharides from Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) Suppress pMLKL-Mediated Necroptotic Cell Death and Colitis in Mice” International Journal of Medicinal Mushrooms (2021)

In the realm of fungi, few specimens command as much awe and reverence as the Lion’s Mane mushroom (Hericium erinaceus). Its unique appearance, coupled with a rich history of medicinal and culinary use, has cemented its status as a revered organism. In this article, we embark on a journey to unravel the mysteries surrounding this remarkable fungus, delving into its origins, cultivation, health benefits, and more.

What is Lion’s Mane Mushroom?

Lion’s Mane mushroom is scientifically known as Hericium erinaceus. Its spore bearing projections cascade down the fruiting body resembling a long mane, earning it many names. It is commonly known as llion’s mane mushroom, but also goes by bearded tooth fungus, pom pom mushroom and monkey head mushroom. 

Physical Characteristics:

The most distinctive features of lion’s mane mushrooms are their beard like appearance. It belongs to a group of fungi called hydnoid or tooth fungi. All members of this family bear their spores on spine-like projections which protrude from the basidiocarp or fruiting body. Other members of this group include the beautiful mealy tooth mushroom and the toothed jelly mushroom.

Lion’s mane mushrooms coloration ranges from creamy white to pale yellow. They are generally found growing on hardwood trees and are saprobic as well as parasitic in nature.  Just like all other mushrooms, lion’s mane mushrooms propagate using spores borne on their teeth. 

Geographic Distribution:

Lion’s Mane mushroom is native to temperate regions of North America and Asia. Most notably, they are found in the alpine forests of Japan, China, Korea and the Pacific Northwestern region of North America. Recently a closely related species named Hericium bharengense was discovered in Sikkim.

They thrive in moist, woodland habitats with temperatures below 25℃. It is commonly found growing on dead or decaying hardwood logs, stumps, and branches, particularly those of oak, beech, and maple trees.

Ecological Role:

In its natural habitat, Lion’s Mane mushroom plays a vital ecological role as a decomposer, breaking down dead or dying wood and recycling nutrients back into the soil. Its ability to colonize hardwood trees makes it a key player in forest nutrient cycling and ecosystem health.

Historic Use and Cultural Significance:

The use of Lion’s Mane mushroom dates back centuries, with records of its consumption for both culinary and medicinal purposes in traditional Chinese medicine and Japanese cuisine. Traditionally known as Yamabushitake (Japanese) or Hou you gu (Mandarin Chinese), it is revered for its potential cognitive and digestive health benefits.

Traditional Medicine:

In traditional Chinese medicine (TCM), Lion’s Mane mushroom has been used for centuries to treat a variety of ailments, including digestive disorders, inflammation, and cognitive decline. It is believed to have a cooling, nourishing effect on the body, promoting balance and vitality.

Culinary Tradition:

In addition to its medicinal properties, Lion’s Mane mushroom has a long history of culinary use in Asian cuisine, particularly in Japan and China. It is prized for its delicate flavor and unique texture, often featured in soups, stir-fries, and vegetarian dishes.

Symbolism and Folklore:

Lion’s Mane mushroom holds a special place in the folklore and symbolism of many cultures, often representing wisdom, strength, and longevity. In Japanese culture, it is associated with the mythical creature “Shishi,” symbolizing protection and good fortune.

Cultivation History and Methods:

Lion’s mane mushrooms were traditionally harvested from the wild. The cultivation of Lion’s Mane mushroom has gained popularity in recent years due to its increasing demand. Cultivation typically involves inoculating sterilized substrates such as sawdust, straw, or logs with mushroom spawn, providing optimal conditions for growth, including humidity, temperature, and ventilation.

Historical Cultivation:

The cultivation of Lion’s Mane mushroom dates back centuries, with records of its cultivation in China as early as the 15th century. Traditional cultivation methods involved inoculating logs or straw beds with mushroom spawn and maintaining optimal environmental conditions for fruiting.

Modern Cultivation Techniques:

In modern times, the cultivation of Lion’s Mane mushroom has been streamlined and optimized through advances in technology. Commercial growers use techniques such as controlled environment cultivation, automated irrigation systems, and sterile laboratory procedures to maximize yield and quality.

Ready-to-fruit Grow Kits

One of the best ways to obtain fresh Lion’s mane mushroom fruiting bodies is to use ready-to -fruit grow kits. These kits are made of colonised substrate packed into a box format. All one has to do is spray some water a couple of times a day and fresh mushrooms grow out within 3 weeks.

Sustainable Cultivation Practices:

As interest in sustainable agriculture grows, there has been a renewed focus on sustainable cultivation practices for Lion’s Mane mushroom. This includes sourcing local, organic substrates, minimizing waste through composting, and reducing energy consumption through efficient heating and cooling systems.

Health Benefits:

Lion’s Mane mushroom is primarily associated with cognitive enhancement and neuroprotective effects. However it also has significant effects on digestive health and immune system modulation

Cognitive Enhancement:

Lion’s Mane mushrooms are widely researched for their ability to enhance cognitive functions. The consumption of the mushroom in the form of extracts or powders can improve focus and mental clarity.

Brain Support

Compounds in mion’s mane mycelia and fruiting bodies such as hericenones and erinacines stimulate the production of nerve growth factor (NGF) and promote neuronal growth and repair. This keeps the brain in a state of good health and prevents neurodegeneration.

Stave off Alzheimer’s Disease

Due to the neuroprotective effects of compounds in Lion’s mane extracts, many clinical trials have been conducted to investigate the use of lion’s mane for the management of Alzheimer’s disease. Some studies report improvements in cognitive function compared to the placebo treated group. The promotion of NGF secretion also helps delay the onset of symptoms and slow down disease progression

Digestive Health Support:

Lion’s Mane mushrooms promote digestive health by supporting the growth of beneficial gut bacteria and reducing inflammation in the digestive tract. This can help alleviate symptoms of gastrointestinal disorders such as inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS).

Immune System Modulation:

Polysaccharides such as beta glucans from lion’s mane mushrooms enhance immune function, helping the body defend against infections and diseases. These compounds stimulate immune cell activity and modulate inflammatory responses, contributing to overall immune system health.

Improved Fat Metabolism

Extracts of lion’s mane mushrooms promote fat metabolism in mouse studies. Both hot water and ethanol extracts when given to mice fed on a high fat diet, resulted in a much lower weight gain when compared to placebo controls.

Bioactive Molecules:

Lion’s Mane mushroom contains a diverse range of bioactive molecules, each with its own unique properties and potential health benefits. Some of the key bioactive compounds found in Lion’s Mane mushroom include hericenones, erinacines, polysaccharides, terpenoids, and phenolic compounds.

Hericenones 

Hericenones are diterpenoid compounds found in lion’s mane mushrooms which stimulate the secretion of nerve growth factor (NGF) in the brain. They are found in the fruiting bodies of the mushroom and are soluble in ethanol.

Erinacines:

Erinacines are another group of diterpenoid compounds which have the ability to stimulate NGF in the brain. They are found in the mycelia of lion’s mane mushrooms and not in fruiting bodies. They are ethanol soluble in nature.

Hericene

Hericenes are monoterpenoid compounds isolated from fruiting bodies of lion’s mane mushroom. They have been shown to promote axonal outgrowths in hippocampal neurons and promote NGF secretion. They are also ethanol soluble in nature.

Polysaccharides:

Lion’s Mane mushroom contains a variety of polysaccharides which modulate immune function, act as antioxidants and may also have antimicrobial properties. They also support digestive health. Ploysaccharides are water soluble and can be found in extracts as well as dried mushrooms.

Beta glucans

Beta glucans are polysaccharides and are an essential component of fungal cell walls. They are responsible for the immuno-modulatory properties of lion’s mane mushrooms. They are water soluble compounds and can be found in hot water extracts or teas. 

Polyphenols

Polyphenols are the main antioxidant compounds in lion’s mane mushrooms and lion’s mane mushroom extracts. These compounds are extremely sensitive to heat so they are best consumed from dried mushrooms or extracts prepared at low temperatures. 

Methods of Consumption:

Lion’s Mane mushroom can be consumed in various forms, each offering its own unique culinary and nutritional benefits. Some popular methods of consuming Lion’s Mane mushroom include:

Fresh:

Fresh Lion’s Mane mushroom can be cooked in a variety of ways, including sautéing, grilling, or roasting. However the best way to retain the maximum bioactive molecules is  to minimise exposure to heat. Stir-frying is  a quick and easy way to retain the maximum antioxidant potential of these mushrooms.

Due to their low shelf life (unless they are dried), it is best to consume the mushrooms as close to harvesting them as possible. (Check out our Lion’s mane mushroom grow kits for the freshest lion’s mane mushrooms you can get)

Its delicate flavor and unique texture make it a versatile ingredient in savory dishes such as stir-fries, soups, and pasta dishes.

Dried:

Dried Lion’s Mane mushrooms can be rehydrated and used in cooking, or consumed as a supplement in the form of capsules or powder. Dried Lion’s Mane mushroom retains its nutritional value and can be easily stored for long periods. The drying temperature crucially impacts the amount of bioactive molecules retained. Sundried mushrooms or those dried at a low temperature retain the highest dose of bioactive molecules.

Extracts and Tinctures:

Lion’s Mane mushroom extracts and tinctures are a great way to consume concentrated forms of the mushroom’s active compounds. These extracts can be added to hot beverages such as tea or coffee, or taken directly as a supplement for cognitive support, digestive health, or immune system modulation. We prepare our Lion’s mane mushroom dual extracts using sound waves at low temperatures to retain the maximum number of bioactive molecules.

Tea:

Lion’s Mane mushroom tea is a popular beverage in traditional Chinese medicine, prized for its soothing flavor and potential health benefits. To make Lion’s Mane mushroom tea, simply steep dried mushroom slices or powder in hot water for several minutes, then strain and enjoy.

Coffee:

Due to their ability to improve focus and cognitive function, lions mane powders or extracts are often incorporated into coffee blends. The hit of caffeine and lion’s mane together provide a steady stimulation to do some focused work.

Clinical Trials:

Several clinical trials have been conducted to explore the therapeutic potential of Lion’s Mane mushroom, particularly in the realm of cognitive health and neuroprotection. While results have been promising, further research is needed to fully understand its mechanisms of action and therapeutic efficacy.

Cognitive Function:

Clinical trials investigating the effects of Lion’s Mane mushroom on cognitive function have shown promising results, with some studies reporting improvements in memory, attention, and overall cognitive performance in both healthy individuals and those with cognitive impairment. These effects are thought to be mediated by the mushroom’s ability to stimulate nerve growth factor (NGF) production and promote neuronal growth and repair.

Neurological Disorders:

Preliminary studies suggest that Lion’s Mane mushroom may have potential therapeutic benefits for neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. While more research is needed, early findings indicate that Lion’s Mane mushroom extracts may help protect against neuronal damage and promote neuronal regeneration in animal models of these diseases.

Gut Health:

Emerging research also suggests that Lion’s Mane mushroom may have beneficial effects on digestive health, including the treatment of inflammatory bowel diseases such as Crohn’s disease and ulcerative colitis. Clinical trials investigating the effects of Lion’s Mane mushroom supplements on gut inflammation and microbial balance are currently underway.

Conclusion:

In conclusion, Lion’s Mane mushroom stands as a testament to nature’s bounty, offering a plethora of health benefits and cultural significance. From its humble origins in the forest to its cultivation in modern laboratories, this remarkable fungus continues to captivate the imagination of scientists, chefs, and health enthusiasts alike. As research into its therapeutic properties expands, the Lion’s Mane mushroom is poised to reign supreme in the realm of natural remedies and culinary delights. Its rich history, diverse array of bioactive compounds, and potential therapeutic applications make it a true marvel of the natural world, worthy of exploration and appreciation.

 

Interested in incorporating Lion’s Mane extracts into your routine? Check out our selection of high-quality Lion’s Mane Extract: https://nuvedo.com/product/lions-mane-liquid-extract/ 

 

References

Kawagishi et. al. “Hericenones C, D and E, stimulators of nerve growth factor (NGF)-synthesis, from the mushroom Hericium erinaceum” Tetrahedron Letters (1991)

 

Ma et. al. “Hericenones and erinacines: stimulators of nerve growth factor (NGF) biosynthesis in Hericium erinaceus” Mycology (2010)

 

Hiwatashi et. al. “Yamabushitake mushroom (Hericium erinaceus) improved lipid metabolism in mice fed a high-fat diet” Bioscience, Biotechnology and Biochemistry (2010)

 

Ma et. al. “Cytotoxic aromatic compounds from Hericium erinaceum” Journal of Antibiotics (2010)

 

Khan et. al. “Hericium erinaceus: an edible mushroom with medicinal values” Journal of Complementary and Integrative Medicine (2013)

 

Friedman “Chemistry, Nutrition, and Health-Promoting Properties of Hericium erinaceus (Lion’s Mane) Mushroom Fruiting Bodies and Mycelia and Their Bioactive Compounds” Journal of Agricultural and Food Chemistry (2015)

 

Thongbai et. al. “Hericium erinaceus, an amazing medicinal mushroom” Mycological Progress (2015)

 

Kushairi et. al. “Lion’s Mane Mushroom, Hericium erinaceus (Bull.: Fr.) Pers. Suppresses H2O2-Induced Oxidative Damage and LPS-Induced Inflammation in HT22 Hippocampal Neurons and BV2 Microglia” Antioxidants (2019)

 

Yang et. al. “Rapid Identification of Chemical Constituents in Hericium erinaceus Based on LC-MS/MS Metabolomics” Journal of Food Quality (2021)

 

Turk et. al. “Effect of culture conditions on the content of hericene A, an α-glucosidase inhibitory constituent of Hericium erinaceus” Scientia Horticulturae (2021)

 

Martínez-Mármol et. al. “Hericerin derivatives activates a pan-neurotrophic pathway in central hippocampal neurons converging to ERK1/2 signaling enhancing spatial memory” Journal of Neurochemistry (2023)

 

Szućko-Kociuba et. al. “Neurotrophic and Neuroprotective Effects of Hericium erinaceus” International Journal of Molecular Science (2023)

 

Docherty et. al. “The Acute and Chronic Effects of Lion’s Mane Mushroom Supplementation on Cognitive Function, Stress and Mood in Young Adults: A Double-Blind, Parallel Groups, Pilot Study” Nutrients (2023)

 Cordyceps Mushrooms: Nature’s Enigmatic Fungi

 

Fig 1: Cordyceps militaris also known as the Scarlet Caterpillarclub Fungus (image)

What is Cordyceps?

Cordyceps mushrooms are an ancient group of entomopathogenic fungi (we’ll get to that in a minute) that have been around for millions of years. The oldest fossil evidence indicates that these fungi may have evolved more than 48 million years ago. Over 750 species of these awesome fungi have been discovered to date. In recent years the genus Cordyceps mushrooms was split into four: Cordyceps mushrooms, Ophiocordyceps, Metacordyceps, and Elaphocordyceps. Species from these genera have been used in traditional medicines for many years and share many behavioral characteristics.

Fig 2: Ophiocordyceps unilateralis also known as the Zombie Ant Fungus infects ants and controls their behaviour (image)

What is Entomopathogenic?

The term entomopathogenic refers to those organisms which can infect arthropods such as insects and caterpillars. This is one of the most unique aspects of fungi belonging to the Cordyceps genus. Most species of Cordyceps mushrooms require an insect host to complete their life cycle. In the absence of the host organism, the fungal population begins to decline.

Fig 3: Metarhizium robertsii is often used as a biocontrol agent against agricultural pests (image)

Anatomy of Cordyceps mushrooms

Similar to all fungi, Cordyceps mushrooms are composed of mycelium. Most of this mycelium grows vegetatively when it finds a useful nutrient source such as vegetative matter or an insect host. This insect body colonized by the Cordyceps mushrooms mycelia is also known as a sclerotium. The fruiting body of these fungi burst through the insect’s head or thorax and is called the stroma.

 The stroma is analogous to the stipe of many other fungi. The stroma reaches out of the substrate to bear perithecia (perithecium sing.) which are the spore-bearing organs of these fungi. Perithecia house millions of ascospores which are shed into the wind when the fungus is ready to propagate.

Fig 4: Cartoon representation of an Ophiocordyceps sinensis specimen highlighting the various anatomical features (image)

Life cycle of Cordyceps mushrooms

Like most fungi, Cordyceps mushrooms reproduce through spores. The spores are flung far and wide in search of favorable conditions for germination. Some species such as Cordyceps militaris can germinate and form hyphae in the soil and infect a passing insect larva later in its life, while others like Ophiocordyceps sinensis require an insect host to germinate and grow.

In either case, once the insect or larva has been infected, the fungus feeds on it and grows. It coaxes the insect host to climb up a high tree, branch, or rock and root itself in a windy area by biting down on the surface of the branch. The fungus then feeds on the insect and grows. At the end of its vegetative growth, it produces fruiting bodies or stroma which bear spores. The stroma pierces through the insect’s carapace and extends out into the atmosphere. The spores are then spread from the perithecia in the wind and the cycle continues.

This intricate dance between Cordyceps mushrooms and their host showcases the adaptability and resourcefulness of these fungi in the natural world. It has even inspired games such as ‘The Last of Us’. Rest assured that there is no threat of Cordyceps mushrooms suddenly evolving to infect humans and the game is purely a work of fiction. Or is it…

Fig 5: Lifecycle of Cordyceps fungi showing its parasitic nature (image) 

 

Geographic distribution

Cordyceps fungi are distributed across various ecosystems worldwide. While they are most commonly associated with tropical and temperate forests, different species have adapted to diverse environments, including alpine regions, grasslands, and even the frigid expanses of the Arctic.

Cordyceps militaris is one of the most widespread members of this family and can be found across Asia, Europe, and the Americas. It has a wide range of insect species that it can infect and is highly adaptable. However, for unknown reasons, their densities in the wild are relatively less and they are a rare find.

Fig 6: Geographic distribution of Ophiocordyceps sinensis (image)

Ophiocordyceps sinensis on the other hand is highly restricted in its distribution. It is primarily found in the Himalayan region in parts of Bhutan, Tibet, China, and India. They require an insect host (Ghost moth larvae) and can often be observed in the forests of these regions. Most of the O. sinensis consumption is from wild harvested fruiting bodies. 

Types of Cordyceps mushrooms

 

Fig 7: Ophiocordyceps sinensis (image)

Ophiocordyceps sinensis: The ‘OG’

Medicinal Significance: Ophiocordyceps sinensis, commonly known as the “Himalayan Viagra,” is a prized species in traditional Chinese medicine. It infects caterpillars in the Himalayan region and has been traditionally used for its purported aphrodisiac properties and the ability to enhance vitality. Research indicates potential immunomodulatory effects, making it a subject of interest for conditions related to immune system health. It is mostly wild harvested as the methods of cultivation still need significant improvement.

Fig 8: Cordyceps militaris 

Cordyceps militaris: The cultivable wonder

Cordyceps militaris is one of the few Cordyceps species that can be cultivated artificially. This has led to its increased popularity in commercial products, making it more accessible for various applications. Studies suggest that Cordyceps militaris contains bioactive compounds such as cordycepin, which has been associated with anti-fatigue and anti-inflammatory properties.

Fig 9: Ophiocordyceps unilateralis

Ophiocordyceps unilateralis: The “Zombie Ant” Fungus

Ophiocordyceps unilateralis, also known as the ‘Zombie Ant fungus’ is ruthless in its ability to manipulate the behavior of ants. Infected ants are compelled to climb vegetation and fiercely bite down onto the branch with their mandibles in what is called a ‘death grip’. The fungus then parasitizes the ant eventually producing a stroma that bursts out from the head of the ant. facilitating optimal spore dispersal. They are predominantly found in tropical forest ecosystems.

Fig 10: Cordyceps cicadae

Cordyceps cicadae: Harmonizing with Cicadas

Cordyceps cicadae as the name suggests, parasitise cicadas in their nymph stage. This species is prevalent in regions where cicadas are abundant. Studies have shown that these fungi have similar bioactive molecules to O. sinensis and are a potential alternative to O. sinensis cultivation. It has been used in folk medicine to treat malaria, palpitations, cancer, fever, diabetes, eye diseases, dizziness, and chronic kidney diseases.

Fig 11: Ophiocordyceps gracilis (image)

Ophiocordyceps gracilis: The Alpine Specialist

Ophiocordyceps gracilis is closely related to O. sinensis and has been widely used in traditional Chinese medicine. It has potent antioxidant properties. It is often sold in place of O. sinensis due to the similarity in their appearance.

Fig 12: Cordyceps robertsii 

Cordyceps robertsii: The tattoo artist

Cordyceps robertsii is native to New Zealand and displays a broad host range, infecting various caterpillars dwelling in leaf litter. These infected caterpillars have been used in traditional Maori tattoos as an ink. The caterpillars are burnt and the pigments are extracted using fat. The ink is said to possess antiseptic properties and prevent infections on the tattoo

Fig 13: Cordyceps subsessilis

Cordyceps subsessilis: The surgeon’s friend

Cordyceps subsessilis challenges the traditional association of Cordyceps mushrooms with forests by thriving in grasslands and meadows. In its vegetative form, it grows freely in the soil. However, when an unwitting scarab beetle walks by, it latches on and begins the sexual part of its life cycle. The vegetative form of this fungus is also named Tylopocladium inflatum and is the source of the life-saving immunosuppressant cyclosporine which has made organ transplants possible.

Cultivation: The art of growing Cordyceps mushrooms

In ancient times, O. sinensis was the primary species that was consumed mainly in parts of China. It has even been referred to as “Himalayan Viagra” due to its libido-enhancing properties. However, due to the difficulty in cultivating O. sinensis, its widespread use is restricted. 

The cultivation of C. militaris has been a breakthrough in Cordyceps mushrooms research and development as it has led to the possibility of this mushroom being consumed all across the world. The different ways in which Cordyceps mushrooms are cultivated are:

Fig 14: Cordyceps militaris can be cultivated at scale using wheat, oat, or rice as a substrate (image)

Substrate-Based Cultivation

In substrate-based cultivation, the focus is on mimicking the natural conditions that facilitate Cordyceps mushrooms’ growth. This method involves preparing a nutrient-rich substrate, often composed of grains such as rice or millet, which serves as the medium for fungal growth. The spores or mycelium of Cordyceps mushrooms are introduced into the substrate, and under controlled conditions of temperature and humidity, the fungus proliferates. This method is popular for its simplicity and ability to scale production.

Fig 15: Fermentation vessels much like those used for beer can be used to grow mycelia at large scales (image)

Liquid Fermentation

Liquid fermentation involves cultivating Cordyceps mushrooms in liquid nutrient mediums. This method is particularly useful for species that are challenging to cultivate on solid substrates. The liquid medium provides an environment conducive to the growth of mycelium, and it allows for better control over nutrient composition. Liquid fermentation has been employed for various Cordyceps species, offering flexibility and scalability in production.

Fig 16: Ophiocordyceps sinensis is often cultivated by manually injecting spores or mycelia into insect larvae (image)

Insect-Based Cultivation

The goal of this method is to mimic the natural parasitic relationship between Cordyceps mushrooms and insects. In this method, insect larvae are infected with Cordyceps mushroom spores. The infected insects are then placed in a controlled environment conducive to fungal growth similar to substrate-based cultivation. This method provides a more natural habitat for Cordyceps mushrooms and has been employed for specific species that exhibit a preference for insect hosts such as O. sinensis. 

Hybrid Cultivation Approaches

Hybrid cultivation approaches combine elements of substrate-based, liquid fermentation, and insect-based methods. These integrated systems aim to capitalize on the strengths of each approach, optimizing conditions for Cordyceps mushroom growth and enhancing overall yield.

While cultivation methods for Cordyceps mushrooms have advanced significantly, challenges persist. Maintaining the specific ecological conditions in which Cordyceps mushrooms thrive in the wild can be complex. Many strains of Cordyceps mushrooms start to degenerate after a few generations of cultivation leading to greatly reduced fruiting body yields and minimal bioactive molecule production.

The allure of wild-harvested Cordyceps mushrooms has been ingrained in cultural practices and traditional medicine and the increasing demand for these fungi will soon surpass the number of naturally available specimens. To truly unlock the health-promoting benefits of these organisms, new approaches and methods of cultivation need to be constantly developed.

Unveiling the Potent Compounds Within Cordyceps mushrooms

Cordyceps mushrooms are a treasure trove of bioactive molecules that have piqued the interest of researchers and healthcare professionals. These compounds, found in varying concentrations across different Cordyceps species, contribute to the fungi’s potential therapeutic properties:

Fig 17: Beta glucans are polysaccharides found in Cordyceps cell walls

Polysaccharides

Among the most studied bioactive molecules in Cordyceps mushrooms are polysaccharides. These complex carbohydrates play a crucial role in the fungi’s adaptogenic and immunomodulatory effects. Beta-glucans in particular have been linked to enhanced immune system function, antioxidative properties, and potential anti-inflammatory effects. 

Apart from beta-glucans, exopolysaccharides (EPS) isolated from Cordyceps mushrooms fermentation media have also been shown to possess anti-inflammatory properties.

Fig 18: Cordycepin and Adenosine (image)

Cordycepin

Cordycepin is a nucleoside analog and a prominent bioactive compound found in Cordyceps mushrooms. It is largely attributed as the source of the endurance-enhancing effects of Cordyceps mushrooms and their extracts. It can directly interact with energy-regulating systems within the cells and modulate cellular respiration. It also has a significant role to play in the respiratory health benefits of Cordyceps mushrooms.

Adenosine

Adenosine, a purine nucleoside, is found in Cordyceps mushrooms and is closely related to cordycepin. It plays a role in various physiological processes, including the regulation of inflammation and immune responses. It is an integral component of cellular signaling and DNA.

Fig 19: Ergosterol a precursor to Vitamin D2 (image)

Ergosterol

Ergosterol, a sterol compound found in Cordyceps mushrooms, is a precursor to vitamin D2. Beyond its role in vitamin D synthesis, ergosterol has been associated with potential antioxidant and anti-inflammatory effects. 

Fig 20: Cordycepic acid (image)

Cordycepic Acid (D-Mannitol)

Cordycepic acid, also known as D-mannitol, is a sugar alcohol found in Cordyceps mushrooms. It has been studied for its potential anti-inflammatory and antioxidant properties. It promotes liver and kidney health.

Cordymin

Cordymin, a peptide, has been found in water extracts of Cordyceps mushrooms. It has been shown to possess antifungal, anti-inflammatory, anti-nociceptive, and antiproliferative activities. It has also been shown to significantly improve the symptoms of diabetic osteopenia (weakening of bones during diabetes) in mice. 

Understanding the diverse array of bioactive molecules in Cordyceps mushrooms opens up avenues for targeted research and applications in medicine. As scientists delve deeper into the specifics of these compounds, the potential for harnessing Cordyceps mushrooms’ pharmacological properties becomes increasingly promising.

Fruiting Bodies vs. Powders vs. Extracts

Fig 21: Cordyceps militaris fruiting bodies are the purest form (image)

Cordyceps mushrooms can be consumed as whole fruiting bodies, powders, or extracts. The choice of consumption is purely up to the user. Fruiting bodies are the most similar to the native form of the mushroom and can be consumed dried or fresh. Most commonly they are used in broths or infusions like tea.

Fig 22: Cordyceps militaris powders are good substitutes for fruiting bodies (image)

Powders are very similar to whole fruiting bodies except they are ground into a fine powder. They can be consumed similarly to fruiting bodies, as stews or teas, and can also be found in capsules.

Extracts are the most effective method of consuming Cordyceps mushrooms as they contain the highest concentration of bioactive molecules. Unlike most other mushrooms, the majority of the known bioactive molecules from Cordyceps mushrooms are water-soluble. While dual extracts are not necessary to reap the benefits from these molecules, they may have an advantage over just water extracts since they increase the efficiency of extraction of the bioactive molecules. 

 

Fig 23: Extracts are a great way to consume concentrated bioactive molecules

Due to the relatively recent explorations into the bioactive molecules in Cordyceps mushrooms, there may be alcohol-soluble compounds yet to be discovered that have great beneficial effects so consumption of dual extracts is still recommended.

Cordyceps mushrooms for Health and Wellbeing

Cordyceps mushrooms have been used in traditional Chinese medicine for centuries. Modern research is identifying the mechanisms by which Cordyceps mushrooms exert their beneficial properties. Over 2,300 research papers are available on these fungi. A search highlighting the health benefits of Cordyceps mushrooms returned more than 350 papers with the number of papers published per year, steadily on the rise. 

Most of the existing studies are performed in animal models, the increasing number of clinical trials aims to find out if similar effects are seen in humans. Some of the key benefits of Cordyceps mushroom consumption are:

• Boost Physical Performance: Cordyceps mushrooms particularly cordycepin have been shown to increase the number of adenosine triphosphate (ATP) molecules within cells of the body. This effect allows athletes to sustain physical effort for longer periods
• Boost Mental Performance: Just like in physical performance, the boost in cellular energy has been shown to support mental effort for long periods and therefore Cordyceps mushrooms can also be used when working long hours
• Anti-Aging Effects: Cordyceps mushroom extracts have been shown to significantly increase the lifespan of mice and flies compared to placebo. These effects have been attributed to the antioxidant and metabolism-boosting properties of cordyceps extracts
• Anti Fatigue Effects: Cordyceps mushrooms have been shown to reduce fatigue and have been used to treat chronic fatigue. 
• Support Lung Health: Cordyceps mushroom extracts have traditionally been used to treat respiratory disorders. Modern studies have shown them to be effective in reducing the symptoms of chronic obstructive pulmonary disorder (COPD) and lung fibrosis.
• Boosting Sexual Performance: Cordyceps mushrooms have long been revered for their ability to boost libido and enhance sexual performance. This ability has earned it the nickname “Himalayan Viagra” in Eastern medicine. 
• Antidiabetic Effect: Cordyceps mushrooms have been used to treat diabetes in mouse models of the disease. It has been shown to decrease blood glucose levels and also protect the kidney from damage in diabetic mice.
• Support Heart Health: Cordyceps mushroom extracts have been effective in lowering cholesterol and triglyceride levels in animal models. This indirectly improves cardiovascular health and reduces the risks of heart disease.

Bridging Tradition and Evidence: Unraveling Cordyceps Mushrooms’ Impact on Human Health

The potential health benefits of Cordyceps mushrooms, rooted in traditional medicine, have sparked scientific curiosity, leading to a growing number of clinical trials. Over 20 clinical trials are being conducted for various indications using cordyceps mushrooms or their extracts. These trials aim to validate the therapeutic claims associated with Cordyceps mushrooms and provide a foundation for evidence-based recommendations. 

While a deep dive into the different clinical trials of Cordyceps mushrooms is outside the scope of this blog, both cordyceps fruiting body powders and cordyceps extracts are being tested for their performance-boosting, anti-cancer, antidiabetic, and immune supportive properties among others.

Echoes of Centuries: Cordyceps mushrooms in Traditional Healing Practices

Long before the advent of modern clinical trials and scientific investigations, Cordyceps mushrooms had already found a revered place in traditional healing practices across various cultures. This section delves into the rich tapestry of traditional uses, folklore, and cultural significance surrounding Cordyceps mushrooms, providing a historical context to its contemporary exploration.

Traditional Chinese Medicine (TCM)

In Traditional Chinese Medicine (TCM), Cordyceps mushrooms have been esteemed for centuries as a potent tonic with adaptogenic properties. It is believed to nourish the lungs and kidneys, fortify the immune system, and promote longevity, and is often associated with enhancing vitality.

Himalayan Folk Medicine

In the Himalayan region, Cordyceps mushrooms hold a special place in the folklore and traditional medicine of communities. Ophiocordyceps sinensis, known locally as “Yarsagumba” or the “Himalayan Viagra,” is believed to have aphrodisiac properties and is used to address a range of health concerns.

Tibetan Medicine

Cordyceps mushrooms have also found a significant role in Tibetan medicine, where it is believed to balance the body’s elements and energies. It is traditionally used for conditions related to the lungs, kidneys, and reproductive health. The holistic approach of Tibetan medicine integrates Cordyceps mushrooms into formulations aimed at restoring harmony within the body.

Conclusion

The traditional use of Cordyceps mushrooms is not merely about the physical application of a natural substance but reflects a profound cultural and generational wisdom. Knowledge about the timing of harvest, preparation methods, and specific use-cases has been passed down through generations, creating a legacy that continues to shape the relationship between communities and Cordyceps mushrooms.

As modern scientific methods are applied to the traditional systems of healing, we are beginning to discover the diversity of molecules that allow Cordyceps mushrooms to have their healing properties. Better methods of cultivation and extraction are aimed at maximizing these health benefits and improving the quality of supplements available in the market. 

Cordyceps mushrooms have the power to enhance one’s pursuit of physical fitness and cellular metabolic health. These are the bedrock of a long and productive life. At Nuvedo, we have been awestruck by the majesty of these unique mushrooms. Our ultrasound-based extraction technology was designed to maximize the amount of bioactive compounds. 

Interested in incorporating Cordyceps extracts into your routine? Check out our selection of high-quality Cordyceps extract: https://nuvedo.com/product/cordyceps-liquid-extract/

References

Zhang et. al. “Ophiocordyceps sinensis, the flagship fungus of China: terminology, life strategy and ecology”. Mycology (2012)

Srestha et. al. “The medicinal fungus Cordyceps militaris: research and development”. Mycological Progress (2012)

Ashraf et. al. “Cordycepin for Health and Wellbeing: A Potent Bioactive Metabolite of an Entomopathogenic Medicinal Fungus Cordyceps with Its Nutraceutical and Therapeutic Potential”. Molecules (2020)

Liu et. al. “The Chemical Constituents and Pharmacological Actions of Cordyceps sinensis”. Evidence-Based Complementary and Alternative Medicine (2015)

Wong et. al. “Cordymin, an antifungal peptide from the medicinal fungus Cordyceps militaris” Phytomedicine (2011)

Qi et. al. “The Protective Effect of Cordymin, a Peptide Purified from the Medicinal Mushroom Cordyceps sinensis, on Diabetic Osteopenia in Alloxan-Induced Diabetic Rats” Evidence-Based Complementary and Alternative Medicine (2013)

Qian et. al. “Anti-inflammatory and antinociceptive effects of cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis” Natural Product research (2012)

Tan et. al. “The lifespan-extending effect of Cordyceps sinensis Cs-4 in normal mice and its molecular mechanisms” FASEB (2011)

Zou et. al. “Cordyceps sinensis oral liquid prolongs the lifespan of the fruit fly, Drosophila melanogaster, by inhibiting oxidative stress” International Journal of Molecular Medicine (2015)

Chai et. al. “Cordycepin exhibits anti-fatigue effect via activating TIGAR/SIRT1/PGC-1α signaling pathway” Biochemical and Biophysical Research Communications (2022)

Song et. al. “Studies on the Antifatigue Activities of Cordyceps militaris Fruit Body Extract in Mouse Model” Hindawi (2015)

Yu et. al. “Hypoglycemic Activity through a Novel Combination of Fruiting Body and Mycelia of Cordyceps militaris in High-Fat Diet-Induced Type 2 Diabetes Mellitus Mice” Journal of Diabetes Research (2015)

Liu et. al. “Antidiabetic and Antinephritic Activities of Aqueous Extract of Cordyceps militaris Fruit Body in Diet-Streptozotocin-Induced Diabetic Sprague Dawley Rats” Oxidative Medicine and Cellular Longevity (2016)

Guo et. al. “Cordycepin prevents hyperlipidemia in hamsters fed a high-fat diet via activation of AMP-activated protein kinase” Journal of Pharmacological Sciences (2010)

Yang et, al. “Cordyceps sinensis inhibits airway remodeling in rats with chronic obstructive pulmonary disease” Experimental and Therapeutic Medicine (2018)

Chen et. al. “Protective roles of Cordyceps on lung fibrosis in cellular and rat models” Journal of Ethnopharmacology (2012) 

What is Reishi?

Fig 1: Reishi mushroom (Ganoderma lucidum) can be identified by its shiny appearance and woody texture

 

Reishi mushroom is revered in traditional Chinese medicine as the “Herb of immortality” or “king of herbs”. Its scientific name is Ganoderma lucidum. Over the centuries it has been used to symbolize wealth and longevity. Its many medicinal properties have made it the subject of both ancient as well as modern scientific inquiry. With over 3800 publications and 40 clinical trials, Reishi mushrooms hold the title of the most well-researched functional mushroom on Earth today.

Fig 2: The number of scientific studies containing the keyword Ganoderma has constantly increased

 

Where are Reishi mushrooms found?

Reishi mushrooms can be easily identified by their shiny lacquered appearance which is also the reason behind their Latin name Ganoderma which means “shiny skin”. They tend to be reddish brown and fan out in the shape of an ovoid bookshelf. They have a woody and fibrous texture which makes them difficult to consume directly.

They can be found growing all over the world but are particularly common in Asia. Their preferred substrate is hardwood trees which are dead or decaying, especially maple, oak, or elm.

Fig 3: Mushrooms belonging to the Ganoderma genus can be found all over the world (Source)

 

Are Reishi mushrooms safe to consume?

Consumption of Reishi mushroom can be traced back to the Han dynasty which ruled over China between 206 BC to 220 CE. Its usage was traditionally reserved for emperors and royalty. It was equally respected in Japanese culture where it was reserved for the imperial family and nobility. The regular consumption of Reishi by these Nobels laid the foundations for its global recognition today. In short, these mushrooms have been consumed safely for thousands of years without any documented ill effects.

The medicinal properties of Reishi were recorded in a classical text from Chinese traditional medicine, the Shennong’s herbal classic (Shennong Ben Cao Jing) from the Han dynasty. The text referred to the main effect of reishi as strengthening and restoring health.

Fig 4: Shennong Ben Cao Jing, an ancient Chinese text containing information on herbs used in traditional Chinese medicine (Source)

More recent evidence traced the usage of Reishi mushrooms to 6000 years old. Archeological sites from Zhejiang province in China excavated spores that were approximately 6817 years old making them some of the oldest evidence for Reishi mushroom usage by humans. 

Today reishi mushroom is the most popular medicinal mushroom consumed globally. Both powder and extracted forms are safe to consume if processed responsibly. In fact, there are large-scale clinical trials being conducted to investigate the effects of Reishi mushroom on humans.

As recently as 2015, Ganoderma extracts were included in the Chinese pharmacopeia, a list of all accepted medicinal compounds in use today in China. This acceptance is spreading throughout the world and Reishi mushrooms are fast becoming the most widely consumed medicinal mushrooms globally. 

What are the beneficial molecules in Reishi mushrooms?

 In the 20th century, there has been a surge in scientific interest in Reishi mushrooms. Many research studies have investigated the compounds in Reishi and their potential health benefits. These studies have aimed at validating some of the claims made by traditional medicine while discovering new uses.

Some of the key bioactive molecules found in Reishi mushrooms are:

 

Fig 5: Structure of a beta-glucan molecule the major immunomodulatory polysaccharide in Reishi mushrooms

 

Polysaccharides: Reishi mushrooms are rich in various polysaccharides, such as beta-glucans and heteropolysaccharides. Beta glucans in particular have immunomodulatory properties. They interact with immune cells to keep them in a state of alertness.

 

Fig 6: Ganoderic Acid A, a triterpene from Reishi mushroom

 

Triterpenes: Triterpenes are a group of compounds found in Reishi mushrooms, including ganoderic acids. These compounds are believed to have anti-inflammatory, antioxidant, and potential anti-cancer properties. They may also support liver health.

 

Peptides: Peptides are short sequences of amino acids attached to each other. Reishi mushrooms contain various bioactive peptides, some of which may have antioxidant and neuroprotective effects.

Fig 7: Structure of ergosterol, a steroid-like molecule found in Reishi mushrooms

 

Sterols: Sterols, such as ergosterol and ganoderol, are found in Reishi mushrooms. These compounds have potential cholesterol-lowering properties and may contribute to overall cardiovascular health. contribute to the production of Vitamin D.

Fig 8: Basic structure of Stilbene, a kind of polyphenol found in Reishi mushrooms

 

Polyphenols: Reishi mushrooms (Ganoderma lucidum) also contain polyphenols, which are a group of naturally occurring compounds with antioxidant properties. Polyphenols are known for their ability to neutralize harmful free radicals, reduce inflammation, and support various physiological processes in the body.

 

What is Reishi mushroom extract good for?

 

Reishi mushrooms have the widest-ranging documented health benefits of all medicinal mushrooms consumed to date. Some of the key health benefits are:

 

Immune system support: Beta glucans from Reishi mushrooms enhance the body’s ability to fight off infection and disease by boosting the immune system. They interact with immune cells and impart a state of alertness.

 

Anti-inflammatory effects: Both polysaccharides and triterpenes from Reishi mushrooms have anti-inflammatory properties. Reducing inflammation in the body has been proven to be beneficial to health over long periods.

 

Antioxidant properties: The antioxidant properties of Reishi mushrooms come primarily from polyphenols. They help combat oxidative stress and protect cells from damage caused by free radicals. This activity contributes to better cellular health and longevity.

 

Stress reduction: Some people use Reishi mushrooms to promote relaxation, reduce stress, and improve sleep quality. Compounds like adenosine found in Reishi are thought to play a role in these effects. The adaptogenic properties of Reishi help in managing stress and improving sleep.

 

Potential cancer-fighting properties: Bioactive molecules from Reishi mushroom are effective in fighting against cancer in lab studies on mice and cells. Beta-glucans support cells of the immune system that seek and destroy cancer cells, while terpenes and polyphenols can directly attack cancer cells.

 

Liver support: Water extracts of Reishi mushrooms have been shown to protect the liver against toxin-induced damage. Alcoholic extracts have also been shown to be effective in promoting liver metabolism and protecting from fat accumulation in the liver. Antioxidants such as polyphenols protect liver cells from oxidative stress and damage caused by free radicals.

 

Respiratory support: Due to the anti-inflammatory and immunomodulatory properties of Reishi mushrooms, they have been shown to reduce airway inflammation in mouse models of asthma. They also interfere in the molecular processes of lung cancer cells and cause them to die.

 

When should I take Reishi mushroom extract?

 

Due to their calming effects on the nervous system, it is recommended to consume Reishi mushroom extracts at a time when you’d like to wind down. Consuming Reishi mushroom extracts a couple of hours before sleep or deep rest has been shown to promote sleep quality.

 

Types of Reishi Mushroom Supplements

 

 

 

Fig 9: Reishi mushroom powder (source)

 

Powder: Powders are generally made from ground-up mushroom fruiting bodies. They contain the whole mushroom and all the molecules present in the mushroom fruiting bodies. However, most of the bioactive molecules are trapped within the walls of the mycelia and are inaccessible to our digestive systems.

 

Fig 10: Reishi mushroom being extracted by boiling in a vat with water (source)

 

Hot water extract: Hot water extracts are prepared by boiling whole or powdered mushrooms over long periods. The extracts prepared by this method are rich in polysaccharides. These extracts lack terpenes and polyphenols which are more soluble in water. Furthermore, the heat-sensitive molecules also degrade due to the long periods of heating.

 

Fig 11: Reishi mushroom tincture prepared by steeping cut fruiting bodies in alcohol (source)

 

Tincture: Tinctures are prepared by soaking whole mushrooms or mushroom powders in liquids with high alcohol content such as grain alcohol. The mushrooms are steeped in the alcohol for many weeks to extract hydrophobic molecules. Tinctures are rich in polyphenols and terpenes but contain no polysaccharides.

 

Dual Extract: Dual extracts are prepared by combining hot water extracts and tinctures. They contain the widest range of bioactive molecules. They are the most beneficial to consume when targeting overall health benefits.

 

Ultrasound-Assisted Extracts: Ultrasound-assisted extracts are a new method for extracting bioactive molecules from mushrooms. In this method, high-frequency sound waves are used to break open the cells of the mycelia to release bioactive molecules from the cells. They can be performed using water or ethanol. The process is much faster than the previously mentioned methods and contains more bioactive compounds than the other methods.

 

At Nuvedo we use ultrasound-assisted extraction to prepare our Reishi mushroom dual extracts. This allows us to produce the most potent extracts in the shortest period of time so we can bring you the widest range of health benefits in record time.

 

What is the best way to consume Reishi mushrooms?

 

Both extracts and powders have their advantages, and the choice between them can be influenced by factors such as concentration, convenience, and intended use. 

 

Preparation: Powders are prepared by finely grinding whole mushrooms or their spores. They are as good as the whole mushroom. Extracts are prepared by concentrating active compounds isolated from the mushrooms using solvents.

 

Taste: Mushroom powders taste similar to the whole mushroom. Extracts on the other hand can taste quite different from the whole mushroom as only some of the molecules present in the whole mushroom are available in the extract. 

 

Bioactive molecules: Mushroom powders contain the same bioactive molecules as a whole mushroom. Extracts contain specific bioactive molecules such as beta-glucans, polyphenols, and terpenes.

 

Potency: Mushroom powders have a low potency as the bioactive molecules are present in their natural concentrations. Mushroom extracts are significantly more potent and contain much higher concentrations of bioactive molecules compared to powders. One would have to consume much larger quantities of powders to get the same benefits.

 

Bioavailability: the bioactive molecules in mushroom powders are trapped within the tough cell walls of reishi mushroom mycelia. This makes them inaccessible to the digestive system. Extracts have high concentrations of freely available bioactive molecules. These molecules are more easily absorbed by the body.

 

Consumption: Mushroom powders can be consumed similarly to whole mushrooms by using them in soups, stews, or baked goods. Extracts are specially designed to provide functional benefits and are available as powders, concentrates, or capsules.

 

In our experience, extracts have been reported to have more reliable results and the dosages can be more precisely controlled. Nuvedo Reishi mushroom extracts are made using our proprietary ultrasound-assisted extraction process using both alcohol and water to maximize the bioactive molecule yield. We also concentrate our extracts so you can get the most bang for your buck.

Fig 12: Reishi mushroom extract being concentrated during our RnD process

Choosing the best Reishi mushroom products?

Due to the diverse nature of the molecules in Reishi mushrooms, it is always best to extract them with both water and alcohol. Dual extracts contain the widest range of bioactive molecules and are the most beneficial way to consume Reishi mushrooms.

We always recommend looking for extracts that have a certificate of analysis indicating the concentrations of bioactive molecules such as beta-glucans, polyphenols, and terpenes. Apart from this, we also recommend looking at test reports for heavy metal and microbial contamination to ensure that you’re getting the best quality product.

At Nuvedo, we are committed to producing the highest quality extracts by adopting the latest technologies. We strongly believe in your right as a consumer to make an informed decision. That is why we make the certificates of analysis for our extracts publicly available so you know exactly what you are getting when you buy our products.

 

Fig 13: Reishi mushrooms as an ingredient for daily health

 

Integrating Reishi Mushroom Extracts into Your Daily Routine

Incorporating Reishi mushroom extracts into your daily wellness routine can be a transformative step towards better health. Nuvedo’s Reishi extracts are designed for ease of use and can be integrated into various diets and lifestyles. Whether added to your morning smoothie, or evening tea or taken as a standalone supplement, these extracts can seamlessly become a part of your health regimen.

 

How much Reishi mushroom extract should I consume?

The appropriate dosage of Reishi mushroom extract can vary based on the product’s concentration and the individual’s health goals and conditions. Nuvedo recommends starting with a small dose to assess tolerance and gradually increasing as needed. It’s always advisable to consult with a healthcare professional, especially for those with pre-existing health conditions or who are taking other medications.

 

Fig 14: Nuvedo’s Reishi mushroom dual extract comes with a standardized dropper for consistent dosing

 

 What are the side effects of mushroom extract?

Unlike Reishi mushroom powders, there have been no documented side effects from the consumption of reishi mushroom extracts. In humans, high doses of reishi mushroom extracts, up to 10g/kg have been reported to be completely safe. 

 

How does Reishi mushroom make you feel?

As a result of the calming effects of Reishi mushroom extracts on the nervous system, they have a relaxing effect. Many people have reported feeling calm or sleepy after consuming Reishi mushroom extracts. These extracts are also known to promote good sleep which is beneficial for maintaining energy levels throughout the day.

 

Fig 15: Nuvedo’s Reishi mushroom infusion coupled with Ashwagandha and Lavender provides a relaxing end to a hectic day

 

Other uses of Reishi mushroom

While Reishi mushrooms are medicinal powerhouses, that’s not all they are good for. Recent years have seen a surge in investigations using mycelium as a material. Materials created using fungal mycelia are known as metamaterials.

Reishi mushroom is used as a preferred species for mycobacterials due to the toughness of their mycelium and their highly adaptive nature. They can be easily grown on a wide range of substrates. Substrates are generally plant-derived materials such as sawdust.

Using these methods, people have produced a wide range of materials such as insulation panels, leather substitutes, and even furniture. We too have used our Reishi mushroom cultures to generate pieces of art like no other. We unite biomaterials with the boundless creativity of artists, fostering an environment that nurtures innovation and fosters the seamless amalgamation of art and science.

 

Fig 16: The Mycobloc chair, a collaborative art project between Nuvedo and artist Maria Joseph

 

The Mycobloc Chair was an experiment in sustainability, combining materials from two ages into one mind-bending art piece. The juxtaposition of plastic, a material of the previous technological age that has immense short-term value but in the long-term is a pollutant that contaminates ecosystems everywhere, and mycelium. Mycelium is the biomaterial of the future. Mycelium-based materials have many desirable properties that make them a prime replacement for plastics. 

Mycelium-based materials have been proven to be excellent insulators and are the frontrunners to be the next best replacement for styrofoam. They are also flame and chemical retardant which makes them excellent replacements for foams and panels used for home insulation. The greatest advantage of mycomaterials is their completely biodegradable nature. They can be readily composted after their useful lifetime, thus returning the nutrients to the earth from where they came.

While our primary focus at Nuvedo centers on crafting functional mushroom extracts and products, our commitment extends beyond this realm. We engage in projects like these to shed light on the ecological impact of fungi, a purpose that seamlessly weaves into our ethos and practices. It is our profound understanding of the broader significance of mycelium and its role in shaping our future that propels us forward.

 

Interested in incorporating Reishi extract into your routine? Check out our selection of high-quality Reishi extract: https://nuvedo.com/product/reishi-liquid-extract/

References

Wu et. al. “Hepatoprotective effects of aqueous extract from Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (higher basidiomycetes) on α-amanitin-induced liver injury in mice”. (2013) DOI: 10.1615/intjmedmushr.v15.i4.60

Ahmad et. al. “Ganoderma lucidum: Novel Insight into Hepatoprotective Potential with Mechanisms of Action”. (2023) DOI: 10.3390/nu15081874

Li et. al. “Ganoderma modulates allergic asthma pathologic features via anti-inflammatory effects”. (2022) DOI:10.1016/j.resp.2022.103843

Figlas and Curvetto. “Medicinal mushroom Reishi (Ganoderma Lucidum) – Toxicity and allergenicity studies. Dosage, posology and side effects” 

Yao et. al. “Ganoderma lucidum promotes sleep through a gut microbiota-dependent and . serotonin-involved pathway in mice” (2021) DOI: 10.1038/s41598-021-92913-6

Sharma et. al. “Bioactive metabolites of Ganoderma lucidum: Factors, mechanism and broad spectrum therapeutic potential”. (2019) DOI: 10.1016/j.hermed.2019.100268

Wu et. al. “Anti-cancer properties of triterpenoids isolated from Ganoderma lucidum – a review”. (2013) DOI: 10.1517/13543784.2013.805202

Wu et. al. “Triterpenoids from Ganoderma lucidum and Their Potential Anti-inflammatory Effects”. (2019) DOI: 10.1021/acs.jafc.9b01195

Veljović et. al. “Chemical composition, antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts”. (2017) DOI: 10.1007/s13197-017-2559-y

Cen et. al. “Anti-tumor and Anti-angiogenic Ergosterols from Ganoderma lucidum”. (2017) DOI: 10.3389/fchem.2017.00085

Xu et. al. “Anti-inflammatory effects of Ganoderma lucidum sterols via attenuation of the p38 MAPK and NF-κB pathways in LPS-induced RAW 264.7 macrophages”. (2021) DOI: 10.1016/j.fct.2021.112073

Wu et. al. “Sterols and triterpenoids from Ganoderma lucidum and their reversal activities of tumor multidrug resistance”. (2020) DOI: 10.1080/14786419.2021.1878514

Seweryn et. al. “Health-Promoting of Polysaccharides Extracted from Ganoderma lucidum”. (2021) DOI: 10.3390/nu13082725

Wang et. al. “Traditional uses, chemical components and pharmacological activities of the genus Ganoderma P. Karst.: a review”. (2020) DOI: 10.1039/D0RA07219B

Introduction

Nestled within the embrace of the majestic Western Ghats, Kerala’s landscapes boast an awe-inspiring fungal diversity, a testament to nature’s ingenuity. From vibrant orchids to elusive fungi, this biodiversity is a global marvel that resonates with our national pride.

This symphony of life is an integral part of India’s rich heritage. Yet, the encroachment of human activity and the changing climate threaten this legacy. The urgency now compels us to stand united as custodians, preserving this irreplaceable gift for our nation and the world. Kerala, a southwestern state of India, is adorned with an intricate tapestry of ancient tribal cultures that have flourished for centuries. These tribes, such as the Irulas, Kurichiyas, and Paniyas, hold the threads of a rich cultural heritage, contributing to the diverse fabric of the region. The annals of history carry records of their existence, revealing a timeless connection to the land and its resources. Tribal communities are widespread across Kerala’s districts, with a significant concentration in Wayanad, Palakkad, Idukki, and Kasargod. These areas harbor the majority of tribes, reflecting their rich cultural diversity. However, Alappuzha stands out with the lowest tribal population among the districts.

Have you ever wondered how Kerala’s ancient tribes discovered the hidden secrets of plants and fungi long before the advent of modern science? This blog is a small peek into the world that they have uncovered.

Imagine this scene: tribes like the Irulas and Kurichiyas crafting their own versions of “green medicine cabinets” centuries ago. How did they know which plants could soothe a headache or which fungi could be the cure for the common cold? It’s like they were the original pharmacists, operating without a prescription pad!

And let’s not forget ethnomycology – the art of understanding fungi. While we’re still trying to pronounce the Latin names of mushrooms, these tribes have been incorporating Malayalam and its dialects for many species of fungi for ages. Did they possess a mystical dance that could unveil hidden mushrooms? Or perhaps, did they engage in a mycology-infused quest? akin to a treasure hunt straight from the heart of our tribal heritage?

Could their harmonious ways teach us a thing or two about sustainable living and preserving biodiversity? Maybe it’s time we took a leaf – or a mushroom – out of their book and rekindled our curiosity about the wild world around us. Who knows, we might even discover that our next breakthrough could be hidden in the forest, right beneath our curious noses!

 

 

Tribal Communities of Kerala

Imagine a mushroom buffet straight from the whimsical corners of Kerala! Hold onto your taste buds – there are around 40 different edible mushrooms in town, and about half a dozen of these are like the rockstars of the mushroom world. But here’s the twist: These fungal delicacies aren’t available year-round. They’re like the party guests who only show up after the monsoons hit – both the wild dancers, Edavapathi (south-west monsoon), and the cool breezes, Thulavarsham (north-east monsoon).

Now, let’s play hide and seek with these mushrooms in their favorite hideouts. They’re like little seasonal adventurers – popping up in plantations, hanging out at the edge of forests, strutting their stuff along forest paths, and even throwing shade inside bamboo breaks. Oh, and don’t be surprised if you spot them having a field day in fallow fields, on termite mounds, or chilling by the riversides.

And speaking of trees, some mushrooms are real tree huggers – you might spot them on tree trunks and snacking on decayed woods in the woods (yes, that’s a mouthful!). The real headliners of this shroom show are Arikkoon, Puttukoon, and Perumkali. These guys aren’t just delicious, they’re like habitat snobs too. Arikkoon and Puttukoon are the life of the termite mound party, while Perumkali prefers to strut its stuff in the open, moist areas, where the ghosts of old termite mounds still hang around.

 

So, next time you’re in Kerala, keep an eye out for these fungi fiestas. Who knows, you might just stumble upon a hidden mushroom rave in the middle of the forest!

From a paper titled,“Nutritional and biochemical studies of wild edible mushrooms used by tribes of Palghat and Wayanad districts of Kerala” – A fascinating journey into Attappadi’s tribal communities has uncovered a remarkable revelation – the taxonomic identification of wild edible mushrooms. An early documentation from 2018, of the tribal knowledge from the area. There were a total of 19 distinct edible delicacy species had been unveiled, spanning 12 genera, 9 families, and 4 orders. Astonishingly, 17 of these species are entirely new additions to the wild edible food repertoire of the Wayanad tribes, adding a new chapter to their culinary heritage.

 

 

Varieties like Auricularia delicata, Lentinus cladopus, Favolus tenuiculus, Schizophyllum commune, and Favolaschia manipularis, previously absent from records, now grace our tables. This tale unfolds as a journey into Kerala’s edible mushroom secrets, unearthing both gastronomic and medicinal promise.

Amidst this bounty, Lentinus bambusinus, Coprinellus micaceus, and Cantharellus minor emerged as first-time subjects for nutritional study.
Their flavors and textures piqued curiosity, hinting at untapped livelihood prospects and even groundbreaking drug discoveries.

Paniyas: The Largest Tribal Community in Kerala

 

The Paniyas stands as the largest tribal community in Kerala, adding their vibrant threads to the state’s cultural fabric. In their forays into the forests, they’ve discovered an array of mushrooms and fungi that become an integral part of their culinary heritage.

United by their reverence for Kumman—mushrooms. They sing praises to Marakkumman, the tree-dwelling mushrooms as if the forest itself orchestrates a delicate symphony. Yet, it is Mannukkumman, the earthbound fungi, that binds their roots to the soil of their ancestors.

Each variety bears a name that echoes through generations – Valakkumma, sprouting from cow dung and compost, symbolizes the cycle of life and renewal; Vaikkolkkumma, rising from paddy straw, mirrors the dance of harvest and gratitude.

Kathukkumman, akin to human earlobes, is their treasured gem. With the monsoon’s touch, it adorns Murikku trees—a sight reserved for the Paniya. This delicate wonder transforms into sustenance, a sacred thread connecting their past and present.

In this dance with nature, the Paniya commune with whispers of the forest, finding solace in the smallest offerings of the earth. Their heritage blooms like Kathukkumman, reminding us of nature’s power to unite generations and kindred spirits across cultures.

Paniya women, in particular, possess a remarkable memory of seasonal mushroom availability, linked to precise times and distinct locales. This practice underscores their profound connection with nature. However, the current scenario necessitates arduous journeys to remote, less polluted areas for these mushrooms. This dual struggle epitomizes the resilience of feminism and tribal heritage—a testament to their enduring significance. Often seen as the protectors of termite mounds, and finding unique ways to preserve mushrooms.

 

Kurumbas: The Guardians of the Forest

Meet the Kurumbas, the guardians of the forest in Kerala. These tribal custodians boast an unparalleled connection with the woodlands, fostering a harmony between their way of life and nature’s rhythms. Amidst their daily routines, they come across mushrooms that weave seamlessly into their sustenance practices. Perhaps they’re well-versed in distinguishing the edible from the inedible, cherishing the flavorful goodness of Puttukoon and other forest finds.

Apart from mushrooms, this intriguing community collects tubers and potherbs. They communicate through a blend of Malayalam-Tamil, while women attire themselves in munda cloth and palm leaf-rolled earrings. Rooted in animism, they now revere Bagawati, a genderless deity, and are guided by the Nolumbukaran priests.

Kuruma culture embraces the term ‘Koonu’ from Malayalam to celebrate mushrooms, a prideful inclusion in their family meals. This term references around 14 mushroom species, notably favoring those on soil and termite mounds. Perumkoonu, Arikoonu, Nedumthali, and Puttukoonu (Termitomyces sp.) are cherished varieties. Unlike other cultures, Kuruma women uniquely undertake mushroom preservation by proficiently drying them. These dried mushrooms retain quality until the next season, a technique borne of their culinary ingenuity.

Interestingly, the mushroom collection is exclusively designated for Kuruma women, involving the gathering of safer types like Arikkoon and Puttukoonu, which necessitate minimal processing. Notably, mushrooms earmarked for processing and storage possess reduced mucilaginous substances and softer fiber coatings. These culinary experts immerse mushrooms in turmeric water for a day before smoke-drying them above the hearth.

Intriguingly, how do Kuruma women determine the suitability of mushrooms for extended storage? What spurred the development of their unique preservation practices amidst evolving culinary trends? These captivating questions uncover the intricate layers of Kuruma’s mushroom-related traditions, showcasing their profound connection with nature and the enduring role of women in safeguarding cultural culinary legacies.

 

Kattunaickans: The Honey Gatherers

Picture the Kattunaickans, deft honey gatherers who traverse the wilderness in search of golden sweetness. In their pursuit, they stumble upon mushrooms like Arikkoon and Perumkali, sprouting forth from the very ecosystems they roam. One of sustenance, connection, and a trove of mushrooms, known as ‘Anavae’ in their dialect. These 33 fungal species, each with a scientific name and a habitat tale, hold not just nourishment, but an invitation to ponder our place in the natural world.

Divided into three categories – the ‘Maranavae’ found on tree barks, ‘Huthaanavae’ on termite mounds, and ‘Mannanavae’ gracing the forest floor – these mushrooms paint a vivid portrait of diversity and adaptation. Enter the scientific realm, where ‘Huthaanavae’ belongs to the Termitomyces genus, attesting to the profound relationship between the fungi and the termites’ industrious homes.

The ‘Maranavae’ mushrooms, named after their arboreal companions, beckon us to the enchanting dance of symbiosis. Mushroom names are derived from their host trees—’Njeralanavae’ from Syzygium cumini, ‘Jalanavae’ from Dalbergia latifolia, and ‘Kavalanavae’ from Erythrina indica. This practice bridges nature and culture, prompting us to ponder our own heritage and relationship with our surroundings.

Yet, as we marvel at this harmonious existence, a stirring arises within. Do we, ensnared in the modern world’s frenzied pace, miss the essence of life’s communion? The Kattunaikkas’ reverence for these forest treasures provokes introspection. Are we forfeiting nature’s bounty for momentary conveniences?

Diving into the intriguing world of mushrooms, the community’s fascination lies with the Termitomyces species. Among these, the enigmatic Vellanavae boasts a milky white hue, the Ummanavae a delicate pale white, and the impressive Huthanavae, a large off-white variety. What’s captivating is their connection to termite mounts. Remarkably, these mushrooms are deemed safe, negating the need for elaborate preparation prior to consumption. Even youngsters fearlessly indulge in raw samples. Ummanavae and Vellanavae thrive in dense clusters, in contrast to the solitary or scattered growth of the Huthanavae. Intriguingly, the Mannanavae sprout from the soil, categorized by their solitary ventures or group endeavors. Within this community, a diverse assortment of about 15 such mushroom types finds its way to their plates, each offering a unique growth pattern. The names themselves evoke curiosity, inviting us to explore the mysteries concealed within.

Among the Kattunaikka community, the mastery of distinguishing toxic from edible mushrooms rests primarily with women, relying on cues like odor and color. How do they cultivate this skill and pass it down through generations? Remarkably, this ability isn’t limited to proximity; they can detect these fungi from a distance, showcasing an intimate knowledge of their surroundings. But who else in the community participates in this intricate tradition?

It’s fascinating that the practice involves not only women but also other members of the community. How does this collective engagement reinforce the cultural significance of mushroom collection? When harvesting mushrooms from tree trunks, the emphasis on selective harvesting to ensure regrowth is admirable. How is this wisdom imparted to the young ones, especially the enthusiastic Kattunaikka children?

Furthermore, the allure of some mushroom varieties being palatable even when consumed raw adds a unique dimension. How does this tie into the community’s relationship with nature and its culinary heritage? The multifaceted involvement and knowledge sharing within the Kattunaikka community exemplify a harmonious blend of tradition and practicality.

In the heart of this blog, stand 2 tribes, (Muthuvans and Kaani) nestled at the crossroads of border states. There may be a lot more that I am missing, but Like the enigmatic mushrooms that transcend conventional categories, these tribes straddle both Kerala and Tamil Nadu, embodying a narrative that persistently challenges the significance of the lines we draw on maps. Their roots run deeper than the ink of borders, existing long before we etched the concept of land ownership. They are the true inhabitants of the woods, guardians of ancient secrets whispered by trees.

To overlook their existence is to miss a vital piece of this intricate puzzle that weaves humanity and nature. What are these borders? Who are we without them?  These tribes remind us that our connection with the land precedes borders, awakening a yearning to explore the bonds we share with the wild and the wisdom they offer.

Muthuvans: The Story Weavers

The Muthuvans, skilled storytellers of their tribe, possess a unique ability to weave narratives that entwine the rich landscapes of Kerala. As they explore the wilderness, their tales might incorporate the mushrooms and fungi they encounter. The likes of Arikkoon and Perumkali become not only sustenance but also inspiration, embedding themselves in the stories that form the backbone of their cultural tapestry.

Kaani Tribe: The Culinary Kings

 

Ethnomycology in India: A Deep Dive

Across the globe, tribal people have recognized the value of these forest gems as dietary staples and potent healers. In Kerala, ‘Adivasis’ reside amidst the lush landscapes of the Western Ghats, their lives intertwined with the ancient rhythm of the forests. Cholanaikkans, Kurumbas, Kattunaikkans, Kadars, and Koragas, these primitive tribal groups, embody a cultural heritage as diverse as the mushrooms they forage. Cholanaikkans, named “Cavemen of Kerala,” dwell in Nilambur Valley’s forest. Living in rock shelters known as “aale”, their unique identity emerges from cave-named distinctions, adding -nu to names. Their distinct identity, denoted by cave-assigned names, remained hidden till 1971; gaining notice in 1977. With just 176 in number, mingling with Kattunaickan and Pathinayakkan tribes, this underscores the need for further research to unveil their story.

Centuries of wisdom passed down through generations, have granted these tribes an innate understanding of the forest’s bounties. Their knowledge of the edibility and healing properties of mushrooms is a blend of folk taxonomy and indigenous lore. Yet, as modernity creeps in, this ancestral wisdom risks fading away. Auricularia auriculata, Agaricus bisporus, Boletus edulis, Ganoderma lucidum, Lentinus edodes, and L. squarrosulus – these names carry the weight of tradition, the echoes of ancient remedies. These medicinal mushroom species, once integral to the tribal communities, now stand at the precipice of being forgotten.

Diving deep into India’s cultural tapestry, we uncover a captivating realm of ethnomedicinal wealth – a repository of traditional mycological knowledge. Indian ethnic groups have embraced this ancient wisdom, harnessing the power of wild mushrooms for over 283 species out of the global tally of 2000. This journey dates back to antiquity, as the ancient medical treatise, Charaka Samhita (3000±500 BC), whispers tales of mushrooms’ dual role as sustenance and medicine.

The spotlight of ethnomycological exploration shines brightly upon India’s North-East. Among the vibrant tribes like Garos, Adivashis, Bodos, and Rajbangshis of Western Assam, seven mushroom species stand as cherished vegetables. G. lucidum emerges as an herbal hero, combatting ailments like asthma. In the mystic land of Sikkim, local folk healers craft herbal elixirs with cordyceps, a potent remedy for a spectrum of afflictions – from cancer to colds.

Venturing further, the tribal tapestry expands. Nagaland’s ethnic tribes infuse wild edible mushrooms into their culinary heritage. Down South, the Kaani tribes of the Kanyakumari district dance with flavors like P. sajor-caju, T. heimii, and more, embodying the fusion of taste and tradition. Amidst the verdant Similipal forest, tribal communities nourish themselves with Russula, Termitomyces, and Pleurotus – a feast that heals maladies and weaknesses.

The saga continues through the undulating landscapes of Assam, Manipur, and Arunachal Pradesh, where wild mushrooms reign as nutritional saviors. But as we immerse ourselves in this mycological saga, questions arise: What untold stories lie within the mushrooms of the Northeast? How have generations of tribal wisdom influenced their use? Can these remedies hold the keys to modern ailments? As we tread the path of ethnomedicinal exploration, India’s tribal heart reveals a profound synergy between nature and healing, poised to captivate curious minds and inspire modern medicine alike.

 

Road Ahead: for Preservation and Innovation around fungal diversity

If you’re reading this, kudos to you for thinking about growing your own mushrooms. I hope this blog gives you the confidence to take the next step and get started on your mushroom cultivation journey. This blog is for anyone who wants to learn mushroom cultivation at a hobby level. It has tried and tested cultivation methods to grow mushrooms which are relatively easy for someone who wants to start growing mushrooms at home. 

If you don’t have much experience in growing mushrooms, then I recommend you go through our earlier blog on what mushrooms are and another blog with commonly used terminologies in mushroom cultivation.

What are the advantages of mushroom growing?

Why farm mushrooms?  As a budding mushroom farmer, you need to be aware of some of the benefits of mushroom cultivation before we get into the details of how mushrooms grow. Here is a list of 6 advantages of farming mushrooms –

1. Growing mushrooms is sustainable: Mushroom farming uses agricultural waste such as paddy straw, wheat straw, ragi straw, rice bran, corn cobs, soya hulls etc as input material. By cultivating mushrooms we are able to use a lot of by-products of agriculture and turn them into high-quality nutrition. Whatever waste that is left behind can be added back into the soil or composted, making the process circular.
2. Mushroom production is very water efficient: Though mushrooms are 90% water, they only use a fraction of water to grow as compared to traditional crops.
3. Mushroom growing doesn’t require soil or sunlight: You don’t need outdoor space to grow your mushrooms and mushroom cultivation can be done indoors in minimal space. This also means that growing mushrooms at home doesn’t depend on soil fertility, pests, or other external factors which are hard to control outdoors.
4. Mushroom cultivation is vertically scalable: Mushroom beds or bags can be stacked vertically which means that small-scale mushroom farming can be done even if you only have a small room at home.
5. Mushrooms are a high-quality food source: Apart from macronutrients such as protein and fiber, mushrooms contain many unique bioactive molecules that can improve your health and well-being. They are a good source of amino acids, vitamins minerals, and even rare bio-compounds such as Beta Glucans, Terpenes, and Phenolics.
6. Cultivation of fungi or mushrooms takes relatively less time: Mushrooms are quite fast-growing when compared to plants. Our pink oyster mushroom is capable of giving you your first harvest in 3 weeks! Compared to traditional food crops you can get more harvests in a year when growing mushrooms which means more income and lower overhead costs per cycle.

If you want to dive deeper into more reasons why you should cultivate mushrooms, please check out the 3 part blog series on why India needs more mushroom farmers: Part1.

What is mushroom cultivation?

Contrary to what most people believe, the most amount of time and effort in growing mushrooms is actually devoted to growing mycelium and not the mushroom itself. Let me give you an example: To grow oyster mushrooms the first 6-8 weeks is actually spent in making spawn and allowing the mycelium to grow and colonize the substrate. The actual fruiting process where you grow mushrooms takes only 7-10 days. Think of this similar to growing fruit trees, most of the effort goes into growing the tree and taking care of it as compared to the actual fruit. Fruit trees usually take 5-10 years to mature and give you the first harvest and the actual fruits take roughly 2-3 months to grow. To get good fruits you need to pay attention to how the trees grow. Similarly, to get good mushrooms, you need to understand and take care of mycelium which then gives you healthy mushrooms under the right conditions.

In short, mushroom cultivation is the process of growing mushroom mycelium, expanding it, feeding it, and then maintaining the right conditions to trigger the growth of mushrooms. Mushrooms are essentially the fruiting bodies of the vegetative mycelium, which exist to spread spores. 

How are mushrooms grown?

There are 8 broad steps in mushroom cultivation-

1. Mycelium on Agar: A tiny piece of the mushroom tissue is placed on a nutritive medium called agar, in a petri plate. This process is called tissue culturing. Slowly, over the next week or two the mycelium expands and grows over the entire agar surface, “colonizing” it. Here the agar serves two purposes- a 2D surface for the mycelium to expand and also the nutrition for the mycelium to feed on. A fully colonized plate of mushroom mycelium is also called mushroom culture.
   
   
2. Spawn production: Once the petri plate is fully colonized by mycelium, a tiny piece of mycelium is transferred to a container filled with half boiled grain or sawdust. The surface of the grain or sawdust provides more surface area for the mycelium to spread. The water and nutrients inside the grain/sawdust ensure the mycelium stays alive and healthy. Spawn helps the cultivator by providing more points for the mycelium to start growing from compared to a single point. If you want to learn more about spawn and how it is made, please go through our earlier Mushroom Spawn 101 blog. The entire process mentioned in steps 1 & 2 is usually carried out in a lab or clean room. Sterile protocols are followed and all the work happens inside a Laminar Airflow or Biosafety Cabinet. Both these devices provide a smooth stream of HEPA-filtered clean air to work in, lowering the chance of contamination. This is why spawn production and mushroom culture preparation is considered expensive and difficult.
3. Prepping the substrate: Any material that you use to grow mushrooms on needs to be prepped beforehand. Prepping can include multiple steps depending on what substrate you choose. For example, if you’re working with paddy or wheat straw, steps include chopping it up into 1-3” pieces and then soaking in water to hydrate it.
4. Cleaning of substrate: Any material (substrate) that you intend to grow mushrooms on needs to be cleaned before you use it. This is done to reduce the chances of contamination by killing off competing microorganisms present in the substrate. There are many ways to clean your substrate including steam/heat pasteurization, lime pasteurization, sterilization in an autoclave etc. You need to choose an appropriate method on the basis of your substrate material and resource constraints.
5. Inoculation of substrate with spawn: After the substrate has been prepped and cleaned, spawn is added to it. This step needs to be done in a clean space and depending on what mushroom you are growing and the kind of substrate you are working with, you might have to invest in some equipment for the same.
6. Incubation of inoculated substrate: During this stage, the mycelium slowly starts growing and spreading across the substrate. Depending on the species this step can take anywhere between 3 weeks to 2 months. The bags are allowed to incubate undisturbed in a designated area where the temperature and light conditions are optimal for the growth of mycelium.
   
7. Fruiting myceliated substrate: Once the bags are fully colonized with mycelium, they are shifted to a location where the conditions are optimal to trigger fruit body production. Specific fruiting conditions are dependent on the mushrooms being cultivated but it generally means maintaining ideal levels of temperature, humidity, light and fresh air.
   
8. Harvesting mature mushrooms: Once the mushrooms start pinning, they grow quickly and reach maturity very soon. The last stage is harvesting the mushroom at the ideal stage of growth. Mushrooms are usually harvested before they drop spores and timely harvest can influence the taste, texture, and shelf life of mushrooms.

 

How do I get started?

Before you begin your journey of growing mushrooms at home, you need to think about a very important question- Which mushroom am I going to grow? 

Let’s look at this question in a little more detail-

Deciding which mushroom you will grow is a really critical decision in mushroom cultivation, if not the most important one. Your choice of mushroom will have an effect on-

1. Growing conditions: The mushroom you choose should be suitable to grow well given your local weather conditions. If you want to grow exotic mushrooms such as Lion’s mane, you might have to invest in an AC room dedicated to growing them which might turn out to be an expensive affair. Different mushrooms have very specific temperature, humidity, fresh air and light requirements. Please make sure you do a bit of research on the fruiting conditions of your strain before you go deeper into growing it.
2. Choice of substrate: Each mushroom has its own preference for what medium it wants to grow on. Most oyster mushrooms tend to do very well on paddy/wheat/ragi straw as compared to mushrooms such as button or portobello which prefer compost-based substrates. One of the major challenges as a home cultivator will be finding a reliable local source for substrate. So, make sure you choose mushrooms which you can grow using locally available materials so that the overall process remains sustainable.
3. Choice of substrate cleaning method: Once you have narrowed down on the substrate that you want to use for your mushroom, you will have to choose which method of substrate cleaning will work best for your needs. Substrates which are relatively low in nitrogen such as cardboard and straw can be boiled or even cold pasteurized using hydrated lime. Others such as master’s mix (hardwood sawdust + soy hulls) need to be sterilized using an autoclave at 121 degrees Celsius and 15 PSI pressure.

Please do note that the fruiting temperatures can change depending on the strain for the same species. For example we have seen strains of Shiitake mushroom which can fruit at 24 degrees Celsius and some which fruit only below 16 degrees Celsius

What substrate do I use?

Once you have chosen your mushroom, the next question to address is what material am I going to use as substrate to grow my mushrooms?

When evaluating substrate here are a few things you need to keep in mind:

1. Economical: Your substrate material should be affordable. It doesn’t make economic sense to grow your mushrooms using really expensive material as substrate
2. Easily Available: Use materials that can be sourced locally or can be procured online with ease.
3. Right nutrients: Substrates should have the right ratio of Carbon and Nitrogen to support the growth of mushrooms. Most agricultural waste that are rich in lignin, cellulose and hemicellulose make good substrates but you need to ensure that any material which is very high in nitrogen content is better suited as a supplement and should not be used exclusively as substrate. Example: spent coffee grounds, rice/wheat bran, soy hulls are very high in nitrogen and are best used as an additive to other substrates which are high in carbon such as sawdust, cardboard or straw.
4. Hydration capacity: A good substrate should be able to hold 50-70% moisture by weight. This water is what the mycelium will use to grow. A good way to know if a substrate is hydrated to the right amount is by doing a squeeze test- when you squeeze a fist full of hydrated material it should feel wet without the water dripping down in a stream. Different substrates can absorb different amounts of water so ensure you check the hydration capacity of the material before you choose to use it as a substrate.
   Here is a simple formula for you to check the hydration% of your substrate:Hydration % = [ [(Wet weight of substrate after hydration) – (Dry weight of substrate before hydration)] ÷ (Wet weight of substrate after hydration) ] × 100
5. Aeration: Your substrate should allow airflow so that the mycelium can breathe. Lack of airflow or poor airflow leads to anaerobic conditions which can promote the growth of bacteria leading to contamination. Any substrate can get anaerobic if it gets overhydrated or too compacted while packing it.
6. Free from competing organisms: Substrates you use should not have other organisms growing on it as this can lead to competition with mushroom mycelium ultimately resulting in poor or no yields. This happens because the mycelium ends up spending all its energy on defending itself against competition rather than producing mushrooms.

If you’re new to mushroom cultivation, we recommend using hardwood fuel pellets as substrate to begin with. These are sawdust which has been heat treated and extruded into the shape of pellets. They come pre-pasteurized and therefore the chances of contamination while using them are very low compared to sawdust procured from mills or even straw. We have sawdust substrate pellets available on our website for home use.

What method of substrate cleaning should I use?

Now that you have an idea of what substrate you will be using, the next thing that you need to decide is how you’re going to clean your substrate. There are plenty of ways to do this depending on your scale and budget. I will list down the 6 most common ways and how to perform them.

1. Steam Pasteurization:
   Suitable substrates: Straw, sawdust, cardboard, agri-waste such as shredded banana leaves, bamboo leaves time taken: 2-8 Hours
   1. Pass steam generated in a boiler to a chamber with hydrated substrate.
   2. Continue to pass steam and ensure the temperature is maintained at 60-65⁰ C for 4 hours or at 80-85⁰ C for 2 hours for straw or other agri-waste. In case you are using sawdust, you will need to pasteurize for 6-8 hours at 80-85⁰ C.
   3. Allow bags to cool overnight before inoculation.
      
2. Hot Water Pasteurization
   Suitable substrates: Straw, cardboard, agri-waste such as shredded banana leaves, bamboo leaves etc. Time taken: 2 Hours
   1. Plunge dry/hydrated substrate placed in a sack for 2 hours in hot water maintained at 80-85⁰ C for 2 hours.
   2. Remove the sack and allow it to cool and release excess water.
   3.  Note that the same water can be used 2 times, after which it needs to be discarded.
   4.  If you’re using cardboard, a quick dip in hot water at 80-85⁰ C should suffice.
      
3. Lime Pasteurization
   Suitable substrates: Straw, cardboard, agri-waste such as shredded banana leaves, bamboo leaves etc Time taken: 12-20 Hours
   1. Add hydrated lime (Ca[OH]₂) at a rate of around 2-2.5 g / Litre of water to 20 L
   2. 1.5 Kg of straw is then bagged in a sack and submerged in this water for 12-20 hours. (Keep a heavy object to make sure straw stays submerged)
   3. After soaking, the straw is drained well to remove excess water and then inoculated as normal. 
   4. Excess lime water can be used for subsequent treatment batches, just be sure to add more lime as needed to maintain the proper pH (12-13).
      
4. Cold Fermentation
   Suitable substrates: Straw Time taken: 7-14 days
   1. Submerge the straw in a drum filled with water for 7-14 days.
   2. The substrate will smell somewhat foul and the water surface will be covered in a thin layer of slime. This is good; it means that the fermentation was successful. 
   3. The substrate is then removed and suspended above the fermentation tank to drain until water stops dripping from the substrate.
5. Ash Pasteurization
   Suitable substrates: Straw, wood ash, cardboard, agri-waste such as shredded banana leaves, bamboo leaves etc. Time taken: 12-20 Hours
   1. Add Wood Ash at a rate of around 20g / Litre of water to 20L.
   2. Use a PH strip to ensure the PH is more than 10. If it is lower, add more ash.
   3. 1.6 Kg of straw is then bagged in a sack and submerged in this water for 12-20 hours. (Keep a heavy object to make sure straw stays submerged)
   4. After soaking, the straw is drained well and then inoculated as normal.
6. Autoclave/Pressure Cooker Sterilization
   Suitable substrates: ALL- hydrated substrates. Time taken: 30 – 180 Minutes (30 minutes for straw, 2-3 hours for nutrified sawdust)
   1. Put enough water in the bottom of the PC so that by the end of the pressure cooking there is still some water left at the bottom of the PC. For most runs, 0.5 inches (1.25 cm) of water is sufficient. Never run the pressure cooker dry!
   2. Place your materials in the PC and securely close the lid. If using a “rocker top” pressure release system, leave the weight off the vent port. If using a “petcock” pressure release, open the petcock. Turn the heat source to a level that requires 15 minutes to pass before steam begins to flow from the petcock or vent port. Heating the PC too rapidly can cause substrate bags to break.
   3. Allow a steady jet of steam to escape from the pressure release vent for 1-5 minutes and then place the weight on the vent port or close the petcock. This ensures that everything heats evenly. The PC will quickly come up to pressure in 5-15 minutes. Once the desired pressure is reached (typically 15 psi), reduce the heat level on the burner until the pressure stabilizes. It may take a few minutes to adjust the heat to get it just right. If using a “rocker top;’ adjust the heat source so that the rocker maintains a slow, steady rocking motion and/or jiggles once a minute or so.
   4. Once the pressure is stabilized, start your timer. You will need to sit with the pressure cooker during the entire run to make sure the pressure remains constant and to adjust the heat accordingly. Cook your materials for the specified run time. If you are above 2000 feet (6000 m) in elevation you will need to add 5% to the cooking time for every 1,000 feet (300 m) (i.e. at 3,000 feet [900 m] add 5%, 4,000 feet (1,200 m] at 10%, etc.).
   5. When the run time is over, turn off the heat source and walk away to let the PC cool and de-pressurize on its own. I do much of my PC work at night so that everything is cooled by the following morning.

      

 

Choosing between sterilization and pasteurization:

Both sterilization and pasteurization have pros and cons, as a cultivator you will have to choose one of the methods based on your constraints and requirements. Here are some fundamental differences between both approaches:

 

5 Really easy ways to grow mushrooms at home

Here are 5 of the easiest and simplest ways to grow mushrooms at home. Please note that most of these methods are geared towards the cultivation of Oyster mushrooms as they are really aggressive and less prone to contamination. If you want to learn how to grow mushrooms like shiitake and lion’s mane you will need to use an autoclave or steam sterilizer. They also need to be inoculated under sterile conditions using a Laminar Air Flow or a Still Air Box.

There are many other ways to cultivate mushrooms out there but I find these 5 methods to be the simplest- 

Method 1: Use a mushroom-growing kit

This is the easiest way to grow mushrooms. The best part about growing kits is that they come pre-inoculated with spawn and fully colonized. You can proceed to grow mushrooms from day one without waiting. There are many advantages in starting your home cultivation of mushrooms using growing kits-

1. No risk of contamination: As the kits come pre-inoculated and colonized, the risk of contamination is eliminated.
2. Saves time: The kit is ready to use from day 1 and you can save a lot of time (2-6 weeks) that would have otherwise gone into prep and incubation.
3. Very convenient: The kits usually come with all materials and instructions included which means that you don’t need to spend time or effort in procuring any extra materials.
4. Customer support: At Nuvedo we ensure that we help each and every one of our customers in their mushroom-growing journey. We have a support team who will help you troubleshoot and answer any queries you may have while you grow your mushrooms. Before you get started make sure you select the mushroom which is best suited for your location. At Nuvedo We offer many different kits for home use including white, pink, and golden oyster mushrooms and even Lion’s mane. If you’re unsure which kit is suitable for your location please use this widget and enter the temperature and humidity at your location, the widget will then suggest the best kits for your location. What you need: Any fully colonized mushroom growing kit with a species of mushroom suitable for your location.
   Time taken: 5 minutesInstructions:
   1. Find a good location to place your kit. Make sure it is clean, free from pests, has ambient lighting and enough fresh air.
   2. Cut a small  2-3 inch ‘X’ mark on the plastic cover.
   3. Mist with drinking water regularly (Make sure you don’t spray directly on the mushrooms themselves)
   4. Watch your mushrooms grow!
   5. Harvest the mushrooms when they’re ready!

 

Method 2: Bucket Tek using straw

This technique is quite popular with both home growers and commercial cultivators and also eco-friendly since you avoid using single-use plastic completely. Here you use a reusable food-grade plastic bucket or container to house your substrate while the mycelium feeds on it. We will be using the lime pasteurization method to clean your substrate. Please note that this method of cultivation is best suited for oyster mushrooms.

What you need: 10L Paint bucket or food grade plastic container (PP5 is a really good plastic to use), 900g-1Kg straw, low magnesium hydrated lime powder or NuvoPast, scissors, micropore tape, isopropyl alcohol or any disinfectant of your choice. Our DIY Mushroom Growing Kit comes with most of the materials mentioned above including an information pamphlet and YouTube video which explains the entire process.

Time taken: 90-120 minutes

Instructions:

STEP 1: Cleaning and hydrating your substrate.

1. Put 1 Kg chopped straw into a meshed bag / cloth bag / pillow case
2. Fill a 20 liter bucket with water, leaving 2-3 inches gap from the top of the bucket
3. Using a weighing scale, measure 25-30 grams of hydrated lime powder
4. Mix the powder into the water thoroughly
5. Put the substrate-filled bag into the bucket and place a heavy object on top to make sure that all of your substrate is submerged under water
6. Leave the substrate bag submerged for 12-20 hours

 

 

STEP 2: Preparing the bucket

1. Make 3-5 x 8mm diameter holes on the bucket as shown below. These holes are where the mushrooms will grow from
2. Make 4 x 3mm holes at the bottom. This is for excess water to drain out of the substrate
3. Clean the bucket and lid thoroughly with isopropyl alcohol or soap solution
4. Tape all holes on the side wall of the bucket (not the holes below), with 2 layers of micropore tape. This is to ensure that no contaminants enter the bucket through these holes.

   

 

STEP 3: Inoculation

1. Remove the bag with substrate from the bucket
2. Squeeze the bag to remove excess water
3. Take a fistful of substrate and squeeze it. The straw should feel wet without any water dripping out of it. If the straw feels wet, it needs to be dried more before use
4. Take the sanitized bucket and pack the bottom with a 5-6 cm thick layer of substrate
5. Using the spawn packet provided, make an even thin layer of spawn above the substrate layer
6. Alternate with layers of substrate and spawn and top off the bucket with a final layer of substrate
7. Pack everything in and close the lid.

Your mushroom growing bucket is now ready for incubation!

 

STEP 4: Incubation

Leave your bucket in a cool, dry and hygienic place away from direct sunlight, for a period of 2-3 weeks. The mycelium slowly grows on the substrate and breaks it down to feed itself, over the next few weeks.

STEP 5: Inspection & Fruiting

1. After the 3 week mark, open the bucket lid. The inside should be almost completely covered in white mycelium.
2. If you can still see straw which has not been colonized, close the bucket and check it after a few days
3. If you notice any discoloration such as green, blue or pink, this means that your substrate has been contaminated. You will have to empty the bucket, clean thoroughly and start again
4. Once your substrate has been completely colonized, open the micropore tape. This increases the airflow, triggering the mycelium to produce pins
5. Spray the bucket with clean drinking water 2-3 times daily
6. Watch the mushrooms grow!
7. Harvest them at the right time to enjoy your first flush of oyster mushrooms!

   

 

 

 

 

 

Method 3: Using hot water pasteurized sawdust pellets

In my opinion, this has to be the easiest and most foolproof way to grow mushrooms at home. You can use this method to grow all varieties of oysters and even some hardy medicinal varieties such as Reishi and Turkey tail (since they are aggressive colonizers). Other mushrooms such as shiitake, lion’s mane, enoki, etc can be grown on plain sawdust pellets but they need additional supplements such as soy hulls. Nutrified sawdust needs to be autoclaved to sterilize it well and they can only be inoculated inside a Laminar Air Flow or a Still Air Box which makes them hard to grow for beginners.

What you need: PP5 bag with breathable filter, 1 kg sawdust pellet, hot water, scissors, isopropyl alcohol or any disinfectant of your choice, and tape. We have a DIY Mushroom Cultivation Kit which comes with all the inputs you need including a detailed instruction pamphlet that describes the process in depth! 

Time taken: 6-7 hours

 

STEP 1: Pasteurizing the substrate

1. Add EXACTLY 1.5 Liters of boiling hot water inside the filter patch bag containing 1 Kg substrate pellets. This helps in pasteurizing and hydrating the substrate pellets
2. Fold the top of the bag and tape it down with a piece of cello tape.
3. Wait for 5-6 hours while the bag cools down to room temperature.

STEP 2: Inoculation with grain spawn

1. Sanitize your hands, scissors and work space with soap water/Iso propyl alcohol or any other disinfectant
2. Break up the spawn inside the packet and then cut open the packet using your scissors
3. Remove the tape on the cooled bag of substrate and add the spawn into it
4. Fold the top of the bag over itself and tape it shut using cello tape. Ensure that no air can enter or leave the bag as it can lead to contamination
5. Mix the spawn into the substrate from outside the bag using your hands

 

STEP 3: Incubation

1. Move the bag to a cool, clean area away from direct sunlight and let it incubate for 15-21 days.

STEP 4: Fruiting

1. Once the mycelium has completely colonized the bag, you can proceed to cut an X mark on the bag using a sharp knife or a blade.
2. Spray clean, drinking water using the spray bottle provided on the plastic thrice a day
3. Once the mushroom pins emerge, let them grow till they reach maximum size. Twist and pull gently to harvest!

Method 4: Hot water pasteurized straw in plastic bags

This method is the most commonly used method to cultivate mushrooms in India. Most mushroom farms in India who grow Oyster mushrooms tend to boil their straw in order to pasteurize it. This is a fairly simple method but the only downside is that you need to have a heat source and a container large enough to fit 1kg of chopped straw.

What you need: Plastic bag (preferably food grade) , 1Kg straw, scissors, micropore tape, isopropyl alcohol or any disinfectant of your choice, stove/induction cooker, needle or safety pin

Time taken: 2-3 hours

Instructions:

STEP 1: Cleaning and hydrating your substrate.

1. Put 1 Kg chopped straw into a meshed bag / cloth bag / pillow case
2. Fill a 20 liter metallic vessel with water, leaving 2-3 inches gap from the top of the bucket
3. Put the substrate filled bag into the bucket and place a heavy object on top to make sure that all of your substrate is submerged under water
4. Turn on the stove/hotplate and put it on high heat to bring the water to a boil
5. Reduce the heat so that the temperature is maintained above 80 degrees
6. Leave the substrate bag submerged for 2 hours

Image- https://cdn.bitlanders.com/users/galleries/315133/315133_gallery_551542dd62ea2_jpg_fa_rszd.jpg

 

STEP 2: Inoculation

1. Remove the bag with substrate from the bucket and let it dry for 1-2 hours in a clean area.
2. Squeeze the bag to remove excess water
3. Take a fistful of substrate and squeeze it. The straw should feel wet without any water dripping out of it. If the straw feels wet, it needs to be dried more before use
4. If the spawn is clumped up, break it up into smaller pieces from the outside
5. Take the plastic bag and pack the bottom with a 5-6 cm thick layer of substrate
6. Use the crumbled spawn packet provided, make an even thin layer of spawn above the substrate layer
7. Alternate with layers of substrate and spawn and top off the bag with a final layer of substrate
8. Pack everything in and seal the top by folding it and taping it shut
9. Using a small needle, poke 15-20 tiny holes around the bag (for best results we recommend you use a filter patch bag and heat seal it to prevent any contamination but for home cultivation of oyster mushrooms the technique mentioned above will work)

Your mushroom growing bag is now ready for incubation!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

STEP 4: Incubation

Leave your bag in a cool, dry and hygienic place away from direct sunlight, for a period of 2-3 weeks. The mycelium slowly grows on the substrate and breaks it down to feed itself, over the next few weeks.

STEP 5: Inspection & Fruiting

1. After the 3-week mark, check the bag. The entire bag should be covered with white mycelium
2. If you can still see large patches of straw which has not been colonized, let it incubate undisturbed for a few more days.
3. If you notice any discoloration such as green, blue, or pink, this means that your substrate has been contaminated. You will have to discard the bag and start again
4. Once your substrate has been completely colonized, use a pair of clean and santised scissors (Use soap water or Isopropyl alcohol to clean the blades) to cut open an X-shaped slit on the bag. This increases the airflow, triggering the mycelium to produce pins
5. Spray the bag with clean drinking water 2-3 times daily
6. Watch the mushrooms grow!

Harvest them at the right time to enjoy your first flush of oyster mushrooms!

Method 5: Lime pasteurized sawdust pellets

This is another method of mushroom cultivation that makes use of sawdust pellets and is the same as hot pasteurization of sawdust pellets apart from one difference. The difference here is that you will be using hydrated lime powder to pasteurize your substrate instead of boiling hot water. This method doesn’t need any heating and can be done very easily at home. This is different from Bucket Tek since you don’t need to spend much time soaking or drying the substrate. The waiting time is much less- 3 hours compared to 6 hours of hot pasteurization and 12 hours of lime pasteurized straw in buckets.

What you need: PP5 bag with breathable filter, 1Kg sawdust pellets, low magnesium hydrated lime powder OR NuvoPast, scissors, isopropyl alcohol or any disinfectant of your choice, tape

Time taken: 90-120 minutes

STEP 1: Pasteurizing the substrate

1. Add 2 heaped tablespoons of Pasteurization powder into EXACTLY 1.5 Liters of drinking water and mix well. Add this water to the filter patch bag containing 1 Kg substrate pellets. 
2. Fold the top of the bag and tape it down with a piece of cello tape.
3. Wait for 3 hours for the pellets to get hydrated and then mix the pellets and water mixture by applying pressure on the outside of the bag using your hands.

STEP 2: Inoculation with grain spawn

1. Sanitize your hands, scissors and work space with soap water/Isopropyl alcohol or any other disinfectant
2. Break up the spawn inside the packet and then cut open the packet using your scissors
3. Remove the tape on the cooled bag of substrate and add the spawn into it
4. Fold the top of the bag over itself and tape it shut using cello tape. Ensure that no air can enter or leave the bag as it can lead to contamination
5. Mix the spawn into the substrate from outside the bag using your hands
6. Move the bag to a cool, clean area away from direct sunlight and let it incubate for 15-21 days

STEP 3: Incubation

1. Once the mycelium has completely colonized the bag, you can proceed to cut an X mark on the bag using a sharp knife or a blade.

STEP 4: Fruiting

1. Spray clean, drinking water using the spray bottle provided on the plastic thrice a day
2. Once the mushroom pins emerge, let them grow till they reach maximum size. Twist and pull gently to harvest!

What is the best way to fruit mushrooms?

Once you’re done with incubating your substrate bags, the next obvious question is how do I go about fruiting it? Well, each mushroom species has a specific fruiting condition in which it does best so it is important to ask your spawn vendor what the right conditions are for the strain they have provided. 

Fruiting conditions mean 4 parameters- Temperature, Humidity, CO2 levels and Light levels.

The fruiting room should be clean and hygienic to start with. The room should have ample ventilation to ensure fresh air exchange. There should be enough light in the room- a window that allows sunlight during the day or even a lightbulb will do. Make sure the bulb doesn’t make the room too hot (unless you live in a place where temperatures are very low). As for the humidity, most mushrooms thrive in RH of >80% but anything above 60% will do. If you live in a really dry area you can try one of these hacks or you can consider investing in a small humidifier. Temperatures are hard to control without an Air Handling Unit or AC so please make sure you’re growing mushrooms that are suitable for your local microclimate. Please note that ACs tend to de-humidify the room, so in case you are using one make sure the humidifier is not directly in line with the flow of air from the AC! 

All the conditions mentioned above become critical when you’re cultivating mushrooms for commercial use at a slightly bigger scale. As a home user, you can get away with using a humidity tent or wrapping a wet towel around the base of the kit.

 

In closing, thank you for taking this journey through the world of mushroom cultivation with me. Your curiosity and interest have already set you on the path to a rewarding and exciting venture. Remember, as with any new endeavor, you might encounter challenges along the way, but rest assured, the satisfaction and joy derived from growing your own mushrooms will far outweigh any obstacles.

Should you find yourself with questions or seeking further clarification on any aspects of this guide, please don’t hesitate to comment below. Your insights, experiences, and queries enrich our community of mushroom enthusiasts and may very well inspire my next blog post.

Always keep in mind that mushroom cultivation, like any horticultural activity, is as much an art as it is a science. There isn’t a ‘one-size-fits-all’ approach, so I urge you to experiment and discover the methods and techniques that resonate best with your own unique situation. This blog is simply a stepping stone, a launchpad to your personal adventure in the realm of fungi.

Lastly, remember that the journey is just as important, if not more so, than the destination. Stay motivated, stay curious, and most importantly, enjoy every moment of the process. This journey of mushroom cultivation promises a world of discovery and I’m excited to hear about your triumphs and learnings along the way.

May your home teem with bountiful fungi and your spirit be enriched by the process. Happy mushrooming!

Beta Glucans: What are they really?

Mushroom extracts are all the craze today. Have you ever wondered what are in these extracts? What do you as a consumer look out for when you buy a product? The answer to these questions is not very simple as there are an array of different bioactive molecules which give mushrooms their functional benefits. The three main classes of biomolecules being- polysaccharides, terpenes and phenolics.  In this blog we will delve deeper into the world of polysaccharides, an important class of compounds that are responsible for giving mushrooms a lot of their healing properties. However, please do keep in mind that all polysaccharides are not the same, as we will see later in this blog. Our primary focus here will be Beta glucans, which are the main functional components of mushroom polysaccharides. 

In our previous blog we had a look at the intricacies of the fungal cell wall. The three major layers (Chitin, beta glucans and mannoproteins) are intertwined into a dynamic barrier that has stood the tests of time and allowed fungi to thrive (See Figure 1). Having evolved with fungi for billions of years, our immune systems recognize these cell wall components as a threat and spring into action. These molecules are what give mushroom extracts their immuno-modulatory function. A major active ingredient in these extracts are the beta glucans. They are polysaccharides that form the middle layer of the fungal cell wall (Layer 2 in Fig 1). 

 

 

Fig 1: Cartoon representation of a Candida albicans (yeast) cell wall (Source)

 

Beta glucans are incredibly diverse in their composition and function. Different mushrooms contain different beta glucan molecules. To fully understand these differences, we will be exploring these polysaccharides from a molecular perspective. 

In this blog we will deconstruct the polysaccharide molecule and understand how they are named. We will also explore some of the common beta glucans obtained from mushrooms and their health benefits. By the end of this blog we hope that you will have a much better understanding of what beta glucans are and what to look for in your own research.

Before we delve into the details of beta glucans, we must first understand the term polysaccharide.

What are Polysaccharides

Any molecule composed of long chains of carbohydrates strung together is known as a polysaccharide (“poly” = ‘Many’, “Saccharide” = ‘Sugar’). These chains are made of sugars called monosaccharides. The most common examples of monosaccharides are glucose, mannose, fructose, galactose and xylose. Our beloved table sugar is in fact made up of glucose and fructose. Each molecule of sugar (sucrose) consists of one glucose and one fructose molecule. Since there are two monosaccharides in sucrose, it is known as a disaccharide. When the number of monosaccharides exceeds twelve the molecule is known as a polysaccharide. 

Fig 2: Sucrose, a disaccharide made of D-Glucose and D-Fructose

 

Glucose: The building blocks of beta glucans

To understand how polysaccharides are named, we must first look at the molecular structure of a polysaccharide molecule. We start with the humble glucose molecule. Glucose is nature’s preferred fuel source. Almost all living things break down glucose within their cells to generate ATP (adenosine triphosphate) which is the energy source for biochemical reactions. 

Structure of a Glucose Molecule

Glucose consists of 6 carbon atoms, 12 hydrogen atoms and 6 oxygen atoms giving it the molecular formula of C₆H₁₂O₆. The carbon atoms are arranged in a 6-member chain with the oxygen and hydrogen atoms sticking out from the central chain. Every carbon atom is attached to at least one hydrogen atom and either an alcohol (-OH) or ketone (=O) functional group. The distribution of these functional groups determines the name of the monosaccharide. Glucose is specifically the 6- carbon monosaccharide which has an arrangement of atoms as depicted in the figure below.

Fig 3: Structure of a D-glucose molecule in the open-chain format

 

Many Forms of Glucose

If one were to change the orientation of the -OH and -H molecules on any of the carbon atoms, we would obtain a different monosaccharide. For example, galactose and mannose have the same number of carbon, oxygen and hydrogen molecules as glucose but with different arrangements. Such molecules are known as isomers. Hence, mannose and galactose are isomers of glucose.

Fig 4: Isomers of D-glucose depicted in the open-chain format highlighting the distribution of functional groups across each molecule

 

The Ring of Power

In reality however, these monosaccharide molecules do not exist as open- chains. The first and fifth carbon atoms in the chain fold over to form a hexagonal structure with the oxygen atom forming a bridge between these carbon atoms. This is the functional form of the D-glucose molecule. 

Fig 5: The folding of an open-chain glucose molecule to a hexagonal glucopyranose unit

 

What’s in a Name?

Glycosidic Bonding

Now that we have a basic understanding of the structure of the monosaccharides, we can begin to understand how they link together to form polysaccharides. The bonds that link these monosaccharide molecules to one another are called glycosidic linkages or glycosidic bonds. These linkages are traditionally formed between the alcohol (-OH) containing first, third, fourth or sixth carbon atoms. So when a bond is called a 1 → 4 glycosidic bond, it indicates that the first and fourth carbon atoms of adjacent molecules are participating in the bond formation. 

Alpha or Beta?

Another important aspect of the bond is its orientation. The roman letter alpha (𝛼) or beta (𝛽) are used to indicate this orientation. This can be better understood by considering the two disaccharides in the image below. Let us begin by imagining the ring to be a plane with the functional groups sticking out above and below this plane.

Figure 6 a) represents the link formed between adjacent D-glucose molecules in a Maltose molecule. The first and fourth carbon atoms of the adjacent glucose molecules partake in a 1→ 4 glycosidic bond. The (-OH) groups taking place in the bond lie on the same plane below the hexagonal ring structure. Bonds taking place in the same plane are termed 𝛼-glycosidic bonds. Therefore Maltose exhibits an 𝛼 (1→4) glycosidic bond between its two glucose monosaccharides.

In contrast Lactose molecules (Fig 6 b)) are linked through 𝛽 (1→4) glycosidic bonds. The (-OH) molecules from galactose and glucose that take place in this bond formation lie on opposite sides of the hexagonal ring structure. This bond therefore forms across this plane. 

Fig 6: a) The combination of two D-glucose molecules via an 𝛼 (1→4) glycosidic bond results in the formation of a Maltose molecule

b) The combination of D-galactose and D-glucose molecules via a 𝛽 (1→4) glycosidic bond results in the formation of a Lactose molecule

While this terminology can be rather confusing, the important thing to remember is that 𝛼 glycosidic bonds form on the same plane while 𝛽 glycosidic bonds form across two different planes. This difference contributes to very different three-dimensional conformations for the two kinds of bonds. 𝛼 bonds are more linear resulting in tightly packed structures as in the case of amylose (a component of cellulose), while 𝛽 bonds are more flexible and tend to rotate into helical shapes like in the case of chitin. 

Different kinds of beta glucans

The above examples demonstrate the different kinds of bonds that can form between monosaccharide molecules. However, when we talk about bioactive polysaccharides from mushrooms, we are specifically interested in beta glucans. 

Mushroom beta glucans are those polysaccharides that consist of glucose molecules linked together via 𝛽 (1→3) and 𝛽 (1→6) glycosidic bonds. This is an important distinguishing factor since beta glucans are also found in a wide variety of plant foods such as barley and oats.

These plant beta glucans consist primarily of 𝛽 (1→3) and 𝛽 (1→4) glycosidic linkages. The 𝛽 (1→4) linkages result in a more linear and packed structure. As a result, these plant glucans are harder to break down in our digestive tracts. They thus contribute to the beneficial insoluble fibers that nourish our microbiome.

Beta glucan diversity

Beta glucans are found in all fungi, including yeasts. Different fungi have different glucan compositions. Ascomycete fungi such as Cordyceps contain glucans that have varying amounts of glucose, galactose and mannose. Basidiomycete fungi such as oyster mushrooms, Reishi, Turkey tail and Shiitake have predominantly glucose in their glucan molecules with minor contributions from the other monosaccharides.

The ratios in which the different monosaccharides are found in the glucan molecules affect both their structures as well as their bioactivities. The different configurations and branching structures of beta glucans lead to differences in their effects on our health. These effects vary considerably from species to species. It is therefore not surprising that the species of mushrooms that have been used for medicinal purposes for the last two to three thousand years are also the ones which have the highest beta glucan content. 

We always recommend our readers to look for beta glucan content in their mushroom extracts. Polysaccharide content does not imply beta glucans.

Common sources of beta glucans and their health benefits

If you’ve ever consumed a mushroom, you have consumed beta glucans. Many of the health benefits of consuming mushrooms come from the beta glucans. As beta glucans are an essential part of the fungal cell wall (see blog), every cell of the mushroom contains beta glucans. This makes them very easy to incorporate into our diets. All we have to do is consume mushrooms, although some ways of consumption lead to better absorption compared to others.

Oyster mushrooms 

Oyster mushrooms are robust and easy to grow. They are a beginner friendly mushroom to try growing at home. They grow happily on almost any plant based substrate and are ready to burst out fruiting within a few weeks. (if you want to grow your own at home try our mushroom growing kits)

Fig 7: Pleurotus ostreatus (Pearl oyster mushroom) [image illustration]

Pleuran is a beta glucan isolated from Pleurotus ostreatus (Pearl Oyster Mushroom). It has been shown to have an immunomodulatory effect while also being beneficial for skin, gut and respiratory health. It also has antiviral activity.

Fig 8: Molecular structure of Pleuran, a 𝛽 glucan extracted from oyster mushrooms (Source)

Shiitake Mushroom

Shiitake mushrooms are another edible variety that is rich in beta glucans. It has long been consumed by cultures of the far East and is revered both for its culinary and medicinal properties. 

Fig 9: Lentinula edodes (Shiitake mushroom) [image illustration]

Lentinan is a beta glucan isolated from Lentinus edodes (Shiitake Mushroom). It has  potent immunomodulatory effects. This in turn promotes the body’s own anti-cancer, antiviral and antimicrobial abilities. It is one of the most well understood beta glucans. Its widespread use is restricted by the difficulty of extraction of this molecule.

Fig 10: Molecular structure of Lentinan, a 𝛽 glucan extracted from shiitake mushrooms (source)

Maitake Mushroom

The Hen of the woods, also known as Maitake in Japan, is well known for its delicious taste and aroma. However it is also rich in beta glucans that provide immunomodulatory, antidiabetic. antiviral and antitumor properties.

Fig 11: Grifola frondosa (Maitake mushroom) [image illustration]

 

Grifolan is a beta glucan isolated from Grifola frondosa (Hen of the Woods mushroom). It has strong antitumor activity.

Reishi Mushroom

Apart from edible varieties, most medicinal mushrooms are particularly rich in beta glucans. The Reishi mushroom, also known as the herb of immortality in traditional Chinese medicine, has a very high beta glucan content. These beta glucans are in part responsible for the legendary immunomodulatory properties of Reishi mushroom extracts.

Fig 11: Ganoderma lucidum (Reishi mushroom) [image illustration]

Ganoderan is a beta glucan isolated from Ganoderma lucidum (Reishi mushroom). It possesses antitumor, hypoglycemic, antioxidant, probiotic and immunomodulatory properties. 

Conclusion

Table 1: List of mushrooms with their beta glucans and associated health benefits

In this article we delved into the structural details of beta glucans. We took apart the differences between polysaccharides and beta glucans and looked at how the glucose molecule forms the fundamental building block for most beta glucan molecules of fungal origin. Finally, we explored some of the commonly studied beta glucans and the mushrooms they came from. 

Beta glucans are extremely diverse in their structure. No two mushroom species will have the same beta glucan composition. These differences allow the extracts obtained from different mushroom species to have different health effects. In recent years many clinical trials have been initiated to investigate the relevance of beta glucan supplementation. Traditional healers swear by the healing powers of functional mushrooms. Modern medicine is attempting to verify and specify the use cases where these fungi can have maximum impact. 

A major challenge lies in obtaining large quantities of pure beta glucan molecules. Only about 20% of the beta glucans are soluble without significant heating. Beta glucans consumed in the form of whole mushrooms are harder to digest. Extraction is a method of enriching beta glucans and increasing their bioavailability. 

Many mushroom extracts represent their concentration as ratios or in terms of polysaccharide content. These are misleading terms and do not indicate the effectiveness of the extracts. We always recommend that one checks specifically for beta glucan content when inspecting an extract for potential health benefits. Always remember that polysaccharides do not imply beta glucans.

Modern extraction methods aim to maximize and standardize beta glucan content per dose. In our next blog we will explore the latest extraction technologies and how they can help improve beta glucan yields.

References

Š. Karácsonyi and Ľ. Kuniak. 1994. “Polysaccharides of Pleurotus ostreatus: Isolation and structure of pleuran, an alkali-insoluble β-d-glucan”. DOI: 10.1016/0144-8617(94)90019-1

Milos Jesenak et. al. 2015. “β-Glucan-based cream (containing pleuran isolated from pleurotus ostreatus) in supportive treatment of mild-to-moderate atopic dermatitis”. DOI: 10.3109/09546634.2015.1117565

Ingrid Urbancikova et. al. 2020. “Efficacy of Pleuran (β-Glucan from Pleurotus ostreatus) in the Management of Herpes Simplex Virus Type 1 Infection”. DOI: 10.1155/2020/8562309

Juraj Majtan. 2013. “Pleuran (β- Glucan from Pleurotus ostreatus): An Effective Nutritional Supplement against Upper Respiratory Tract Infections?”. DOI” 10.1159/000341967

Yangyang Zhang et. al. 2011. “Advances in lentinan: Isolation, structure, chain conformation and bioactivities”. DOI: 10.1016/j.foodhyd.2010.02.001

Takuma Sasaki and Nobuo Takasuka. “Further study of the structure of lentinan, an anti-tumor polysaccharide from Lentinus edodes”. DOI: 10.1016/S0008-6215(00)83552-1

Yiran Zhang et. al. 2018. “Lentinan as an immunotherapeutic for treating lung cancer: a review of 12 years clinical studies in China”. DOI: 10.1007/s00432-018-2718-1

Hiroshi Hikino et. al. 1989. “Mechanisms of Hypoglycemic Activity of Ganoderan B: A Glycan of Ganoderma lucidum Fruit Bodies”. DOI: 10.1055/s-2006-962057

Hiroshi Hikino et. al. 1985. “Isolation and Hypoglycemic Activity of Ganoderans A and B, Glycans of Ganoderma lucidum Fruit Bodies”. DOI: 10.1055/s-2007-969507

Masashi Tomoda et. al. 1986. “Glycan structures of ganoderans b and c, hypoglycemic glycans of ganoderma lucidum fruit bodies”. DOI: 10.1016/S0031-9422(00)83748-6

Xue-Song Yang et. al. 2009. “Effects of Ganoderan on the dysbacteria in intestinal tract of mice”. DOI: 10.1096/fasebj.23.1_supplement.980.2

Weifeng Wang et. al. 2019. “Ganoderan (GDN) Regulates The Growth, Motility And Apoptosis Of Non-Small Cell Lung Cancer Cells Through ERK Signaling Pathway In Vitro And In Vivo”. DOI: 10.2147/OTT.S221161

Yifeng Zhang et. al. 2013. “Schizophyllan: A review on its structure, properties, bioactivities and recent developments”. DOI: 10.1016/j.bcdf.2013.01.002

Just like plant cells and unlike animal cells, fungi possess a cell wall. The cell wall is nothing but a protective layer within which all of the cellular components are housed. It acts as a protective shield that allows a fungal cell to carry out its functions without threat of destruction from external stresses. While it is easy to think of the cell wall as just a hard shell like that of a tortoise or turtle, this could not be farther from the truth. The cell wall is a highly dynamic and responsive organ of the fungal cell. This dynamic organ is both a target of antifungal drugs and enabler of immune evasion. It also contributes to a majority of the functional benefits we obtained by the consumption of mushroom and mushroom based products. It is constantly changing in response to stimuli and is key to the survival of a fungus. In this blog we will get an idea of the composition of the cell wall.

 

What is the cell wall

 

The cell wall of a fungus can be distinguished into two layers: an inner rigid layer and an outer fluid layer. The inner layer forms the structural component of the cell wall. It determines the shape and size of the fungal cell and it prevents the cell from bursting by taking in too much water.

 

The cell wall is located outside the cell membrane. Ninety percent of the cell wall is composed of polysaccharides. Polysaccharides are long chains of carbohydrate molecules known as monosaccharides bonded together to form a polymer. The most common monosaccharide molecule found in fungal polysaccharides is glucose. Galactose and mannose are also found in lesser proportions. Individual monosaccharides are connected to each other through glycosidic linkages. Polysaccharides are named based on their monosaccharide composition and the nature of the glycosidic bonds between the monosaccharides. 

 

The remaining ten percent of the cell wall may be made up of proteins, lipids and other minor biomolecules. The three major polysaccharide components of the fungal cell wall are: mannoproteins, beta glucans and chitin. Let’s have a look at these three in a bit more detail.

Cell Wall Components

 

Fig: Cartoon representation of a Candida albicans (yeast) cell wall (Source)

 

Layer 1: Mannoproteins

Mannoproteins form the outermost layer of the cell wall. This layer consists of polysaccharide chains composed mainly of mannose. A variety of proteins can be found attached to these mannan chains and therefore they are called mannoproteins. This layer is more fluid and porous than the other layers. As the outermost layer, it serves the important function of interacting with the surroundings of the fungal cell. The composition of this layer is highly variable across different fungal species and therefore the least is known about this layer.

Layer 2: Beta Glucans

Fig: Representative structure of fungal beta glucans consisting of long 𝛃 (1-3) chains with short branches of 𝛃 (1-6) linked D-Glycopyranosyl units (Source)

The next layer of the cell wall is made up of the famous beta glucans. These form the core structure of the cell wall and form the link between the inner rigid layer and the outer flexible layer. They are composed of long chains of glucose molecules linked to each other via 𝛃 (1→3) glycosidic bonds. These long chains contain short branches also composed of glucose which are linked through 𝛃 (1→6) glycosidic bonds. This results in the formation of a mesh or net-like structure in which other components such as proteins can be embedded.

Layer 3: Chitin

Fig: Representative structure of a chitin molecule consisting of 𝛃 (1-4) linked N-acetyl-Glucosamine (Source)

 

The innermost layer of the cell wall is composed of chitin. This is the most rigid part of the cell wall. Chitin is the most abundant molecule in the cell wall. Chitin and beta glucans are bound together and form the structural part of the cell wall. The chitin molecules form short chains organised into structures known as fibrils. 

These are helical structures that wind around each other (much like a rope). The fibrils of chitin assemble to form tightly packed sheets.  Due to this packing it acts as the protective layer of the cell wall, shielding the cell membrane from the harsh outer environment. The beta glucans are found attached to these chitin sheets.

Chitin is the oldest component of the fungal cell wall, surviving through evolution to become the most abundant molecule in the cell wall. Many fungi develop thick chitin layers when the cells are exposed to stress. 

The ratios of the different cell wall components are highly variable based on the fungal species. This species to species variation is sometimes used in their identification. 

Functions of the cell wall

The cell wall performs many functions in the fungal cell. Its most obvious function is protection. It also acts as the main structural backbone of the fungal cell, determines the shape and size of the cell and helps some fungi evade immune responses.

By forming a multilayered barrier around the fungal cell, the cell wall helps the cell withstand stressful conditions. The cell walls respond to different stresses by altering their composition. For instance, research shows that Aspergillus niger cells can increase the chitin composition of their cell walls by 2.5 times when they are starved. This results in the formation of a thick protective layer that allows the fungus to survive till it encounters a new nutrient source.

As fungi often live in highly variable environments, the composition of molecules such as salts and minerals are constantly changing in their surroundings. The change in salt concentrations lead to the formation of diffusion gradients which result in changing water levels within the cell. In conditions of low salt, the fungal cell swells up due to increased water content. As the cell swells, the cell membrane pushes against the cell wall and exerts pressure on it. These internal pressures can reach as high as 10 MPa in certain fungi. This is 20 times the normal atmospheric pressure! Without the cell walls, the cells would burst.

Fungi which form mycelial threads have elongated cells attached end to end and form highways for the transport of nutrients and water from one end to another. This movement of nutrients is essential to the maintenance of soil ecology. 

The fungal cell wall is of immense importance to human health. As it is the interface between the fungus and the external world, it acts as a beacon to our immune system indicating an infection. Most of the major components of the fungal cell wall can be detected by proteins on human immune cells. Upon detection, the immune cells release signaling molecules that inform the immune system of a fungal invasion and trigger an immune response. 

To take advantage of this directed immune response, many pathogenic fungi have evolved mechanisms to avoid their detection by altering their cell wall chemistry. These chemical modifications prevent the detection of the fungal cells by our immune cells and allow the fungi to go unnoticed. Other fungi can secrete molecules that act as decoys and prevent the recognition of the fungal cell walls by masking the proteins of the immune system.

 

Fig: Transmission Electron Microscope images of cell walls from three different fungi showing the variability in cell wall structure (Source)

 

Synthesis of the cell wall

The formation of the fungal cell wall is a complicated process with multiple steps along the way. Most of the molecules that form the cell wall are assembled within the cell in by the activity of enzymes. These enzymes string together molecules one by one to form the chains that make up the polysaccharides of the cell wall. 

Both chitin and glucans are extruded through the plasma membrane of the fungal cell. The basic ingredients for the formation of these molecules are nucleotide diphosphates. In the case of chitin and beta glucans, uridine diphosphate-sugars (UDP) are the carriers that bring together the monosaccharides which form the polysaccharide molecules that are incorporated into the cell wall.

Chitin is synthesised by a family of enzymes known as chitin synthases. These enzymes act on UDP-N-acetylglucosamine. The type of chitin synthase and their relative proportions in the cell determine the structure of the chitin molecules that end up in the cell wall. Similarly, beta glucans are formed the the activity of enzymes belonging to the glucan synthase family. These enzymes string together UDP-glucose molecules.

These enzymes can be found coexisting with each other near the cell wall where they actively extrude these components. Once the polysaccharide molecules are extruded, local enzymes embedded in the cell wall chemically link them to form the complex, multi-layered structure that we observe. The constant change in the number and kinds of proteins in the cell wall affect their structure and help the fungi adapt to various environmental conditions.

Fig: Cartoon representation of fungal cell wall synthesis. Chitin and 𝛃 glucans are synthesised by chitin synthase and 𝛃-1,3-glucan synthase enzymes respectively. The enzymes are embedded in the plasma membrane and extrude polysaccharide molecules to form the fungal cell wall (Source)

 

Conclusion

Up until the 20th century, the cell wall was assumed to be a passive structural element of cells. However, recent research has highlighted the constantly changing and highly regulated nature of the cell wall. As this structure forms the interface between the external and internal worlds of the cell, it needs to constantly adapt and mediate the survival of the cell. These structures have evolved to be highly complex and can vary immensely between species. However their two core components, chitin and beta glucans, have stood the test of time and are found in all fungi. As such they are responsible for many of the functional benefits observed when we consume fungi. In the next blog we will take a more detailed look at beta glucans and how they help our immune system.

 

References

Jean-Paul Latgé. 2007 “The cell wall: a carbohydrate armour for the fungal cell” DOI: 10.1111/j.1365-2958.2007.05872.x

E.P. Feofilova. 2010 “The Fungal Cell Wall: Modern Concepts of Its Composition and Biological Function” Microbiology. DOI: 10.1134/S0026261710060019

K. Vega and M. Kalkum. 2012 “Chitin, Chitinase Responses, and Invasive Fungal Infections” International Journal of Microbiology. DOI: 10.1155/2012/920459

Neil A.R. Gow et. al. 2017 “The Fungal Cell Wall: Structure, Biosynthesis, and Function” Microbiology Spectrum. DOI: 10.1128/microbiolspec.FUNK-0035-2016

Ruiz-Herrera and Ortiz-Castellano. 2019 “Cell wall glucans of fungi. A review” The Cell Surface DOI: 10.1016/j.tcsw.2019.100022

De-Oliva Neto et. al. 2015 “Yeasts as Potential Source for Prebiotic β-Glucan: Role in Human Nutrition and Health” DOI: 10.5772/63647

In Part 1 of this 3 Part Series we discussed the ways in which scientific studies have shown Pleurotus tuber-regium to aid and help with cancer.

Part 2 will take a deep dive into one of the most prolific diseases of our current generation. 

Diabetes is a disorder that renders the body incapable of dealing with its own glucose load. While there are many ways in which a person can reach the point of being diabetic, one thing is common among them all – diabetes is not fun! Diabetes leads to impaired wound healing, improper weight maintenance, the need to supplement with medication on a daily basis, and an overall decreased quality of life. India is currently the diabetes capital of the world with over 70 million diabetics in the country as of 2019. An increase in packaged and processed foods along with sedentary lifestyles and carbohydrate-laden Indian diets have been heavy contributors to this rise. 

Part 2: Diabetes

Pleurotus tuber-regium for diabetes

Many laboratories around the world have been looking towards mushrooms as a potential tool to reduce the rates of diabetes occurrence.
Mushrooms contain a wide variety of bioactive molecules that can contribute towards a healthier physiology

In 2012 researchers from Taipei investigated the effects of Pleurotus tuber-regium polysaccharide extracts on rats that were made diabetic. The rats were injected with a drug called streptozotocin. This compound is particularly toxic to pancreatic beta cells which are responsible for the production and secretion of insulin in a normal body. The loss of insulin-producing cells pushed the mice toward diabetes. The mice were also fed a high-fat diet which further enhanced their diabetes. 

Polysaccharides were extracted from P. tuber-regium with hot water and precipitated with ethanol. The purified polysaccharides were administered to the diabetic rats every day for 8 weeks and The rats were monitored for weight gain and other common clinical signs of diabetes such as oral glucose tolerance, Hba1c levels, and serum insulin. To their surprise, the polysaccharide-treated rats gained less weight on high-fat diets when compared to untreated mice. Furthermore, the polysaccharide-treated rats also had higher insulin levels, lower Hba1c, and better glucose tolerance. The polysaccharide-treated rats it seemed were less diabetic.

Pleurotus tuber-regium versus fat

With some positive indications, the investigators delved deeper into the body weight mystery. They measured common health markers such as serum triglycerides and cholesterol levels. These circulating fats are bad for cardiovascular health when they stay abnormally high for many years together. Surprisingly the researchers found that the polysaccharide treatments also resulted in lower cholesterol and triglyceride levels indicating that the polysaccharides were possibly affecting fat distribution in the body as well. This could explain the lower weight gains observed in the polysaccharide-treated rats.

In an extension to the above study, the scientific team looked at how the liver was affected by the polysaccharide treatment. The liver is at the center of many metabolic pathways, including cholesterol and triglyceride metabolism. Since the polysaccharides were affecting the serum cholesterol and triglycerides, the liver was an obvious place to take a deeper look. They discovered that a protein named PPAR-⍺ was more prevalent in the livers of the polysaccharide-treated rats. PPAR-⍺ increases the expression of genes responsible for fat metabolism. The polysaccharides were able to stimulate the metabolism of fats in the liver thus resulting in lower triglyceride levels. 

Combo, that works!

The high fiber content in P. tuber-regium extracts and whole mushrooms should not be ignored when considering these effects. Soluble dietary fiber is known to reduce fat and cholesterol absorption in the intestine. The lower absorption of fats combined with the increased metabolic activity of the liver together could have reduced the metabolic burden on the diabetic rats. The extracts are also rich in flavonoids which have strong antioxidant properties. These antioxidants can reduce damage to cells and in particular may have aided in the survival of the pancreatic beta cells. 

So what does this mean?

So how is this information really relevant to us?

There is no evidence that states that the use of such extracts in diabetic patients will have similar effects. However consuming foods rich in antioxidants and fibers have been shown to aid in healthy aging. Diabetes in most cases is a result of metabolism going bad. When we are young and relatively active, our body is more capable of dealing with the stresses of daily life. As time progresses, the accumulating stress overcomes the natural defenses the body has and pushes it towards a disease state. By aiding the body’s fight against the disease state, health span increases even if lifespan does not. In fact, it has been shown that chronic high-fat diets lead to insulin resistance and eventually diabetes. Tackling the issue from the onset can help offset this pathway and may even prevent it. 

Low serum triglycerides and cholesterol are important indicators of good cardiovascular health. It is most often cholesterol and triglycerides that end up being deposited in the small arterioles and venules of our bodies. The deposits eventually block these capillaries and can lead to what is commonly known as heart attacks, thromboses, or strokes depending on where they occur. By reducing fat absorption, high-fiber diets help support cardiovascular health. By preventing the build-up of sludge, flavonoids keep our capillaries healthy. 

Biochemical analysis of P tuber regium fruiting bodies showed that they are rich in proteins, fibers, and carbohydrates and low in fats. Carbohydrates in this case do not equate to glucose since the carbohydrates here refer more to complex carbohydrates and will not contribute to the glucose load as much as starch prevalent in many other vegetables. These mushrooms also contain all of the essential amino acids making them a complete source of protein. Obese and diabetic rats fed with dried and powdered P. tuber-regium had similar serum lipid profiles to rats treated with the antidiabetic drug Glibenclamide. 

1. tuber-regium is a unique mushroom that has been used by traditional communities for hundreds of years to help treat various ailments. Are they truly medicinal, we can’t say for sure. They sure are delicious though!

Stay tuned for the final part of this series!

References

Ikewuchi, Catherine Chidinma et. al. 2017. “Restoration of plasma markers of liver and kidney functions/integrity in alloxan-induced diabetic rabbits by aqueous extract of Pleurotus tuberregium sclerotia”. Biomedicine and Pharmacotherapy.  DOI:10.1016/j.biopha.2017.09.075

Huang, Hui-Yu et. al. 2012. “Pleurotus tuber-regium Polysaccharides Attenuate Hyperglycemia and Oxidative Stress in Experimental Diabetic Rats”. Evidence-Based Complementary and Alternative Medicine. DOI:10.1155/2012/856381

Huang, Hui-Yu et. al. 2014 “Effect of Pleurotus tuber-regium Polysaccharides Supplementation on the Progression of Diabetes Complications in Obese-Diabetic Rats”. Chinese Journal of Physiology. DOI:10.4077/CJP.2014.BAC245

Onuekwuzu, Ifeanacho et. al. M 2019. “Anti-Diabetic Effect of a Flavonoid and Sitosterol – Rich Aqueous Extract of Pleurotus tuberregium Sclerotia in Alloxan-Induced Diabetic Rabbits”. Endocrine, Metabolic and Immune Disorders – Drug Targets. DOI:10.2174/1871530319666190206213843

Lin, Shaoling et. al. 2021. “Research on a Specialty Mushroom (Pleurotus tuber-regium) as a Functional Food: Chemical Composition and Biological Activities”. Journal of Agricultural and Food Chemistry. DOI:10.1021/acs.jafc.0c03502

Adeyi, Akindele Oluwatosin et. al. 2021. “Pleurotus tuber-regium inclusion in diet ameliorates dyslipidaemia in obese-type 2 diabetic rats”. Clinical Phytoscience. DOI:10.1186/s40816-021-00321-0

 Tyagi, Sandeep et. al.  2011. “The peroxisome proliferator-activated receptor: A family of nuclear receptors role in various diseases”. Journal of Advanced Pharmaceutical Technology and Research. DOI:10.4103/2231-4040.90879

Lattimer, James M. and Haub, Mark D. 2010. “Effects of Dietary Fiber and Its Components on Metabolic Health”. Nutrients. DOI:10.3390/nu2121266

When we were starting our journey, we were completely new to the mushroom space and hardly knew anything about cultivating them. We took up mushroom cultivation because it seemed like something that could meet our goals- sustainable, profitable and scalable. If you want to know more about us and why we do what we do, please check out our About us page.

Our journey into the Indian mushroom cultivation space was far from smooth and had a lot of ups and downs. The important thing is that we made it this far and have figured out a lot of interesting things on the way which would have saved us a lot of time, effort and money if we knew it earlier.

This blog post is our way of passing on some important bits and pieces of information that we have acquired in our journey so that beginners don’t have to go through all the trouble we did.

 

1.     Choose your mushroom well

Each mushroom comes with its own set of advantages and disadvantages, some are easy to grow and grow really fast, others can fetch you a lot more money but they might be quite challenging and expensive to cultivate. You have to ask yourself a few questions which will help you find the mushrooms best suited to your requirements. Here are some questions that you can think about: 

• How much time and money am I willing to invest in this business? 
• How long can I wait before each harvest? 
• Which mushrooms are best suited for the climate around me? 
• Do I want to grow mushrooms seasonally or throughout the year? 
• Can I find the raw materials required to grow the mushrooms around me in an economical way?  

Mushrooms are not just what you see in the supermarket, they are also ingredients in various different industries such as food and beverage industry, nutraceuticals and functional foods, personal care, retail etc and are consumed in different forms – whole dry mushrooms, dry mushroom powder, fresh mushrooms, mushroom extract etc. When choosing your mushroom, you also need an understanding of where all it can be used and in what all forms.

Most trainers and courses will recommend Oyster mushrooms as a good mushroom to start with. They are not wrong but they aren’t completely right either. Yes, Oyster mushrooms are really easy to grow and they are quite forgiving of our mistakes. If you dig deeper, you will find that they have a really short shelf-life of 2-3 days from harvest. To make matters worse you will also realize that there is hardly any demand for them in the Hotels/Restaurants/Café sector because of their unpopularity among Indians. All the above factors make oyster mushrooms a not-so-good candidate for large-scale farms. You might be tempted to grow Button mushrooms because the demand for them is high but you cannot cultivate them without a climate-controlled setup (unless your local weather conditions are suitable) since they need temperatures between 16-19 degrees Celsius for it to fruit. So, please consider all angles before you choose which mushrooms to grow. Don’t just follow trends, take time to understand what has demand in your area and what can be grown easily.

2.     Understand the market

Once you have chosen which mushroom to cultivate, you have to study the local market for the same in-depth. You will have to find out before-hand which are the industries and channels that are currently using your mushroom and at what volumes and at what price. Try and talk to others in the same business if possible, to understand challenges that they might have faced. Make sure that you factor in things like ease of transportation and cost of packaging while you choose which channels to focus on. Understanding the market also means understanding the pain-points of the existing market so that you are better equipped at dealing with them. This step is very critical to ensure you succeed because without understanding the market you will not know how to position yourself or how to market your finished product.

3.     Develop your own market

After you have done an in-depth study of the market, you have all your facts and figures ready. Some questions to ponder at this stage are: 

• Which segments of the market can you realistically satisfy?
• What can you do to make your product stand out from the local competition?
• Some retailers require you to give them a fixed quantity on a daily basis, can your production meet those demands? 
• What resources do you need to satisfy the capacity you have settled on? 

Remember, you can always start small and scale up. For example: to get a feel of the fresh mushroom market start by selling to close friends and family or to your neighbors, to create your own loyal client base. You could even drop off samples at restaurants nearby and ask the chefs to give your mushrooms a try. Figure out what matters most to them (The freshness? Value for money? Packaging? Aesthetics?) and in the process learn how to best satisfy your customer. The easiest strategy would be to work with a few customers in such a way that you have forward orders for your harvest. You are ensuring that you have a ready market for your produce so that you can put all your energy and attention into cultivating high quality mushrooms in a way that your customers love them.

4.     Get the right training

This step is extremely crucial and the step which we here at Nuvedo had a hard time with. When we were starting out, we could hardly find any courses (both online and offline) which seemed legitimate. The courses were either too basic and lacked scientific explanation behind the processes involved or promised far too much to seem realistic – like lifetime support on cultivation and timely updates on technical advancements in cultivation for a fee of INR 750? I don’t think so! 

In our experience, we found that a lot of trainers were ex-mushroom cultivators themselves but turned away from cultivation and towards training because it was more lucrative. I don’t think that this is a good sign. If you’re getting trained make sure that it is under someone who either has a successful mushroom cultivation up and running or from a reputed consultant with a proven track record. We here at Nuvedo offer multiple avenues for different experience levels and requirements- starting from our DIY Mushroom growing kit for beginners to advance hands-on courses for specific mushrooms. Check out our Workshops section to stay up to date with our latest in-person workshops..

5.     Have a trusted vendor for spawn and raw materials

Once you have had some basic training and experience growing your first batch of mushrooms, you need to figure out how to get quality inputs for cultivation. The most critical input in ensuring you get a profitable yield is having good and reliable spawn. Without good spawn, you are bound to fail. You have two options: either 1) make your own spawn, which is time consuming and involves considerable effort or 2) buy it from a vendor, which might be a bit risky. When looking for vendors, make sure you find vendors who give you at least generation 2 spawn and have a hygienic and standardized setup with documented results. In India, getting high quality spawn can be a challenge because of lack of regulations or standards in the industry. If you want to ensure that what you’re paying for is of the quality that they promise, you can go through our in-depth blog on spawn. We here at Nuvedo have our own super spawn- NuvoSpawn, which is manufactured in our lab using the latest technology and best quality ingredients. We have documented the conditions in which our spawn performs best, so that you can get best yields each and every time. To know more about what spawn is and how it is used please visit our Spawn FAQ.

Apart from spawn, ensure that you have a steady source of substrate material that is available locally and at a cost that makes sense for your profitability goals. Different substrates can have varying effects on yield and quality of the mushrooms that you grow. If you want to know more about the effect that different substrates and techniques can have on your yield and quality, do check out our DIY Mushroom Growing Kit. As part of the kit, we provide an information pamphlet with various different substrate recipes along with a table to track your progress. The pamphlet contains basic definitions and even some formulas to calculate biological efficiency, hydration etc. 

6.     Prepare yourself for failure

Making one grow bag of Oyster mushrooms during training doesn’t prepare you well enough for the challenges that you will face while handling a bigger operation. Most people fail in the beginning when they try cultivating mushrooms at a commercial scale because there are too many variables involved and it is extremely hard to standardize the whole process. So, make sure that you are mentally prepared to accept failure. Your strategy should be- fail small and fail fast, so that you learn from your mistakes quickly and they don’t cost you so much money. You have to ensure that you document every single step so that you have evidence of what worked and what did not. This will allow you to finetune your entire process and develop standard operating procedures for each step. Standardizing your process and steps involved is critical to ensure that you can reproduce your results every time.

7.     Start Small and scale up

Your trainers or consultants might tempt you to go for a 100 Ton setup and you might be tempted to do so after your successful oyster grow but always start small. Start at a capacity where you are confident that you have a ready market for whatever it is that you produce. Once you have mastered the art of cultivating at a small or intermediate capacity then you can confidently invest money in the right places to increase your capacity and scale up production. Remember, selling mushrooms can be harder than growing them, so ensure that you have a ready market before you decide to scale up production. Starting small will allow you to make changes to your layout or process flow without incurring large losses. 

8.     Build a larger ecosystem of cultivators in your area

Like any other business, mushroom cultivation can fall prey to a lot of issues which can affect your turnover. Drastic changes in weather, contaminated spawn, equipment failure, labor shortage etc. can negatively impact your business. This is why it is important for you to ensure that you have a network of mushroom cultivators in your locality who can help you in case of emergencies like equipment failure or loss of cultures due to contamination etc. We at Nuvedo take pride in saying that we work closely with all our community members in ensuring that they succeed. Nuvedo gives its network of cultivators access to documented cultivation techniques, new advancements in cultivation technologies and also a list of our trusted vendors across the mushroom cultivation ecosystem. Nuvedo also has a group of highly trained and experienced professionals who help our customers troubleshoot and get the best possible output from their cultivation unit. We cannot thank our community members enough for all the support they’ve provided us on our journey so far. 

9.     Think about other streams of revenue

Apart from just cultivating and selling fresh mushrooms it helps to have other streams of indirect revenue coming in. For example, dehydrating and maintaining an inventory of dried mushrooms, making value added products from your mushroom such as pickles, soup powders, cookies etc. These additional sources of income ensure that you can protect yourself from market disruptions and other influences beyond your control. In the hypothetical case that the price or demand for fresh mushrooms drops in your locality, you now have the option of drying them and using them in your value-added products. With India still reeling in the aftermath of the pandemic, it is critical now more than ever to diversify your business into multiple streams of revenue so that you can keep your business safe from external challenges.

10.   Keep learning and share your knowledge

Mushroom cultivation is a fairly new space and there are lots of technological advancements being discovered on a daily basis. It is important to keep updating our knowledge on the latest developments in the field of fungiculture so that we can continuously improve the quality and output of our cultivation unit. It is equally important to share whatever knowledge we have with others in the mushroom ecosystem so that the whole community can develop and better serve the market. In today’s day and age the most successful companies keep much of their information open source as they know the importance that sharing the right knowledge can have on the larger business ecosystem. Investing time and resources in keeping yourself up to date and in innovation has a very large payoff in the long run. We here at Nuvedo, live by the philosophy “A thousand candles can be lit from the flame of one candle, and the life of the candle will not be shortened” . So go on and spread the spores of knowledge far and wide so that at least some of them can fruit when the conditions are right. 

 

Pleurotus tuber regium. The King Tuber Oyster Mushroom. This warm-weather mushroom has long been revered for its medicinal properties in parts of Western Africa, China and North East India. As the only sclerotium-forming member of the Pleurotus genus, it is unique in its ability to store nutrients for a rainy day. Here is a 3 part series taking a look at 3 diseases that this wonderful mushroom can protect you from.

 

In Part 1, we will refrain from fixating on the evolutionary wonders of the sclerotia but instead, look to them as sources of medicinal compounds. In particular, we will investigate some existing evidence relating the sclerotial extracts to antitumor or anticancer properties.

Part 1: Cancer

Beta-glucans as active molecules

Our journey begins at the Chinese University of Hong Kong where Peter Cheung and colleagues made use of hot alkaline water to extract polysaccharides from the sclerotia of P. tuber-regium. Polysaccharides are long chains of sugar molecules such as glucose that are connected to each other through chemical bonds. These polysaccharides are important structural components of every fungal cell. In their study, the researchers chose to focus primarily on the long and branched polysaccharide molecules known as beta-glucans.

The researchers used two commonly used cancer cell lines to test the effects of their beta-glucan extracts. HL-60 cells are derived from a patient suffering from acute myeloid leukemia (AML), a form of blood and bone marrow cancer that is also the most common form of such cancer in adults. When they treated the cells with their beta-glucan extracts, they noticed that the outer membranes of the cells became more porous. A blue dye called Trypan blue which is normally kept out of the cancer cells by the cell membrane was able to enter the cells and stain them blue. This indicated that the extracts were able to disrupt the integrity of the cell membrane of the leukemia cells. 

To verify whether the extracts could work on other forms of cancer, the researchers used another cancer cell line called HepG2 cells. HepG2 cells are derived from hepatocellular carcinoma (a form of liver cancer) and are commonly used to screen drugs for toxicity and study liver cancer. When these cells were treated with the P. tuber regium beta glucan extracts their metabolic activity decreased. A decrease in metabolic activity indicates stressed, dying, or non-functional cells. This indicated that the P. tuber regium beta-glucan extracts had a negative effect on the HepG2 cells as well.

At this point, an obvious question that the researchers had to answer was whether the adverse effects seen by the extracts were only on cancer cells. The extracts could have similarly deleterious effects on normal and healthy cells too, which would make them hazardous for consumption. To check if this was true, they tested the extracts on kidney cells obtained from an African green monkey (Vero cells) and found that the effects that they had observed on the cancer cells could not be seen here. This was an indication that the extracts were more harmful to cancer cells than to normal cells.

The importance of solubility

Now that they had some evidence that the beta-glucan extracts from P. tuber regium had some anti-cancer effects, the scientists wanted to improve the solubility of the extracts to make them more easily consumed. To do this, they modified the beta-glucan molecules by adding water-loving or hydrophilic chemical groups to them through a process known as carboxymethylation. This made the molecules easily soluble in water and therefore more bioavailable. They then added different doses of this modified beta-glucans to a human breast cancer cell line (MCF 7 cells). They observed a dose-dependent decrease in the metabolic activity of these cells. By monitoring the change in the number of DNA molecules in the treated and untreated cells they showed that the treated cells were multiplying much slower than the untreated ones. Further investigation showed that the treated cells were more prone to death as well. This highlighted the anti-cancer abilities of these extracts against breast cancer cells.

Size matters

The evidence provided by the studies so far sheds some light on the ability of P tuber regium sclerotial extracts to prevent or reduce the growth of cancer cells. However, a cell in a dish in the lab does not replicate the dynamic environment within the body. Another group of scientists from China’s Wuhan University decided to dissect this very aspect. They chose to work with Sarcoma 180 cells, a highly malignant mouse cancer cell line widely used in the field of cancer biology. They extracted water-soluble compounds from the sclerotia of P. tuber regium and further separated the molecules based on their sizes and biochemical properties. Once again the beta-glucans proved to be the molecules of interest. Moreover, the size of the beta-glucans had an impact on their effectiveness. Longer beta-glucan chains were more active when compared to the shorter ones. When it came to beta-glucans and their anti-cancer activities, size did matter it seemed.

In vivo effects

To replicate a more likely pre-clinical scenario, the Sarcoma 180 cells were transplanted under the skin of mice and allowed to form an active tumour. These mice were then injected with either 5-fluorouracil (an anticancer drug) or the beta-glucan extracts from P. tuber regium every day for eight days. On the ninth day, the tumours were dissected and weighed. Two of the extracts tested outperformed 5-fluorouracil and showed a greater reduction in tumour weight. This indicated that the extracts were not only able to prevent cancer cell growth in a dish, but also do so when injected into live animals. 

 

Taking a look at the evidence so far points to the ability of the polysaccharide extracts from Pleurotus tuber regium to inhibit the growth of cancer or tumor cells. However, it is important to keep in mind that all of these studies have been performed under highly controlled laboratory settings using well-established, immortal cell lines and do not necessarily recapitulate the true behavior of the disease. The in vivo mouse study by Zhang et. al. does indicate that the injected molecules are able to find the tumor and affect its growth negatively. However, the mode of injection, the location of the tumor, and the dosing protocol did not perfectly reflect an actual clinical scenario in a human patient. No successful clinical trials have been conducted to date that conclusively shows the efficacy of such extracts in the treatment of cancers or tumors. Keeping this in mind, we must evaluate the evidence that continues to grow day by day. Can these mushrooms help in the treatment of cancer?

Only time can tell. But they sure are delicious!

Read Part 2 on Diabetes of the blog King Tuber Oyster Mushroom: 3 Diseases it can protect you from: (PART 2/3)

References

Cheung, Peter C. 2001. “Chemical structure and chain conformation of the water-insoluble glucan isolated from Pleurotus tuber-regium.” Biopolymers.

Zhang, L. 2001. “Evaluation of mushroom dietary fiber (nonstarch polysaccharides) from sclerotia of Pleurotus tuber-regium (Fries) singer as a potential antitumor agent.” J Agric Food Chem.

Zhang, Lina. 2008. “Characterization of polysaccharide-protein complexes by size-exclusion chromatography combined with three detectors.” Carbohydrates Research. 

Zhang, Lina. 2009. “Chemical modification and antitumor activities of two polysaccharide-protein complexes from Pleurotus tuber-regium.” Int J Bio Macromol.

Zhang, Mei. 2003. “Molecular mass and chain conformation of carboxymethylated derivatives of beta-glucan from sclerotia of Pleurotus tuber-regium.” Biopolymers.

Zhang, Mei. 2004. “Carboxymethylated β-glucans from mushroom sclerotium of Pleurotus tuber-regium as novel water-soluble anti-tumor agent.” Carbohydrate Polymers.

 

Before you start exploring this blog about mushroom spawn, It will be really helpful if you are familiar with the basics of what mushrooms are and how they are cultivated. If you’re new to mushroom cultivation, please check out these blogs to know more about how mushrooms grow and the jargon used in mushroom cultivation:

• Lifecycle of a Mushroom
• Glossary | All things Fungi

Mushroom spawn is very critical if not the most important input in the mushroom cultivation process. We try to clarify the most commonly asked questions about mushroom spawn in this blog, in order to equip you with the right knowledge. Here is a look at the different questions that we will be addressing:

• What is mushroom spawn?
• Why can’t we cultivate from spores?
• How is spawn made?
• What is the difference between grain spawn and sawdust spawn?
• What are the qualities of good spawn?
• How do we store spawn properly?
• How do we know if the spawn we have is good?

What is mushroom spawn?

Mushroom spawn is basically mycelium, the living fungal culture, grown onto a substrate. It is the most critical input in mushroom farming and is used by mushroom growers similar to how farmers and gardeners use seeds. Mushroom spawn, unlike seeds, is grown from selected genetics and cloned so that it is possible to consistently produce a particular cultivar (cultivated variety) of mushroom which exhibits desired traits. This is similar to how people grow fruit trees via grafting as opposed to planting their seeds. Grafting is done to make sure that the fruit tree consistently produces delicious fruits because of a particular set of genetics that are chosen. Spores (and seeds for that matter!) are a genetic lucky dip dependent on two individual sets of genetic material, whereas spawn is a single, unique genetic culture that can be indefinitely propagated from the same ‘mother’ culture. Our ‘mother’ cultures are kept in the laboratory on agar petri dishes and maintained at the optimal temperature.

 

Spawn bags being prepped under sterile conditions in the lab

 

 

Why can’t we cultivate mushrooms from spores?

In the wild, mushrooms produce tens of thousands of spores (some even billions!) and get scattered across the forest by wind, rain, insects and other agents. They are on a quest to find the most suitable growing conditions but sadly the vast majority of spores will never grow into a mushroom fruit-body. As a cultivator this is not a risk that you can take, you want to ensure that you get consistent, reliable and repeatable results every time.

Another factor to consider is that spores are not sterile and growing using spores directly might lead to an increase in contamination rates which will affect your productivity drastically.

At Nuvedo we select productive strains of edible and medicinal fungi to make spawn which have been proven to give consistent results in the Indian setting, so that our cultivators can maximize their success.

How is spawn made?

All spawn start out their journey on a petri-plate as a pure fungal culture of mycelium. Once the mycelium has fully colonized the surface of the agar, a tiny piece of the mycelium is transferred to boiled grain. This mycelium is then allow to grow on the surface of the boiled grain for 3-4 weeks until it colonizes all of the grain. This myceliated grain is what is called grain spawn.

 

Step 1

 

Step 2

Apart from different grains such as wheat, jowar (sorghum), millet, rice, etc. some spawn producers even use sawdust and wooden dowels as a substrate for making spawn. The substrate used to make spawn serves three functions-

• Act as a surface for the mycelium to grow and spread on
• Provide the mycelium with macro and micronutrients so that it stays alive and healthy till it is inoculated on the final substrate material
• Act as multiple points for the mycelium to grow from and colonize the final substrate material at a faster rate from different parts of the substrate

We produce all of our spawn at our state-of-the-art facility in Bengaluru. NuvoSpawn is produced on sorghum grain in sterile lab conditions. We start by taking mushroom cultures from our culture bank and then growing them out on sterilized grain in a controlled environment, using our own standardized process and media, to ensure that our customers get the best quality time and again!

To ensure consistent results we grow our own mushrooms at regular intervals and keep track of the cultivation parameters of each and every strain. We do not sell spawn of any mushroom that we ourselves have not grown. If you’re buying spawn for the first time, make it a point to ensure that your spawn vendor grows their own mushrooms to ensure the variety is still performing consistently.

 

Nonabsorbent cotton plugs being inserted into the neck of the spawn bag inside a Laminar Air Flow

 

Inoculation of sterilized sorghum with a myceliated agar wedge

 

 

Final packing of spawn bag after inoculation under aseptic conditions

 

What is the difference between sawdust spawn and grain spawn?

Grain contains a lot more nutrition as compared to sawdust. This can lead to contamination or increased chances of attack by pests if used to make outdoor beds or logs. When the cultivator wants to grow in an outdoor environment where there could be pests or where the chances of contamination are higher, sawdust spawn is a much better option. Using sawdust spawn for conventional cultivation can lead to lower yields and slower colonization as compared to grain spawn.

What are the qualities of good spawn?

The most critical parameters for good spawn from a cultivator’s point of view is:

• It should be free from contamination

  1. The spawn has to be made under aseptic conditions preferably under a Laminar Air Flow to ensure the best results
  2. All materials used must be of the highest quality. Using low-quality or broken grain can lead to increased chances of contamination post inoculation.
  3. Grain used has to be boiled to the right consistency to ensure that it doesn’t break or get squished post inoculation. This is really important in ensuring low contamination rates.
  4. Grains/sawdust needs to be sterilized in an autoclave at 121 degrees Celsius and 15 PSI pressure to make sure that no microbial life persists

• It should be fast colonizing

  1. Genetics that are old or not maintained well undergo “senescence” or deterioration, leading to slow growth and poor yields

• It should give good yields

  1. Strains are one of the most critical factors which determine yield so spawn manufacturers should use commercial cultures which give high yields
  2. If cultures are not maintained well, the fungus can lose its virility over time leading to poor yields

• It should be free from toxic chemicals, antibiotics, and pesticides

  1. Some spawn manufacturers have been seen using hazardous chemicals such as formaldehyde to fumigate their labs and some even add antibiotics such as gentamycin to their media to keep it free from microbial contamination
  2. Over time, exposure to these chemicals can cause detrimental health issues to the cultivator who handles these materials
  3. Fungi can bioaccumulate complex molecules and the resulting mushrooms may contain trace amounts of these chemicals which will eventually affect the health and wellbeing of the consumer.

• It should give consistent results every time

  1. Some genetics are prone to mutation more than others and this can lead to variation in cultivation parameters such as speed of colonization, physical characteristics of the mushroom itself, and even yields.

 

Qualities of Good Spawn

We take pride in saying that NuvoSpawn is not just another bag of spawn. It is a technically superior product guaranteed to maximize your success by giving you consistent results time and again. We document the optimal growing conditions of our cultures to ensure that our customers can make the best use of our product.

NuvoSpawn:

• Is enriched with first-quality grain which ensures vigorous colonization and healthy growth.
• Is completely dry, pure fungus without wet patches to eliminate chances of bacterial contamination.
• Has been proven to give higher yields due to greater bio-efficiency because of our genetics and unique media.
• Is manufactured in a sterile environment which leads to a healthier growth of fungus.
• Uses disinfected nonabsorbent cotton to reduce chances of contamination.

 

How do we store spawn properly?

Your mushroom spawn is alive!! Yes, it is a living, breathing organism. In order to keep it healthy, happy, and strong we need to make sure that it is stored properly. A question we keep hearing is “how long can I store my spawn?” How long you can store your spawn depends on 3 things mainly:

• Cultivated variety or cultivar
• Storage Temperature
• Storage Conditions

 

Factors Affecting Spawn Storage

 

Let’s take a look at the factors one at a time:

• Cultivated variety:

It has been observed that varieties in which the mycelia grow slowly tend to have the longest shelf-life. To put it simply, the slower the growth of the variety the longer you can store it.

There are some basic signs to look for to understand if your mycelia are undergoing senescence or biological aging. The following are signs of the aging process of mycelia, in their order of appearance:

• The mycelia become more compacted
• The appearance of hard-looking crusts or lumps
• Formation of foul-smelling, colored liquid
• Self-digestion or autolysis of mycelium and degradation of mushroom spawn

 

4 Stages of Spawn Ageing

 

The mycelium is perfectly healthy and usable in stages 1 & 2 through the spawn might not feel as crumbly as it does when it is fresh. Self-digestion or autolysis starts happening at the end of stage 3, hence it is strongly recommended that you use your mushroom spawn before it happens. The mycelium has reached the end of its life in stage 4 and therefore the spawn should be discarded at this stage.

 

• Storage Temperature:

The ideal temperature for the storage of spawn is 0 to 4 degrees Celsius. At this temperature, spawn can be stored for anywhere from 2 months to 4 months. However, there are a few exceptions to this, for example, Pink Oyster mushroom spawn or Milky mushroom spawn tend to degrade if refrigerated since they are both tropical varieties.

 

• Storage Conditions:

If you have ordered boxes of NuvoSpawn, make sure that you place the boxes on shelves or stack them in an alternative manner like bricks, always making sure that you leave around 10 cm space between the boxes for airflow. If spawn for mushrooms like oysters has to be stored for extended periods, then take out each bag from the box and put them separately on the shelves inside the refrigeration unit. The refrigeration unit will have to be opened on a daily basis to ensure there is enough circulation of fresh air for spawn survival.

We strongly recommend you not to order or store your spawn months before the actual date of use. Whatever money you may save on shipping will be compensated by an increased yield and lower chances of contamination losses using fresh spawn.

At Nuvedo we do not keep any spawn that has aged more than 3 weeks to ensure that our customers get the best results, so it becomes really difficult to entertain last-minute requests as we are almost always booked out. The best-case scenario for us would be if you let us know 14-21 days before you need your spawn so that we can ensure the availability of fresh spawn. We will ship it out to you exactly in time for your inoculation!

 

How do we know if the spawn we have is good?

So, you’re waiting on your first batch of NuvoSpawn from Nuvedo or have a batch of mushroom spawn that you’ve been storing for a while now?
Without testing, you might have to inoculate your substrate and then wait a few weeks to come to the realization that the spawn you used was too old or not strong enough. To save yourself all that trouble you now want a quick and easy method to see if you can proceed with inoculation without worrying if the spawn you used was good enough. Well, you’ve come to the right place, all you need to do is follow the instructions mentioned below!

There are 2 ways of doing this, the hard way (which gives you more reliable results) or the easy way (which is cheaper and requires a lot less effort)

Let’s start with the easy way:

1. Take a sample of a few grams of spawn from each bag you wish to test.
2. Take a clean plastic container and put a small pile of wet paper (tissue paper, toilet paper, cardboard) on it.
3. Place the spawn on top of the paper.
4. Place the container in a clean, cool place away from direct sunlight.

The mycelium should be growing visibly on the paper in less than a week. This method is not foolproof and can give you false results so we would recommend you to follow the technique mentioned below.

The hard but reliable way:

1. Take a petri dish that has been prepared with PDA or MEA mixture.
2. Open the dish under sterile conditions, preferably under a laminar airflow, to avoid contamination. (Contamination can give your false results)
3. Using a sterile tool, such as a spoon sterilized under a naked flame, place a few kernels of mushroom spawn around the petri dish.
4. Under the laminar air flow, roll the kernels around under the petri dish. Close and label the petri dish.
5. Let the petri dish mature for 5 days to a week at a temperature beneficial for mycelium. We recommend around 20 Degrees for Oyster mushroom spawn or Shiitake mushroom spawn.

In a week you should observe mycelium growth from the place where the kernels were rolled over the agar medium and from the kernels themselves

The result: The strength of the spawn is indicated by the amount of mycelium growth present after a week. If the growth is fast and intense, your spawn is still very active. Old spawn also has a capacity to colonize the substrate like fresh spawn but the rate of growth will be much slower. So, the same applies to the kernels on your petri dish/paper towel. If you don’t see any growth in 5 days or so then that means that your spawn is too old and needs to be discarded. It is not that old spawn will not be active, it grows so slowly that contaminants get the upper hand and might take over your substrate. So, better safe than sorry.

Now that you have checked your spawn quality, you can confidently proceed with the next steps of mushroom cultivation if your test results came out well or order a fresh batch of NuvoSpawn in case your spawn is too old.

This brings us to the end of this blog, if you have any further questions or need any clarifications about spawn, feel free to reach out to us. We are more than happy to answer your queries.

Before I get into the mushroom side of things, let’s take a look at 3 not-so-good statistics about the current state of affairs in India –

1. 2022 was the hottest summer in India in the last 10 years with temperatures in parts reaching 49 degrees Celsius.
2. According to the latest National Family Health Survey (NFHS-5), India witnessed a 62% increase in the number of overweight children from 2016 – 2020. This alarming statistic is an indication that if neglected, we might face an obesity crisis.
3. Despite the blanket ban on plastics nationally, India still generates over 26,000 Tonnes of plastic every day with 60% of this ending up in landfills.

So, to put it simply- global warming is showing its true colors, our population is getting more obese by the day and our lands are being choked by plastic waste.

The million-dollar question on your mind now must be – what does all this have to do with mushrooms?!

Well, let’s find out!

As humans, we are drawn to mushrooms because of the unusual physical characteristics of their fruitbodies which magically appear after the first rains in fields and woodlands. Mycelium, which might not be visible, but is passively present within the ground below, waiting for favorable conditions.

Pleurotus tuber-regium has a similar story for us. This beautiful ochre colored mushroom was first stumbled upon by a member of our Cultivation Team at Nuvedo,while on a trip through the tropical jungles of Goa. He was quick to grab his camera and send us a couple of photos of this unique looking mushroom. Little did we know that this mushroom would soon be the reason for a lot of research, experiments, discussions and sleepless nights in our journey to work with the most healing mushrooms out there.

Ethnomycological Significance

Pleurotus tuber regium or King Tuber Oyster mushroom as it is commonly called is a relatively new mushroom for a lot of us in the mushroom space. They have been used by communities in West Africa and even in parts of North East India for many centuries as both an edible delight and also as folk medicine. These are not to be confused with the popular gourmet mushroom King Oyster (or Pleurotus eryngii) though they both share the characteristic feature of growing from the top surface of the substrate, rather than sideways like your conventional Oyster mushrooms do. Apart from this feature King Tuber and King Oyster mushrooms are two very different mushrooms. This mushroom derives its name from its unique ability to form truffle-like tubers underground, which are actually hard bundles of mycelium called sclerotia (which also act as food reserves for fungi). Apart from being a culinary delight, sclerotia contain a considerable amount of polysaccharides that are responsible for the multitude of medicinal properties that these mushrooms exhibit. If these facts haven’t blown your mind yet, let me tell you more!

 

Heat Tolerant Mushroom

King Tuber Oyster mushrooms are one among the most heat tolerant cultivable mushrooms out there, capable of withstanding temperatures up to 40 degrees Celsius! Now that’s one hot mushroom! With temperatures around the world increasing year after year, the effects of global warming cannot be dismissed. Keeping this in mind, these mushrooms have the potential to become a food source in places where other crops might find it hard to survive due to high temperatures.

These mushrooms have a lot of potential for commercial cultivation but a lot more research needs to be done to develop commercial strains which have superior yields before these mushrooms can become available to the masses. As of today, these mushrooms are a rare delicacy that is wild foraged from tropical forests in Africa, Asia, and Australasia.

We even have tribal communities in Tripura who use these wild-harvested mushrooms to cook up some amazing delicacies.

 

In the wild, King Tuber Oysters can be seen growing from rotting wood or from the soil. In the forests of Nigeria, these mushrooms can be seen growing around the African breadfruit tree. They are considered primary decomposers and can be cultivated on lignocellulosic waste similar to other gourmet and medicinal mushrooms. In their natural habitat, these mushrooms first form a thick bundle of mycelium underground, the sclerotia. Under favorable conditions, the fruiting bodies start to emerge from the sclerotia and appear above ground as beautiful light ochre mushrooms with funnel-shaped caps. These caps turn flat and proceed to turn wavy after the mushrooms reach full maturity. The sclerotia can be harvested and used to grow these mushrooms by simply planting it inside the soil, the same way you do with seeds!

Medicinal Uses 

Traditionally these mushrooms have been used in folk medicine in Ghana (4) for the treatment of a variety of health conditions including asthma, and high blood pressure and even to assist in weight gain for malnourished children. For all these purposes the mushroom/sclerotia is dried and powdered first and then used in soups or broths or even as an additive to flour used in cooking. One quick search for this mushroom on google will leave you with a multitude of research papers that show the therapeutic benefits of this special fungus.

King Tuber Oyster mushroom contain bioactive molecules which have been shown to help with-

• Lowering high blood pressure (2)
• Lowering high blood sugar (1)
• Lowering Cholesterol (1)
• Anti-Tumor/Cancer: (2,5)
• Anti-microbial properties (for Herpes Simplex) (2) (5)
• Anti-Obesity (2)
• Liver protection (2, 5)

Apart from medicinal benefits, King Tuber Oysters are also very high in potassium(3), anti-oxidants (2), and dietary fiber (equivalent to legumes or even seaweed)(5). It is an excellent prebiotic (2) and has also been explored as one of the few vegetarian sources of Glucosamine (5) which is used by patients suffering from various joint, bone, and inflammatory diseases such as arthritis.

Another really interesting property of this mushroom is its ability to break down polyethylene, which is a commonly used type of plastic! Don’t believe me, see the result of the study for yourself! As can be seen below, Pleurotus Tuber regium was able to decrease the weight of the polyethylene strips by a good 13.25% which is almost 50% higher than that compared to Pleurotus pulmonarius.

So we have with us a rare mushroom that grows in tropical jungles, is capable of withstanding scorching high temperatures, has the potential to treat a multitude of health conditions, and can eat plastic to top it off it also happens to be super tasty!

The obvious question most of you have right now would be where can I find these mushrooms? Or how do I cultivate these mushrooms at home? Don’t worry, we got you covered! Over the last year, our R&D team at Nuvedo HQ has been busy tinkering and finding a way to bring this powerhouse of a mushroom to your hands. After a lot of trial and error, we were successfully able to come up with the cultivation technology for this amazing mushroom which is now available for you to experience at home in the form of an easy-to-use grow kit!

You can grab one here: https://nuvedo.com/product/king-tuber-oyster-mushroom-growing-kit/

One last point that I want to touch upon before concluding is the culinary nature of King Tuber Oyster mushroom. Due to its high fiber content, the texture of the caps can be quite chewy compared to other mushrooms. Also, the stipe of the mushroom is hard and difficult to chew but brings in a unique texture, unlike any other variety. Throwing the stem away is not advised because there are a lot of polysaccharides and bioactive molecules present in it. You can chop them up or put them in the blender and make a paste out of it, which can later be added to soups or brother. The blended mushroom can be used in a similar fashion as a grated coconut!

Before you start exploring this blog, I am assuming that all of you are familiar with the basics of what mushrooms are and how they are cultivated. If you’re new to this, please check out these blogs to know more about mushroom cultivation and the jargon used:

• Lifecycle of a Mushroom
• Glossary | All things Fungi
• 3 tips for the Best Oyster Mushroom Growing Experience

Mushroom cultivation by its very nature is sustainable and has a positive impact on the environment. Just by cultivating mushrooms, we upcycle a lot of agricultural waste which would have otherwise been disposed of or burnt. Mushrooms also use only a fraction of water used by commercially cultivated crops.

What makes things better? The substrate leftover after growing mushrooms is a really good compost starter!
If you want to explore the positive environmental impact of mushroom cultivation then this blog is for you: Why India Needs More Mushroom Farmers: Environment

But all things said, the resting guilt most mushroom cultivators face, is the usage of single-use plastics. In this blog, we are going to look deeply at the use of plastics in the mushroom industry, viable alternatives, and advancements in the field of biopolymers that may be possible solutions.

To begin with – let’s get down to basics.

Why does one need to use plastics in mushroom cultivation in the first place?

Here are the 5 main uses for plastics in the mushroom cultivation process:

1. Prevent evaporation: Most of the commonly cultivated mushrooms are composed of ~80% water. This water is absorbed by the mushroom from the sterilized or pasteurized substrate which is hydrated to hold 55 – 70% water, as a percentage of the total weight of the substrate. The plastic acts as a physical barrier to ensure that the water present in the substrate doesn’t get lost due to evaporation.
2. Protect against contamination: Plastic is inorganic. It lacks the nutrition and hence doesn’t support the growth of any unwanted microorganisms that can negatively affect the growth of mycelium.
3. Keep pests out: Plastic acts as a physical barrier to stop pests from entering and eating up or contaminating your substrate.
4. Protect against physical damage: The plastic cover ensures that the loose substrate stays intact while it is being moved around and while the mycelium slowly spreads through the substrate making it one single mass.
5. Provide suitable microclimate: Certain mushrooms like Antler Reishi (shown in the picture below), King Oyster & Enoki need a specific microclimate (high in C02 for example) to grow a certain way, the plastic helps in maintaining certain cultivation parameters.

 

 

Are there ways to grow mushrooms without using plastic?

Now that we’ve established the uses of plastic, another important question we need to address before we proceed is, are there any established methods of cultivating mushrooms without the use of plastics? Yes! there are plastic-free ways to grow mushrooms, the most common methods being log/totem cultivation and bed cultivation. In log/totem cultivation, logs of suitable trees and the right dimensions are inoculated with mycelium. The mycelium then feeds on the log and colonizes it, giving out mushrooms when the weather is right. Many mushrooms such as Shiitake, Oyster, and Reishi can be grown outdoors using logs.

 

 

The major disadvantage with this method is that the cultivator has to wait a year or two before his first harvest and also the fact that harvests will be seasonal, making it hard to cultivate mushrooms throughout the year.

Finding the right wood, at the right time of the year and in the required dimension can be hard. Another challenge with logs is that they are heavy, moving and handling them can be very energy-intensive. Apart from logs, outdoor beds are also a great way to grow mushrooms without the use of plastics. In this method, the cultivator makes an outdoor bed using a pasteurized substrate that has been layered with spawn. One major drawback of this method is that the number of species that can be cultivated is very low, for example, Paddy Straw, Wine Caps, and certain species of oyster mushrooms.

Growing in beds can also lead to increased chances of contamination and attacks by pests. Both methods described above are still used in different parts of the world today and can be viable for the cultivator depending on his location and requirements but for a cultivator looking for consistent yields and year-round production, bag cultivation remains the most practical option.

Let’s dig a little deeper and understand what are the different types of plastic available in the market and which ones are suitable for growing mushrooms.

Based on what they are made of and how they degrade, plastics can be put in one of 4 categories:

• Bio-based and Biodegradable: These plastics are derived from natural sources and are biodegradable. Eg: PLA, PHA, PBS, Starch blends, etc
• Non-bio-based and Biodegradable: These plastics are derived from artificial sources and are biodegradable. Eg: PBAR, PCL, etc.
• Biobased and non-biodegradable: These plastics are derived from natural sources but are not biodegradable. Eg: Bio-based PET, PE, PTT, etc
• Non-bio-based and non-biodegradable: These plastics are derived from artificial sources and are not biodegradable. Nearly all conventional plastics fall into this category.

 

This categorization makes it very clear that just because something is made from a natural source doesn’t mean that it is automatically biodegradable and on the other hand just because something is made artificially it doesn’t necessarily have to be biodegradable. Another common mistake is thinking that compostable and biodegradable imply that if you simply throw away that piece of plastic, it will degrade by itself. This is incorrect, the words “compostable” and “biodegradable” imply that it can be composted or degraded under industrial conditions for composting or bio-degradation!

This brings us to our next question, what is the most suitable type of plastic available for mushroom cultivation and why?

Most cultivators prefer using Polypropylene number 5 or PP5 for short. PP5 is commonly used as packaging for foodstuffs and as containers for food as it is a very safe and stable polymer that doesn’t degrade easily. It is the safety coupled with the fact that it can withstand temperatures of 121 degrees and 15 PSI pressure with ease, (this temperature and pressure are encountered in an autoclave which is typically used to sterilize substrate bags) that makes polypropylene bags for mushroom cultivation most popular. Unlike PP5, biodegradable polymers such as PLA, PHA, etc. cannot withstand such high temperatures or pressure and have poor barrier properties. This makes these biodegradable mushroom bags unsuitable for mushroom cultivation commercially.

 

This being said, if you’re pasteurizing your substrate and can find a way for the substrate to retain moisture, you can try using biodegradable polymers available in the market for mushroom cultivation. Post use, they need to be sent to the right facility to be composted. There are scientific papers out there that have examined the ability of certain plastic eating mushrooms (some oyster mushroom species for example) to degrade bioplastics such as PLA. Keeping this in mind, please be sure that you are using a type of bioplastic which the mushroom mycelium cannot degrade! To know more about this please read this article.

A few materials that we often get asked about for mushroom cultivation are jute bags and clay/ceramic pots. Both of these aren’t viable alternatives to plastic. Jute bags are porous, so evaporation will not be controlled and since it is made from plant matter they can get contaminated very easily. As for earthen pots, they are full of micropores which can act as a surface to attract contaminants, and similar to jute, the porous nature of the material will result in a loss of moisture from the substrate. Apart from this, clay/ceramic pots are fragile and heavy, handling them without damage can become an issue if they are used at a commercial level.

Though plastics are bad for the environment, there is no denying that it is a really good material that has a lot of uses and is economically viable for mushroom cultivation. One viable alternative that a mushroom cultivator can try is growing mushrooms in containers. These reusable PP5 containers have a long life and can be used over multiple cycles without any need to be replaced. Mind you, this alternative may not be suitable for all varieties of mushrooms and there will be an additional step of cleaning and maintaining these buckets, which will need additional resources. For example: Growing King Oyster mushrooms in buckets will not work the same way as other oyster mushrooms because King Oysters grow vertically from the top as compared to other oyster mushrooms which grow from the sides. For that matter growing Shiitake mushrooms in buckets is not a good idea either since the blocks need to be removed from the buckets to initiate fruiting.

 

 

 

 

 

 

 

 

 

Here are some representations of the same: Enoki mushrooms and King Oyster mushrooms being cultivated in reusable mushroom growing containers:

While writing this I am hopeful about the future and I feel that it is just a matter of time before performance-based bio-materials become widely available and cost-effective enough to replace plastics altogether in the mushroom cultivation process. Mushroom cultivators around the world are rooting for the day when biodegradable mushroom bags for cultivation will become widely available. We might even have completely sustainable and biodegradable mushroom plastic or mycelium based plastic alternatives which can be used for mushroom cultivation in the future. Till then, let’s all be on the hunt for more sustainable materials and practices, and let’s all do our bit in growing the mycelium network!

The ethical and environmental devastation wreaked by animal agriculture or livestock farming is finally getting mainstream attention in the West, and now in India. The impact of industrial animal agriculture is enormous. It emits 14.5% – 16.5% of the world’s greenhouse gas emissions, occupies over 30% of the Earth’s land, and has destroyed over 70% of the Amazon rainforest. It commodifies the lives of over 80 billion land animals in a year — raising and slaughtering them in intensive, cruel, and filthy conditions. It has been the breeding ground for avian and swine flu epidemics. Experts cite that the next pandemic could well originate from a factory farm. Can we afford this destruction just to satiate our demand for meat?

Today, there are several humane and sustainable alternatives to animal-based meat. They use a fraction of the environmental resources required by conventional meat, do not lead to fatal contagious diseases, and spare billions of animals unnecessary suffering. Plant-based alternatives to meat, dairy, and eggs have proved that they are no longer just a passing health or sustainability fad — they are no longer consumed only by vegans or health enthusiasts.

Veganuary was started as a challenge by a UK nonprofit group in January 2014 to help people transition to a plant-based diet for ethical, environmental, and health reasons. Veganuary India offers support in this transition too.

The future is plant-based and these alternatives are here to stay! Gone are the days when meat alternatives consisted merely of soya nuggets or chaap, or tofu analogues. Meat alternatives today mean business — they are tasty, affordable, and accessible, and will only get better with increase in demand.

The humble mushroom is playing a crucial role in mitigating the disastrous impacts of animal agriculture, and in forging a new food system that is more equitable for people and animals. It is rich in potassium (which reduces sodium levels and blood pressure), contains B vitamins which can boost energy, has anti-inflammatory effects on our immune systems, and is loaded with antioxidants and polyphenols that protect against aging.

Mushrooms have enormous potential as raw materials for plant-based meats, leading to an explosion of mushroom-based meats in the West. According to Forbes, investors are favouring fungi-based meats as they have fewer ingredients, require significantly less processing, and have a better nutritional profile than the current popular plant-based meat ingredients such as soy or pea. According to The Good Food Institute, an impressive USD 1.5 billion was invested in plant-based meats in 2020, of which USD 435 million was in fermentation technologies, which are used by several mushroom startups. Meati is one such example. It is grown indoors, without environmental pollutants or antibiotics and is ‘brewed’ using water, sugar, and nutrients from the mushrooms’ mycelium structures. Meati uses this technology to create its steak, chicken, and jerky. Ecovative is another company that created its AirMycelium™ platform which systematically grows mycelium fibres in specific patterns using a range of biological processes to create mushroom-based meat and leather at scale. It then created MyForest Foods to launch its first product MyBacon, using its gourmet mycelium.

Mushrooms are an ideal raw material for meat alternatives — they are grown extensively across India, are not resource-intensive, are nutritionally dense, and provide a texture and umami flavour that are convincingly meat-like. For a population that is growing increasingly conscious of their health and the environment, it is only a matter of time (and increasing consumer demand) that such products are available and price-competitive in India!

Mushrooms are playing a vital role in reforming the traditional leather industry too. India’s dairy, beef, and leather industries are inextricably interlinked with each other. These industries have the same ‘raw material’, i.e. India’s cows and buffaloes. In addition to the obvious cruelty to ~ 500 million animals, the cattle industry’s greenhouse gas emissions are amongst the highest in the world, with India being among the world’s top beef and dairy exporters and consumers. The industry is infamous for emitting sludge and metals like chromium, lead, zinc, and manganese into nearby water bodies. It is also rife with social ills. According to the report ‘Do Leather Workers Matter?’, Dalits and Muslims, who comprise a majority of tannery workers, must contend with abysmal wages and working conditions — leading to eye infections, skin diseases, lung ailments, and cancers. Such workers are not offered health insurance, and in case of accidents, are rarely provided compensation.

Mushrooms have superior characteristics that make them a natural choice for leather alternatives. The cruelty-free and biodegradable nature of mushrooms allay the ethical, environmental, and human rights concerns associated with traditional leather. Mushroom-based leathers today are touted to be at par with traditional leather in terms of performance.

One of the world’s leading mushroom leather companies is Bolt Threads, which produces Mylo, a mycelium-based material, currently ready for commercial production. “Growing a cow takes one to two years. It takes growing the feed before you grow the cow. And so you’ve got a huge impact embedded in that cow. Mylo takes less than two weeks to grow.”, says Jamie Bainbridge, Vice President of Product Development at Bolt, to Fast Company. Bolt will not release further information on CO2 savings and a life cycle analysis until production starts at scale. MycoWorks, another company, claims to innovate over existing mushroom-based leathers in its patented Fine Mycelium™ technology, which “engineers mycelium cells as they grow to create three dimensional structures that are densely entwined and inherently strong”. This creates the chromium-free final leather product Reishi, which compares with the durability, performance, and environmental impact of animal-based leathers. In March 2021, MycoWorks collaborated with luxury brand Hermes to launch the Victoria travel bag, bringing its biotechnological innovation and Hermes’ legendary craftsmanship together to design the future of fashion.

In addition to providing ethical and sustainable alternatives to the leather industry, mushrooms offer us an opportunity to consider our consumption practices overall. As Merlin Sheldrake, author of ‘Entangled Lives: How Fungi Make Our Worlds, Change Our Minds, and Shape Our Futures’ told The Guardian, “We have been trained as consumers to think in terms of a straight line whereby we buy something, use it and throw it away. This is about material innovation, but it’s also about the culture of making endless new things, and what we can learn from thinking in terms of nature and of cycles instead.”

In addition to the immense potential that mushrooms offer as alternative meats and materials, one of its most important benefits is the potential economic opportunities for traditionally marginalised communities in a developing country like India. India’s poultry industry comprises huge conglomerates that employ millions of farmers through contract-farming agreements. India’s version of The Transfarmation Project could provide such farmers with an opportunity to escape the debt associated with vertically-integrated chicken farming, and transition to the significantly more humane and sustainable mushroom-farming model. This in turn could supply mushrooms as raw material for India’s burgeoning plant-based meat industry.

Mushrooms are leading the way when it comes to sustainable food and materials globally, and I expect that it will do so soon in India too. In honour of Veganuary, I urge you to give mushrooms a try. Watch this space as Nuvedo brings mushrooms to the forefront of India’s sustainable food and materials systems!

 

 

 

 

 

 

Socio-economic-issues

Unemployment

India is said to have over 38 million unemployed people as per reports published in December 2020. Though this number is disputed, it is still alarmingly high and unemployment continues to be the largest social issue in India.

 

Source: https://www.businesstoday.in/latest/economy-politics/story/india-unemployment-rate-april-cmie-highest-257055-2020-05-01

Mushroom cultivation is an extremely labor-intensive process and this is precisely what makes it expensive to cultivate mushrooms in the USA and Europe. The whole process can only be automated partly and there are critical parts of the growing cycle that need human intervention. In India, we have a wealth of human resources, both skilled and unskilled, which can be utilized to fuel the growing international demand for cheap and high-quality mushrooms. With the right training and supportive ecosystem, India has the potential to turn into the mushroom cultivation hub of the world.

More people getting into controlled cultivation of fungi can create a multitude of jobs across the sector, which can address the issue of seasonal unemployment currently present in the agricultural sector. With more investment into the larger mushroom ecosystem, like developing value-added products or manufacturing spawn, more job opportunities will get created across the whole mushroom value chain.

Women empowerment

There is a need today more than ever to redefine the status of women in Indian society.  Economic empowerment through financial independence is one of the ways in which we can help women gain access to their rightful share of things in different spheres of life. We can do this by giving women access to resources that can help them generate a stable source of income. Without economic strength or reasonable income security women will always lack the freedom to make rational choices and to become socially responsible.

Some mushrooms such as oysters can be cultivated at home with low technical inputs and skills, due to new cultivation techniques like cold water pasteurization which removes the need for any equipment. Oyster mushrooms grow fast (one complete cycle in 45 days), are also quite hardy, and can tolerate changes in an external environment to a larger extent compared to other mushrooms. Therefore, the cultivation of oyster mushrooms at home is a relatively fast and easy way for women to earn a livelihood and supplement their income from other sources such as rearing cows or chicken farming which are the current favorites among women to make a living. As long as the production volumes are commercially viable and they have access to a steady market, small-scale cultivation of mushrooms is a viable option. A single household can make up to 1 ton of oyster mushrooms in a cycle with just 600 sq ft of dedicated growing area. The synergy of a group is much higher than an individual, so adopting a participatory approach like setting up self-help groups centered around mushroom cultivation can definitely help make women an equally important paradigm of the development process.

It is safe to say that mushrooms are in line with our current agro-economy and can play a vital role in serving many needs such as environmental, socio-economic and health which are key to a healthy, flourishing country. As Indians, we have to look deeper into the mycoverse, understand fungi and their benefits and adapt them suitably to our current climates and weather patterns. For consumers to help grow this ecosystem and support farmers, encouraging conversations surrounding mushrooms and adapting them in our diets is equally important if not, vital to the survival of mushroom cultivation industry. 

If you’d like to recap on the other two parts that addressed two key areas:

Part 1: Environmental – Read here: https://nuvedo.com/2021/12/20/why-india-needs-more-mushroom-farmers-part-1-3/

Part 2: Health & Wellbeing – Read here: https://nuvedo.com/2021/12/29/why-india-needs-more-mushroom-farmers-part-1-3-2/

Health & wellbeing

“Mushrooms are miniature pharmaceutical factories, and of the thousands of mushroom species in nature, our ancestors and modern scientists have identified several dozen that have a unique combination of talents that improve our health.”
– Paul Stamets

Protein deficiency in the Indian diet

The Indian Market Research Bureau’s 2017 report states that measured against the recommended daily intake of 60g, 80% of Indians are protein deficient. The usual sources of proteins in a regular Indian diet consist of one cup of lentils, 1 glass of milk, or 1 cup (200 g) of yogurt which contains 7-8 grams of protein. In order to meet the requirement of 60g protein per day, it would be necessary to eat eight bowls of lentils or drink 7-8 glasses of milk which you and I both know is practically impossible!

 

Mushrooms are considered the highest producer of protein per unit area and time. This basically means that for a given area and a fixed time frame, compared to all other sources, mushrooms have the highest protein content. So, just how much protein does a mushroom contain? 100 g of dried Lentinus Sajor Caju commonly known as the Indian oyster mushroom contains around 28 grams of protein, which is as much as you would find in 100 g of chicken breast! Apart from absolute protein content, the quality of protein from mushrooms is superior to almost all other vegetarian sources. This is because mushrooms contain all of the 9 essential amino acids whereas most plant-based protein sources lack one or more of these or may not have them in the required quantities.

 

If you’re an environmentally conscious fitness enthusiast, you might want to swap your chicken steak for some mushrooms next time because it takes roughly 156 times the amount of water to produce 1 kg of chicken as compared to the same quantity of fresh mushrooms. Yet one more way in which mushroom cultivation can help society is by giving us the protein we need on a daily basis while using only a fraction of water and resources compared to popular alternatives.

By adopting mushrooms as part of our daily diet, we can bring about the much-needed shift towards vegetarian and sustainable sources of protein at an individual level. This can further encourage more farmers to take up mushroom cultivation as a means to support themselves in a more sustainable manner.

 

Increase in lifestyle disorders as the leading cause of death in India

A recent article by the business world states that nearly 61% of people in India die due to lifestyle diseases. Lifestyle diseases are ones that are connected to how people live their lives. Irregular sleep patterns, unhealthy food habits, lack of regular physical activity, and stress are the leading causes of lifestyle disorders. Major lifestyle disorders most prevalent in India are obesity, hypertension, heart disease & stroke, type-2 diabetes, cancer, and Alzheimer’s.

With advances in technology and the ever-increasing pace of life, lifestyle disorders are here to stay. Which leads us to the question of how mushrooms could possibly play a role in this?

Well, it turns out that many mushrooms contain novel compounds which can help us treat or manage many of the disorders mentioned above. Let’s take a look at a few common examples of medicinal mushrooms and their benefits:

 

 

From the above list, it is quite obvious that adding a dose of some of these potent medicinal varieties of mushrooms to your daily diet can help prevent or treat many of the most prevalent lifestyle disorders of today. No wonder mushrooms such as Reishi, which is regarded as the mushroom of immortality in China, have been used in traditional Chinese medicine for the last 10,000 years! India, with its rich heritage of natural remedies, should seriously consider adding these powerful medicinal mushrooms to its already vast arsenal of herbs.

Promoting awareness about the healing potential of mushrooms and their uses can help increase access to alternative healing remedies, which can improve the quality of life of millions of Indians who seek relief from their health conditions on a day-to-day basis.

I’ll be taking into account the other issue in the next blog piece stay tuned folks for Part 3: Why India Needs More Mushroom Farmers.

Read Part 3 here: https://nuvedo.com/2022/01/05/why-india-needs-more-mushroom-farmers-part-3-3/

If you need to recap on Part 1 on some of the environmental issues Read Part 1 here: https://nuvedo.com/2021/12/20/why-india-needs-more-mushroom-farmers-part-1-3/

Our tagline at Nuvedo is “In Mushrooms We Trust”. At Nuvedo, we truly believe that fungi hold the answer to many of the most pressing issues that our country India is facing today on multiple fronts. Rather than looking at these as issues, we can approach them as opportunities for us to harness the true potential of fungi and progress towards a more sustainable future. So, let us take a look at some of these opportunities and see how mushrooms can help solve them.

The problems that we face today can be broadly be classified into 3 categories:

• Environmental
• Health and Wellbeing
• Socio-economic

Though all the problems listed below can be considered to have an environmental and economic benefit owing to the very nature of mushrooms, I have categorized them on the basis of the dominant theme. This blog has been split into 3 parts so as to get into the details of each of them. In part one, the environmental angle is what will be explored.

Environmental

The issue of crop burning

Every year, India generates about 700 million tons of agricultural waste out of which close to 16% is burnt, leading to an increase in airborne pollutants.

How bad is this problem?

It is estimated that the total national annual emission for CO2 from crop residue burning alone is more than 64 times the total annual CO2 pollution emission in Delhi. If this isn’t alarming enough, a study conducted by the Institute for Social and Economic Change, Bengaluru, estimates that people in rural Punjab spend INR 7.6 crore every year on treatment for ailments caused by crop residue burning. Even after three years of an intense awareness campaign in Punjab, along with INR 1,000 crore worth of subsidies on straw management machines, there hasn’t been any big shift away from stubble burning. As per the data from Nasa’s VIIRS 375m satellite, 86,606 fires were detected in Punjab and Haryana this year which marks a 7.3% increase over last year. Moreover, climate scientists have already linked fine particulate matter in the haze to the melting of Himalayan glaciers. So, it is quite clear that crop residue burning is not a problem to be taken lightly. To understand the underlying causes let’s try and understand why farmers resort to burning crop residue.

There are 3 main reasons

1. Most farmers use combine harvesting machines which cut the crop eight inches above the ground, leaving the stem stuck in the soil. Additional machinery is required to further remove the stubble, which have high operating costs and hence increases the financial burden on farmers. Burning is the cheapest and most convenient way to dispose of crop residue due to which farmers resort to it.
2. There is no other profitable source of income that the farmer can generate from agricultural waste, apart from selling it as feedstock for bio-CNG plants or as animal fodder, both of which don’t generate enough income to cover the labour involved.
3. The time window available to prepare the fields and sow winter wheat crops after paddy harvesting is really small, which forces the farmers to find a fast and easy way to remove crop residue. Overall, farmers find that harvesting the crop residue simply isn’t worth the effort that goes into it.

This is where mushrooms come in. Agricultural waste such as paddy and wheat straw makes a great substrate for cultivating a variety of mushrooms including different types of Oyster, Paddy straw, and Milky mushrooms. These mushrooms digest the lignin, cellulose and hemicellulose present in the straw and turn it into nutrients that help them grow. To see how profitable it can be let us compare the wholesale price (as of December 2021) of 1 Ton of wheat, Onion, and Oyster Mushrooms; 1 ton of wheat sells for  ₹ 21,000, Onion for  ₹ 62,000, and Oyster mushrooms for  ₹ 1,00,000! Farmers can use their crop residue to cultivate these lignin-loving mushrooms and generate a considerable chunk of revenue from what would otherwise have been burnt. What makes this even more economical is that the mushrooms substrate which has been utilized to grow mushrooms (also called spent mushroom substrate) can be used in compost preparation; which can be added back into the fields to improve the structure and soil fertility. Currently in India only 1.5% of the agricultural waste generated is being used as substrate to cultivate mushrooms.

The spent mushroom substrate has also found use in industries to generate bio-diesel or can even be used to feed livestock! As you can see, mushroom cultivation is something that should be promoted in areas with an abundance of agricultural waste as a way to reduce waste, stop pollution and generate additional income.

 

India’s water crisis

India is facing a major water crisis today, which can be attributed to the culmination of a variety of issues. The major cause of this crisis lies within the agricultural industry. Nearly 90% of the available groundwater is currently being used by the agricultural industry for irrigation. This issue is made worse by unscientific growth of water intensive crops like sugarcane and rice in areas with poor rainfall and insufficient ground water. The Nature study, published in the year 2009, clearly warned that, if counter measures are not taken soon, India would not only face severe shortage of drinking water, but the agricultural yields would also reduce, which could lead to extensive socio-economic issues. The evidence so far is in line with this prediction. For example, the agricultural yield growth of Punjab which was once the top performing state, has reduced considerably in recent years. This coupled with potable water shortage and water and air pollution has caused a variety of problems in the state.

Consider this- 1 kg of wheat needs 1350 liters of water, 1 kg of rice needs 3000 liters of water, 1 kg maize needs 900 liters of water. Compared to all the crops mentioned above, 1 kg of fresh oyster mushrooms requires just 25 liters of water! Need I say more? Mushroom cultivation could be a really good way to provide much needed nutrition to the average Indian while utilizing 90% less water compared to commercially cultivated crops.

 

I’ll be taking into account the other two issues in the next blog piece stay tuned folks for Part 2: Why India Needs More Mushroom Farmers.

Read Part 2 here: https://nuvedo.com/2021/12/29/why-india-needs-more-mushroom-farmers-part-1-3-2/